Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HU772_RS23310 Genome accession   NZ_CP077095
Coordinates   5247543..5248538 (-) Length   331 a.a.
NCBI ID   WP_186656667.1    Uniprot ID   A0A9E6PW34
Organism   Pseudomonas xantholysinigenes strain RW9S1A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5242543..5253538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU772_RS23290 (HU772_023290) argS 5243608..5245344 (+) 1737 WP_186656658.1 arginine--tRNA ligase -
  HU772_RS23295 (HU772_023295) - 5245346..5246047 (+) 702 WP_186656660.1 SPOR domain-containing protein -
  HU772_RS23300 (HU772_023300) - 5246134..5246559 (-) 426 WP_186656663.1 TM2 domain-containing protein -
  HU772_RS23305 (HU772_023305) - 5246717..5247367 (+) 651 WP_186656665.1 C40 family peptidase -
  HU772_RS23310 (HU772_023310) pilT 5247543..5248538 (-) 996 WP_186656667.1 type IV pilus twitching motility protein PilT Machinery gene
  HU772_RS23315 (HU772_023315) - 5248603..5249289 (+) 687 WP_186656669.1 YggS family pyridoxal phosphate-dependent enzyme -
  HU772_RS23320 (HU772_023320) proC 5249328..5250146 (+) 819 WP_186656671.1 pyrroline-5-carboxylate reductase -
  HU772_RS23325 (HU772_023325) - 5250157..5250747 (+) 591 WP_138220469.1 YggT family protein -
  HU772_RS23330 (HU772_023330) - 5250836..5251975 (+) 1140 WP_186656678.1 homoserine O-acetyltransferase -
  HU772_RS23335 (HU772_023335) metW 5251983..5252603 (+) 621 WP_134691337.1 methionine biosynthesis protein MetW -
  HU772_RS23340 (HU772_023340) - 5252629..5253063 (+) 435 WP_186656680.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35888.58 Da        Isoelectric Point: 6.8478

>NTDB_id=579199 HU772_RS23310 WP_186656667.1 5247543..5248538(-) (pilT) [Pseudomonas xantholysinigenes strain RW9S1A]
MDVTELLERALAAGASDLHLAVGERPLLRVDGVLQRLDFPVVTAVALERGMTALLNGGQRQQWLAADELDLALSLPDLGR
FRLNLFRQRAGLGASVRLIPAQVPNIEALDLVEVFEHIGRCRDGLVLVGGPTGSGKSSTLAALVDRLNRDYALHIVTLED
PIEVIHSGQRSLVNQREMGSHSRDFVQGLCSALRQDPDVIVVGELRDLESIRLALRAAETGHLVLATVHTRSALNSIDRL
VEVFAPEEKPLVRTMLAESLRLVVAQVLVQRRDGGRVAAREVLVATPAVRNLIREGRMTQIYSLMQTGAGVGMRTMEGAM
QALAARGLIVG

Nucleotide


Download         Length: 996 bp        

>NTDB_id=579199 HU772_RS23310 WP_186656667.1 5247543..5248538(-) (pilT) [Pseudomonas xantholysinigenes strain RW9S1A]
ATGGATGTGACTGAGCTGTTGGAGCGGGCATTGGCCGCAGGGGCCAGCGACCTGCATCTGGCCGTGGGCGAGCGGCCGCT
GCTGCGTGTCGATGGTGTGTTGCAGCGCCTGGACTTTCCGGTGGTGACGGCGGTGGCACTGGAGCGCGGGATGACTGCAT
TGCTCAATGGCGGGCAGCGTCAGCAGTGGCTGGCCGCGGATGAACTGGACCTGGCGTTGAGCCTGCCGGATTTGGGGCGT
TTTCGCTTGAACCTGTTCCGCCAGCGCGCTGGGCTGGGCGCCAGCGTGCGCTTGATCCCGGCGCAGGTGCCGAATATCGA
GGCGCTTGATTTGGTGGAGGTGTTTGAGCACATTGGCCGCTGCCGGGATGGCCTGGTGCTGGTGGGTGGCCCGACCGGCA
GCGGCAAGTCCAGCACCCTGGCCGCACTGGTCGATCGGCTGAACCGCGACTATGCGTTGCACATCGTCACCCTCGAAGAC
CCGATCGAAGTTATCCACAGCGGTCAACGCAGCCTGGTCAATCAACGTGAAATGGGCAGCCACAGCCGTGACTTTGTCCA
GGGCTTGTGCAGTGCCCTGCGTCAGGACCCGGATGTGATCGTGGTCGGTGAGCTACGTGACCTGGAGAGCATCCGCCTGG
CGCTGCGGGCGGCGGAAACCGGGCACCTGGTGCTGGCTACCGTGCATACGCGCTCGGCGCTGAACAGCATCGACCGGTTG
GTGGAGGTGTTCGCGCCGGAGGAAAAGCCGTTGGTGCGTACGATGCTTGCCGAGTCGCTGCGGCTGGTGGTCGCGCAGGT
GCTGGTCCAGCGCCGGGATGGCGGGCGTGTGGCGGCGCGTGAGGTGCTGGTGGCGACGCCTGCGGTGCGCAACTTGATCC
GCGAAGGACGGATGACGCAGATCTATTCGTTGATGCAGACCGGCGCGGGGGTGGGAATGCGCACGATGGAGGGGGCGATG
CAGGCGCTGGCGGCGCGGGGCTTGATAGTGGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.76

99.396

0.505
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.76

99.396

0.505
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.396

0.498
  pilT Pseudomonas aeruginosa PAK

49.848

99.396

0.495
  pilT Acinetobacter baylyi ADP1

49.544

99.396

0.492
  pilT Acinetobacter baumannii strain A118

48.936

99.396

0.486
  pilT Acinetobacter baumannii D1279779

48.936

99.396

0.486
  pilT Acinetobacter nosocomialis M2

48.632

99.396

0.483
  pilT Neisseria meningitidis 8013

47.879

99.698

0.477
  pilT Neisseria gonorrhoeae MS11

47.879

99.698

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.828

100

0.468
  pilT Legionella pneumophila strain Lp02

46.201

99.396

0.459
  pilT Legionella pneumophila strain ERS1305867

46.201

99.396

0.459
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.366