Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KB891_RS13620 Genome accession   NZ_CP076049
Coordinates   2957439..2958578 (-) Length   379 a.a.
NCBI ID   WP_024569082.1    Uniprot ID   A0A2L0XCU8
Organism   Cupriavidus metallidurans strain YL2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2952439..2963578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KB891_RS13590 (KB891_13590) glcF 2953166..2954437 (+) 1272 WP_011517474.1 glycolate oxidase subunit GlcF -
  KB891_RS13595 (KB891_13595) - 2954576..2954962 (+) 387 WP_008641946.1 CidA/LrgA family protein -
  KB891_RS13600 (KB891_13600) - 2954959..2955684 (+) 726 WP_008641945.1 LrgB family protein -
  KB891_RS13605 (KB891_13605) - 2955770..2956150 (+) 381 WP_017514351.1 VOC family protein -
  KB891_RS13610 (KB891_13610) - 2956170..2956862 (+) 693 WP_008641943.1 helix-turn-helix transcriptional regulator -
  KB891_RS13615 (KB891_13615) - 2956889..2957383 (-) 495 WP_008641942.1 glutathione peroxidase -
  KB891_RS13620 (KB891_13620) pilU 2957439..2958578 (-) 1140 WP_024569082.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KB891_RS13625 (KB891_13625) pilT 2958596..2959639 (-) 1044 WP_008641940.1 type IV pilus twitching motility protein PilT Machinery gene
  KB891_RS13630 (KB891_13630) - 2959703..2960392 (+) 690 WP_017514353.1 YggS family pyridoxal phosphate-dependent enzyme -
  KB891_RS13635 (KB891_13635) proC 2960478..2961299 (+) 822 WP_024569083.1 pyrroline-5-carboxylate reductase -
  KB891_RS13640 (KB891_13640) ubiA 2961350..2962234 (-) 885 WP_017515416.1 4-hydroxybenzoate octaprenyltransferase -
  KB891_RS13645 (KB891_13645) - 2962349..2962834 (-) 486 WP_008641932.1 Dps family protein -
  KB891_RS13650 (KB891_13650) - 2962920..2963111 (-) 192 WP_008641929.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42559.97 Da        Isoelectric Point: 6.6193

>NTDB_id=571673 KB891_RS13620 WP_024569082.1 2957439..2958578(-) (pilU) [Cupriavidus metallidurans strain YL2]
MLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECNFA
IAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHIALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMVSFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=571673 KB891_RS13620 WP_024569082.1 2957439..2958578(-) (pilU) [Cupriavidus metallidurans strain YL2]
ATGCTCGACCGCGAATCCGCTGCAAAGTACATCAACGAAATGCTGGGGCTGATGGTCAAGAACCGTGGCTCCGATCTGTT
CATCACCGCGGAATTTCCTCCGGCCGTCAAGGTCGACGGGAAGATCACGCCGGTTTCCCAGCAGCCGTTGAATCCGACTC
ACGCGCTGACCCTGGTGTGTTCGATCATGAACGAGCGCCAGACCAAGGAGTTCGAGGACACCCGCGAATGCAATTTCGCG
ATCGCCGTGCCGGACGTCGGACGTTTCCGTGTCTCGGCCTTCATCCAGCAAGGCCGCGCGGGCATGGTGCTGCGGACCAT
CAACACCCGCATTCCATCGGTCGAAGACCTGGACCTGCCGCCGACGCTGCATGAGATCGTGATGTCCAAGCGCGGTCTTG
TGATCGTCACCGGCGCCACTGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGCTCGACCACCGCAACGCGCATTCGTAC
GGCCACATCATCACGATCGAGGACCCGATCGAATACGTCCACGCGCATCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
GATCGACACCGAGTCGTGGCACATCGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCGATGCAGTACGCGGAAACGGGCCACCTTTGCCTGGCCACGCTGCACGCGAACAAC
GCCAACCAGGCGATCGACCGTATCGTCAACTTCTTCCCAGAGGAAAAGCGCCAGCAGCTTCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCACGTGCCGGCCGCAAGGGCCGCGTGCCGGCAGTGGAGATCATGATCG
GCACGCCGCTAGTGGCTGACCTGATCTTCAAGGGCGAGATTCACGAGCTCAAGGAGGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTCTCGTTCGACCAGGCGCTGTTCGAACTCTACGAGACGGAGAAGATCACGTACGAAGATGCGCTCAAGAACGC
TGACTCGCTCAACGACCTGCGCCTGATGATCAAACTCCACAGCAAGCGCCATCGCGACAACGATCTCGGCGCCGGCACCG
AACACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0XCU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.348

0.578
  pilU Acinetobacter baylyi ADP1

59.429

92.348

0.549
  pilU Vibrio cholerae strain A1552

54.27

95.778

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

88.918

0.404
  pilT Legionella pneumophila strain Lp02

44.444

87.863

0.39
  pilT Legionella pneumophila strain ERS1305867

44.444

87.863

0.39
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter nosocomialis M2

44.514

84.169

0.375
  pilT Acinetobacter baumannii D1279779

44.514

84.169

0.375
  pilT Acinetobacter baumannii strain A118

44.514

84.169

0.375
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.641

0.367
  pilT Vibrio cholerae strain A1552

43.849

83.641

0.367