Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KSS95_RS04570 Genome accession   NZ_CP077073
Coordinates   990444..991433 (+) Length   329 a.a.
NCBI ID   WP_217852036.1    Uniprot ID   -
Organism   Pseudomonas muyukensis strain COW39     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 985444..996433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS95_RS04540 (KSS95_04540) - 985906..986340 (-) 435 WP_134691336.1 DUF4426 domain-containing protein -
  KSS95_RS04545 (KSS95_04545) metW 986366..986986 (-) 621 WP_011531735.1 methionine biosynthesis protein MetW -
  KSS95_RS04550 (KSS95_04550) - 986994..988133 (-) 1140 WP_217852031.1 homoserine O-acetyltransferase -
  KSS95_RS04555 (KSS95_04555) - 988234..988824 (-) 591 WP_134691339.1 YggT family protein -
  KSS95_RS04560 (KSS95_04560) proC 988835..989653 (-) 819 WP_217852032.1 pyrroline-5-carboxylate reductase -
  KSS95_RS04565 (KSS95_04565) - 989692..990378 (-) 687 WP_217852034.1 YggS family pyridoxal phosphate-dependent enzyme -
  KSS95_RS04570 (KSS95_04570) pilT 990444..991433 (+) 990 WP_217852036.1 type IV pilus twitching motility protein PilT Machinery gene
  KSS95_RS04575 (KSS95_04575) - 991483..992127 (-) 645 WP_217852037.1 C40 family peptidase -
  KSS95_RS04580 (KSS95_04580) - 992285..992710 (+) 426 WP_217852039.1 TM2 domain-containing protein -
  KSS95_RS04585 (KSS95_04585) - 992750..993451 (-) 702 WP_217852041.1 SPOR domain-containing protein -
  KSS95_RS04590 (KSS95_04590) argS 993453..995189 (-) 1737 WP_217852043.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 35570.35 Da        Isoelectric Point: 9.3431

>NTDB_id=578636 KSS95_RS04570 WP_217852036.1 990444..991433(+) (pilT) [Pseudomonas muyukensis strain COW39]
MDVTELLGQARAAGASDLHLAVGERPLLRLEGVLQRLALAPLTALVLEQGMAALLDDDQRRQWLAADELDLAVSLPGLGR
FRLNLFRQCNGLGASVRLIPAQVPNLEALELVDVFAAISRCRDGLVLVSGPTGSGKSSTLAALVDRLNRDHALHIVTLED
PIEVIHSGQRSLVNQREIGRHSRDFAQGLRSALRQDPDVIMVGELRDLESIRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAPQEKPLVRAMLAESLRLVVAQVLVRRRGGGRVAAREVLVATPAVRNLIREGRMAQLYSLMQAGGAVGMRTMEAAM
QALKAQGVL

Nucleotide


Download         Length: 990 bp        

>NTDB_id=578636 KSS95_RS04570 WP_217852036.1 990444..991433(+) (pilT) [Pseudomonas muyukensis strain COW39]
ATGGATGTGACCGAGCTGCTGGGCCAGGCCCGGGCGGCGGGTGCCAGCGACCTGCACCTGGCCGTGGGGGAGCGGCCGTT
GCTGCGCCTGGAGGGTGTGTTGCAGCGGCTGGCGCTTGCGCCGTTGACCGCGCTGGTGCTGGAGCAAGGCATGGCCGCGT
TGCTCGACGACGACCAGCGCCGGCAATGGCTGGCGGCAGACGAACTGGACCTGGCGGTGAGCCTGCCTGGGCTGGGGCGC
TTTCGGCTGAACCTGTTTCGTCAGTGCAATGGGCTGGGCGCCAGCGTGCGCTTGATCCCGGCGCAGGTGCCGAATCTCGA
GGCGCTGGAGCTGGTGGATGTTTTTGCAGCTATCAGCCGCTGTCGTGATGGCCTGGTGCTGGTGAGCGGGCCGACCGGCA
GCGGCAAGTCCAGCACATTGGCGGCGCTGGTCGACCGCCTCAACCGCGACCATGCGCTGCACATCGTCACCCTCGAAGAC
CCGATCGAAGTGATCCACAGCGGCCAACGCAGCCTGGTCAATCAGCGCGAGATCGGGCGTCACAGCCGCGACTTCGCCCA
GGGCCTGCGCAGTGCCCTGCGCCAGGATCCGGATGTGATCATGGTTGGTGAACTGCGTGACCTGGAGAGCATCCGCCTGG
CGCTGCGGGCAGCGGAAACCGGGCATCTGGTGCTGGCCACCGTGCATACACGCTCGGCGGTGAACAGCATCGACCGGCTG
GTGGAGGTGTTCGCGCCGCAAGAGAAACCATTGGTGCGCGCGATGCTCGCCGAATCGCTACGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGTCGCGGTGGCGGGCGTGTGGCGGCGCGTGAGGTGCTGGTGGCGACGCCTGCGGTGCGCAATTTGATCC
GCGAGGGACGGATGGCGCAGCTCTACTCGTTGATGCAGGCGGGCGGGGCGGTCGGGATGCGGACGATGGAGGCGGCGATG
CAGGCGCTGAAGGCCCAAGGTGTGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.456

100

0.505
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

100

0.505
  pilT Vibrio cholerae strain A1552

50.456

100

0.505
  pilT Pseudomonas stutzeri DSM 10701

49.848

100

0.498
  pilT Acinetobacter baylyi ADP1

49.24

100

0.492
  pilT Acinetobacter baumannii D1279779

48.936

100

0.489
  pilT Acinetobacter baumannii strain A118

48.936

100

0.489
  pilT Acinetobacter nosocomialis M2

48.632

100

0.486
  pilT Neisseria meningitidis 8013

46.97

100

0.471
  pilT Neisseria gonorrhoeae MS11

46.667

100

0.468
  pilT Legionella pneumophila strain ERS1305867

46.201

100

0.462
  pilT Legionella pneumophila strain Lp02

46.201

100

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.37

98.48

0.447
  pilU Pseudomonas stutzeri DSM 10701

37.195

99.696

0.371