Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PCNPT3_RS11555 Genome accession   NC_020802
Coordinates   2621466..2622602 (-) Length   378 a.a.
NCBI ID   WP_015466041.1    Uniprot ID   M4UAF3
Organism   Psychromonas sp. CNPT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2616466..2627602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCNPT3_RS11510 (PCNPT3_11110) - 2616901..2617641 (+) 741 WP_015466037.1 methyltransferase domain-containing protein -
  PCNPT3_RS11515 (PCNPT3_11115) rnhA 2617652..2618113 (-) 462 WP_015466038.1 ribonuclease HI -
  PCNPT3_RS11520 (PCNPT3_11120) dnaQ 2618170..2618880 (+) 711 WP_015466039.1 DNA polymerase III subunit epsilon -
  PCNPT3_RS11525 (PCNPT3_11125) - 2618868..2620109 (+) 1242 WP_015466040.1 hypothetical protein -
  PCNPT3_RS11555 (PCNPT3_11130) pilU 2621466..2622602 (-) 1137 WP_015466041.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCNPT3_RS11560 (PCNPT3_11135) glnS 2622875..2624530 (-) 1656 WP_015466042.1 glutamine--tRNA ligase -
  PCNPT3_RS14200 - 2624696..2624854 (-) 159 WP_156801535.1 hypothetical protein -
  PCNPT3_RS14350 (PCNPT3_11140) - 2624937..2625116 (-) 180 WP_015466043.1 PTS transporter subunit EIIB -
  PCNPT3_RS11575 (PCNPT3_11145) nagE 2625137..2626393 (-) 1257 WP_015466044.1 N-acetylglucosamine-specific PTS transporter subunit IIBC -
  PCNPT3_RS11580 (PCNPT3_11150) nagB 2626682..2627482 (+) 801 WP_015466045.1 glucosamine-6-phosphate deaminase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41834.23 Da        Isoelectric Point: 6.8512

>NTDB_id=57372 PCNPT3_RS11555 WP_015466041.1 2621466..2622602(-) (pilU) [Psychromonas sp. CNPT3]
MEFEEMLKILTNKDGSDLYLATGAPPCAKFQGNLKPLNAITLKPGEVAKIAEKLMNADQNITFEKDLEMNLAFSLSGIGR
FRINIFQQRNEVSIVVRNIKLDIPKFSDLGLPEVLKDVIMQKRGLVLFVGGTGSGKSTSLAALIDHRNSNASGHIITIED
PIEFVHRHKKSIVNQREVGIDTRSFKSALKNTLRQAPDVILIGEIRDRETMEHALTFSETGHLVLSTLHANNANQALDRI
INFFPEERRVQLLNDLGNNLQAFISQRLIKTKEGGRCAAIEVMIGTLTIRDMIKKGAFSDLKAMMEKSESAGMKTFDGAL
FDLVQQGKISEENALQNADSTTNLALKFKFAKGEILEPNGEGLTLKPNATSMDELEII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=57372 PCNPT3_RS11555 WP_015466041.1 2621466..2622602(-) (pilU) [Psychromonas sp. CNPT3]
ATGGAATTTGAAGAGATGCTTAAAATTTTGACGAATAAAGATGGATCGGATCTTTATTTGGCAACGGGAGCGCCACCTTG
TGCAAAGTTTCAAGGCAATTTAAAGCCTTTAAACGCAATTACTCTTAAACCTGGAGAAGTCGCGAAAATAGCTGAAAAGT
TGATGAATGCAGATCAAAACATCACTTTTGAAAAAGACTTAGAAATGAATTTGGCATTTTCACTTTCGGGCATTGGACGT
TTTCGTATTAATATTTTTCAACAACGTAACGAAGTGTCCATTGTTGTTCGTAATATAAAATTAGATATTCCTAAATTTTC
AGATTTAGGATTACCCGAAGTCCTTAAAGATGTGATCATGCAAAAACGCGGTTTAGTGTTATTTGTGGGCGGTACTGGAT
CAGGTAAATCGACATCATTAGCAGCGTTAATTGATCATCGTAATAGTAATGCAAGTGGGCATATTATTACCATTGAAGAT
CCCATTGAATTTGTGCACAGACATAAAAAAAGTATTGTTAACCAAAGAGAAGTGGGTATTGATACGCGCTCTTTTAAAAG
TGCTTTAAAAAACACGTTAAGACAAGCTCCCGATGTTATTTTAATTGGTGAAATTCGTGATCGTGAGACCATGGAGCACG
CTTTAACCTTTTCTGAAACGGGGCATTTGGTACTTTCAACATTACATGCTAATAATGCAAACCAAGCATTAGATCGGATC
ATTAACTTTTTCCCCGAAGAGCGCAGAGTGCAATTACTAAATGATTTAGGTAATAACTTACAGGCTTTCATATCACAACG
ACTCATCAAAACCAAAGAGGGTGGACGTTGCGCCGCCATTGAAGTGATGATTGGGACGCTGACCATTCGAGATATGATTA
AAAAAGGGGCCTTTAGTGATCTTAAAGCGATGATGGAGAAATCAGAATCTGCGGGTATGAAAACGTTTGATGGGGCGCTT
TTTGATTTAGTGCAACAAGGGAAAATTAGCGAAGAAAATGCACTTCAAAATGCAGACTCCACCACTAACCTTGCATTAAA
ATTTAAGTTTGCAAAGGGTGAAATATTAGAGCCTAATGGAGAGGGACTAACCTTAAAACCAAACGCAACGTCTATGGATG
AGCTTGAAATCATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4UAF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.324

97.884

0.532
  pilU Acinetobacter baylyi ADP1

49.153

93.651

0.46
  pilU Vibrio cholerae strain A1552

48.732

93.915

0.458
  pilT Pseudomonas aeruginosa PAK

43.314

91.005

0.394
  pilT Pseudomonas stutzeri DSM 10701

41.86

91.005

0.381
  pilT Legionella pneumophila strain Lp02

42.308

89.418

0.378
  pilT Legionella pneumophila strain ERS1305867

42.308

89.418

0.378
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.683

0.376
  pilT Acinetobacter nosocomialis M2

41.916

88.36

0.37
  pilT Acinetobacter baumannii D1279779

41.916

88.36

0.37
  pilT Acinetobacter baumannii strain A118

41.916

88.36

0.37
  pilT Acinetobacter baylyi ADP1

41.176

89.947

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.481

91.799

0.362
  pilT Vibrio cholerae strain A1552

39.481

91.799

0.362

Multiple sequence alignment