Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KME82_RS20725 Genome accession   NZ_CP076103
Coordinates   4976189..4977304 (+) Length   371 a.a.
NCBI ID   WP_036104874.1    Uniprot ID   A0A0Q8JJ88
Organism   Lysobacter capsici strain NF87_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4971189..4982304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KME82_RS20705 (KME82_20705) - 4972061..4973431 (-) 1371 WP_215495681.1 pyridoxal-phosphate dependent enzyme -
  KME82_RS20710 (KME82_20710) - 4974035..4974250 (-) 216 WP_031371506.1 YdcH family protein -
  KME82_RS20715 (KME82_20715) - 4975066..4975713 (-) 648 WP_215495682.1 hypothetical protein -
  KME82_RS20720 (KME82_20720) - 4975710..4976090 (-) 381 WP_215495683.1 DUF4398 domain-containing protein -
  KME82_RS20725 (KME82_20725) pilU 4976189..4977304 (+) 1116 WP_036104874.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KME82_RS20730 (KME82_20730) maiA 4977545..4978207 (-) 663 WP_215495684.1 maleylacetoacetate isomerase -
  KME82_RS20735 (KME82_20735) - 4978302..4979288 (-) 987 WP_215495685.1 fumarylacetoacetate hydrolase family protein -
  KME82_RS20740 (KME82_20740) - 4979769..4981736 (+) 1968 WP_215495686.1 peptidoglycan DD-metalloendopeptidase family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41139.45 Da        Isoelectric Point: 6.0537

>NTDB_id=571847 KME82_RS20725 WP_036104874.1 4976189..4977304(+) (pilU) [Lysobacter capsici strain NF87_2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSIIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNTNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFNESAHKNVLMNLALNLKAVVSQRLVVGVDGRRLPAAEILINTPHVRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDATAAH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=571847 KME82_RS20725 WP_036104874.1 4976189..4977304(+) (pilU) [Lysobacter capsici strain NF87_2]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACGACCGGCGCGCCTGTGTA
CATCAAGGTCGAGGGGCGTCTTTACCCGCTCGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCCTATTCCT
TGATGGACGAAGGCCAGGTGCCGCTGTTCGAGCGTGATCTCGAACTGAACATGGCCCTCGCCCTGCCCGACGCCGGGCGT
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCCATCCGCAGCATCATCCCGAGCATCGA
GGAGTTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCGCCGCGCGGGCTGGTCCTGATCGTCGGTTCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACCACCGCAACACCAACACCGCCGGCCATATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAAGCGCCCGACGTGATCCTGATCGGCGAAATCCTCGATGCGACGACCATGGAAGCGG
CGATCGCGTTCGCCGAAACCGGTCACCTGTGCCTGGCGACTCTGCACTCCAACAACGCCGACCAGACCCTGGAACGCATC
CTCAACTTCTTCAACGAGTCGGCGCACAAGAACGTGCTGATGAACCTGGCCTTGAACCTCAAGGCGGTGGTGTCGCAGCG
CCTGGTGGTCGGTGTCGACGGGCGGCGCCTGCCGGCGGCCGAAATCCTCATCAACACCCCGCACGTGCGCGACCTCATGC
GCCGCGGGCAGGTGCACGAGATCAAGCAGGCGATGGAAGAATCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGTCTGTACAAGGAAGGCCGGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCACCGGCGAGCACGATCCGTATGCGGATATGTATGACGCAACCGCGGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q8JJ88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

96.765

0.493
  pilU Acinetobacter baylyi ADP1

48.87

95.418

0.466
  pilU Vibrio cholerae strain A1552

47.458

95.418

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.608

91.375

0.426
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Pseudomonas aeruginosa PAK

41.124

91.105

0.375
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Neisseria gonorrhoeae MS11

38.905

93.531

0.364
  pilT Neisseria meningitidis 8013

38.905

93.531

0.364
  pilT Legionella pneumophila strain Lp02

39.645

91.105

0.361
  pilT Legionella pneumophila strain ERS1305867

39.645

91.105

0.361