Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KHW14_RS22540 Genome accession   NZ_CP074412
Coordinates   5159948..5161165 (+) Length   405 a.a.
NCBI ID   WP_043304826.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain U625     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5154948..5166165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW14_RS22515 (KHW14_22515) - 5155560..5155991 (+) 432 WP_004887456.1 hypothetical protein -
  KHW14_RS22520 (KHW14_22520) - 5156047..5157027 (+) 981 WP_213673917.1 YiiG family protein -
  KHW14_RS22530 (KHW14_22530) pilA2 5157591..5158019 (-) 429 WP_043304828.1 pilin Machinery gene
  KHW14_RS22535 (KHW14_22535) pilB 5158245..5159945 (+) 1701 WP_096767277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW14_RS22540 (KHW14_22540) pilC 5159948..5161165 (+) 1218 WP_043304826.1 type II secretion system F family protein Machinery gene
  KHW14_RS22545 (KHW14_22545) pilD 5161167..5162039 (+) 873 WP_096767275.1 A24 family peptidase Machinery gene
  KHW14_RS22550 (KHW14_22550) coaE 5162036..5162659 (+) 624 WP_025993968.1 dephospho-CoA kinase -
  KHW14_RS22555 (KHW14_22555) yacG 5162656..5162865 (+) 210 WP_004887444.1 DNA gyrase inhibitor YacG -
  KHW14_RS22560 (KHW14_22560) - 5163265..5163726 (-) 462 WP_162868424.1 hypothetical protein -
  KHW14_RS22565 (KHW14_22565) - 5163851..5164480 (+) 630 WP_004884092.1 DUF1780 domain-containing protein -
  KHW14_RS22570 (KHW14_22570) - 5164477..5165022 (+) 546 WP_043305100.1 MOSC domain-containing protein -
  KHW14_RS22575 (KHW14_22575) - 5165036..5165209 (+) 174 WP_004884089.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43947.71 Da        Isoelectric Point: 10.1763

>NTDB_id=564750 KHW14_RS22540 WP_043304826.1 5159948..5161165(+) (pilC) [Pseudomonas viridiflava strain U625]
MASKAVKVSVFTWEGVDKKGSKISGELSGHNPALIKAQLRKQGINPTKVRKKTVSIFGKGKKIKPLDIAFFARQMATMMK
AGVPLLQSFDIISEGAENPNMRSLVDSLKQEVSAGNSFATALRQKPDQFDNLFCNLVDAGEQAGALESLLDRVATYKEKT
ERLKAKIKKAMTYPAAVLMVAVVVSGILLLKVVPQFQTVFQGFGADLPAFTLMVIGLSEVVQNWWLAIIGIFVGSFFIFK
RSYKQSQKFRDSVDRFLLKVPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVMKIKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFQ
LGGAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=564750 KHW14_RS22540 WP_043304826.1 5159948..5161165(+) (pilC) [Pseudomonas viridiflava strain U625]
ATGGCCAGTAAAGCAGTCAAAGTCAGTGTTTTCACCTGGGAAGGCGTAGACAAGAAAGGCAGCAAGATCAGCGGCGAGTT
GAGTGGTCACAATCCGGCGCTGATCAAGGCTCAACTTCGTAAGCAAGGCATCAACCCGACCAAAGTGCGGAAGAAAACGG
TCTCGATCTTCGGCAAGGGTAAAAAGATCAAGCCGCTGGACATCGCTTTTTTTGCTCGGCAGATGGCGACCATGATGAAA
GCGGGCGTTCCGCTTTTACAATCTTTCGACATTATCAGCGAGGGAGCGGAGAACCCGAACATGCGCTCGCTCGTGGATTC
TCTTAAACAGGAAGTATCCGCTGGTAACAGTTTTGCAACGGCGTTAAGGCAAAAGCCCGACCAGTTCGACAATTTGTTCT
GCAATCTGGTGGACGCAGGCGAGCAGGCAGGTGCTCTAGAGAGCCTGCTGGACCGGGTGGCAACTTACAAGGAAAAAACC
GAAAGGCTCAAAGCCAAAATCAAGAAAGCCATGACTTATCCCGCCGCAGTTTTGATGGTAGCGGTCGTTGTCTCGGGCAT
TCTGCTTTTAAAAGTAGTACCACAGTTTCAAACGGTTTTTCAGGGATTCGGTGCCGACCTGCCTGCCTTTACACTAATGG
TAATCGGTCTTTCAGAAGTGGTACAAAACTGGTGGTTAGCCATTATCGGGATATTCGTCGGCAGCTTTTTCATATTCAAG
CGAAGCTACAAACAGTCACAGAAATTTCGCGACAGTGTTGACAGGTTTCTGTTGAAGGTCCCGCTGATTGGTCCGTTGAT
CTTCAAGTCATCGGTCGCTCGCTATGCTCGCACGCTGGCCACGACCTTTGCGGCGGGTGTGCCCTTGGTCGAAGCACTGG
ATTCCGTGGCTGGGGCTACAGGTAACGTCGTGTTCAGAAATGCGGTGATGAAAATCAAGCAAGATGTGTCCACGGGGATG
CAGCTGAATTTCTCAATGCGATCCACCGGCGTATTTCCAAGCCTTGCTATCCAGATGACTGCAATCGGTGAAGAGTCTGG
TGCCTTGGATAGCATGCTCGATAAGGTCGCAACCTATTACGAAGATGAAGTCGATAACATGGTCGACAGCCTGACAAGCC
TTATGGAGCCGATGATCATGGCCGTGCTGGGCGTAGTCGTCGGAGGGCTGGTCATTGCCATGTACTTGCCGATTTTCCAG
TTGGGAGGCGCGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.815

100

0.748
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

60.049

100

0.605
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilC Vibrio campbellii strain DS40M4

42.015

100

0.422
  pilG Neisseria gonorrhoeae MS11

41.832

99.753

0.417
  pilG Neisseria meningitidis 44/76-A

41.832

99.753

0.417
  pilC Vibrio cholerae strain A1552

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

37.531

100

0.375