Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHW14_RS22535 Genome accession   NZ_CP074412
Coordinates   5158245..5159945 (+) Length   566 a.a.
NCBI ID   WP_096767277.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain U625     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5153245..5164945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW14_RS22505 (KHW14_22505) - 5153656..5154654 (-) 999 WP_122407783.1 ATP-grasp domain-containing protein -
  KHW14_RS22510 (KHW14_22510) - 5154774..5155325 (+) 552 WP_029243841.1 DUF1523 family protein -
  KHW14_RS22515 (KHW14_22515) - 5155560..5155991 (+) 432 WP_004887456.1 hypothetical protein -
  KHW14_RS22520 (KHW14_22520) - 5156047..5157027 (+) 981 WP_213673917.1 YiiG family protein -
  KHW14_RS22530 (KHW14_22530) pilA2 5157591..5158019 (-) 429 WP_043304828.1 pilin Machinery gene
  KHW14_RS22535 (KHW14_22535) pilB 5158245..5159945 (+) 1701 WP_096767277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHW14_RS22540 (KHW14_22540) pilC 5159948..5161165 (+) 1218 WP_043304826.1 type II secretion system F family protein Machinery gene
  KHW14_RS22545 (KHW14_22545) pilD 5161167..5162039 (+) 873 WP_096767275.1 A24 family peptidase Machinery gene
  KHW14_RS22550 (KHW14_22550) coaE 5162036..5162659 (+) 624 WP_025993968.1 dephospho-CoA kinase -
  KHW14_RS22555 (KHW14_22555) yacG 5162656..5162865 (+) 210 WP_004887444.1 DNA gyrase inhibitor YacG -
  KHW14_RS22560 (KHW14_22560) - 5163265..5163726 (-) 462 WP_162868424.1 hypothetical protein -
  KHW14_RS22565 (KHW14_22565) - 5163851..5164480 (+) 630 WP_004884092.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62720.88 Da        Isoelectric Point: 6.3861

>NTDB_id=564749 KHW14_RS22535 WP_096767277.1 5158245..5159945(+) (pilB) [Pseudomonas viridiflava strain U625]
MSDVVVLTGLAKQLVVAELLSEKAAQQAFQQARRDKISLVSYLVQNKLVKSLTLAEMASEQFGVPFMDLSSLDKENQPKG
LVSEKLARQHHALPLWRRGNRLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDSDSGLGSLADIDLDL
DIEPGESAKETSITSRDDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKVFRVRFRTDGILHEAARPPINLANRIAARL
KVMASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEVDVPHETLIKEGFPE
SKVGTFKLYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=564749 KHW14_RS22535 WP_096767277.1 5158245..5159945(+) (pilB) [Pseudomonas viridiflava strain U625]
ATGAGTGATGTCGTTGTCCTAACCGGCCTGGCCAAGCAATTGGTCGTCGCCGAGCTTCTTTCCGAAAAAGCCGCTCAACA
GGCTTTCCAGCAGGCGCGACGCGACAAGATTTCGCTGGTCAGCTATTTGGTCCAGAACAAACTGGTCAAGAGTCTGACTC
TCGCAGAAATGGCCTCCGAGCAGTTCGGTGTGCCATTCATGGACCTCTCCAGCCTGGATAAAGAAAACCAGCCCAAAGGG
CTGGTAAGCGAAAAGCTCGCTCGTCAGCATCATGCGTTGCCGCTATGGCGTCGAGGCAACCGGCTCTTTATTGGCATCTC
GGACCCGACCAACCATCAGGCTGTGACCGATATTCAGTTCAGCACTGGCCTGAACACTGAAGCGATCCTGGTCGAAGACG
ACAAACTTACTATTGCCATCGACAGGTTCTTCGACAGCGATAGCGGCTTGGGATCATTGGCTGACATCGACCTCGATCTG
GACATAGAACCGGGCGAAAGCGCCAAAGAAACATCCATTACCAGCCGCGACGATGCCGATGACGCTCCAGTCGTACGCTT
CGTCAATAAAATGCTGCTGGATGCCATTCGGCTGGGCTCGTCCGACCTGCACTTCGAACCCTATGAAAAGGTGTTCCGCG
TGCGCTTCCGTACTGATGGGATCTTGCACGAAGCGGCGAGACCACCGATCAATCTGGCCAACCGCATTGCAGCGCGTCTG
AAGGTCATGGCCAGCCTGGATATCTCTGAGCGGCGCAAACCTCAGGACGGCCGAGTAAAATTGCGTGTTTCCAAAACAAA
ATCCATCGACTTCCGGATGAACACGCTACCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGCG
CCCAGATGGGCATTGATGCTTTAGGCTACGAGCCGGATCAGAAAGAGCTTTATCTGGAAGCGCTCAAGCAGCCGCAGGGC
ATGATTCTGGTGACCGGCCCGACCGGCTCGGGCAAAACCGTTTCGCTGTACACGGGCCTTAATATTCTCAATACGGTGAA
TATCAATATTTCAACCGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCGTCAATCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGTGAGATTCGTGACCTG
GAAACAGCCGAGATCGCGATCAAGGCCTCACAGACCGGCCACATGGTGCTGTCGACGCTGCACACCAACAGCGCAGCAGA
AACCCTCACACGCCTGCATCACATGGGCGTTGCGGCGTTCAACATCGCCACCGCTATCAACCTCATCATCGCCCAACGAC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAAGAAGTCGACGTACCCCATGAGACGCTGATCAAGGAAGGCTTTCCGGAG
TCGAAAGTCGGCACCTTCAAACTGTACGGCCCGGTTGGGTGCGAACACTGCAACGGGGGTTATCGAGGCCGAGTCGGCAT
TTACGAGGTCGTGAAAAAAACGCCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGA
TGCGCAAGGATGGTTTCAATGACCTGCGCACATCCGGCTTGTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.549

99.47

0.572
  pilB Acinetobacter baylyi ADP1

56.762

99.293

0.564
  pilB Legionella pneumophila strain ERS1305867

54.386

100

0.548
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

48.49

99.47

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.636

97.173

0.482
  pilF Neisseria gonorrhoeae MS11

47.78

99.47

0.475
  pilF Thermus thermophilus HB27

38.624

100

0.387