Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   KHW14_RS22530 Genome accession   NZ_CP074412
Coordinates   5157591..5158019 (-) Length   142 a.a.
NCBI ID   WP_043304828.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain U625     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5152591..5163019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW14_RS22505 (KHW14_22505) - 5153656..5154654 (-) 999 WP_122407783.1 ATP-grasp domain-containing protein -
  KHW14_RS22510 (KHW14_22510) - 5154774..5155325 (+) 552 WP_029243841.1 DUF1523 family protein -
  KHW14_RS22515 (KHW14_22515) - 5155560..5155991 (+) 432 WP_004887456.1 hypothetical protein -
  KHW14_RS22520 (KHW14_22520) - 5156047..5157027 (+) 981 WP_213673917.1 YiiG family protein -
  KHW14_RS22530 (KHW14_22530) pilA2 5157591..5158019 (-) 429 WP_043304828.1 pilin Machinery gene
  KHW14_RS22535 (KHW14_22535) pilB 5158245..5159945 (+) 1701 WP_096767277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW14_RS22540 (KHW14_22540) pilC 5159948..5161165 (+) 1218 WP_043304826.1 type II secretion system F family protein Machinery gene
  KHW14_RS22545 (KHW14_22545) pilD 5161167..5162039 (+) 873 WP_096767275.1 A24 family peptidase Machinery gene
  KHW14_RS22550 (KHW14_22550) coaE 5162036..5162659 (+) 624 WP_025993968.1 dephospho-CoA kinase -
  KHW14_RS22555 (KHW14_22555) yacG 5162656..5162865 (+) 210 WP_004887444.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14573.68 Da        Isoelectric Point: 8.4715

>NTDB_id=564748 KHW14_RS22530 WP_043304828.1 5157591..5158019(-) (pilA2) [Pseudomonas viridiflava strain U625]
MKAQKGFTLIELMIVVAIVGILAAVALPAYQDYTIRAQVAELIGLTDGAKVAVTENYQNTGVLPGTNAIAGYATTSGRYT
ASVNIGANGVITSVADAVNSNAAIRGQTITLTPTVVAGGNNVLTWACGGTIDVRFRPSSCRN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=564748 KHW14_RS22530 WP_043304828.1 5157591..5158019(-) (pilA2) [Pseudomonas viridiflava strain U625]
ATGAAAGCACAAAAAGGTTTTACTCTGATTGAGTTGATGATCGTCGTTGCGATTGTCGGTATTTTGGCGGCGGTCGCATT
GCCCGCATATCAGGATTATACGATTCGCGCGCAGGTGGCTGAGCTTATTGGCCTGACTGATGGCGCGAAGGTTGCTGTGA
CAGAAAACTATCAAAACACTGGTGTTCTTCCTGGCACGAACGCAATTGCTGGTTATGCTACTACCTCAGGACGTTACACA
GCATCCGTAAACATCGGTGCAAATGGCGTAATTACCTCTGTCGCTGACGCCGTAAACTCTAACGCCGCGATACGCGGACA
GACAATTACATTGACTCCAACTGTCGTTGCTGGTGGCAACAACGTTCTCACTTGGGCGTGCGGTGGCACCATTGACGTTC
GTTTCCGCCCAAGCAGCTGCCGAAATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55

98.592

0.542
  pilA2 Legionella pneumophila strain ERS1305867

54.286

98.592

0.535
  pilE Neisseria gonorrhoeae MS11

45.86

100

0.507
  pilE Neisseria gonorrhoeae strain FA1090

45.283

100

0.507
  comP Acinetobacter baylyi ADP1

43.506

100

0.472
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.043

100

0.451
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.836

100

0.451
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.437
  pilA Ralstonia pseudosolanacearum GMI1000

36.196

100

0.415
  pilA Pseudomonas aeruginosa PAK

34.81

100

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.735

100

0.38