Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UWK_RS16420 Genome accession   NC_020304
Coordinates   3685537..3686619 (-) Length   360 a.a.
NCBI ID   WP_015405521.1    Uniprot ID   M1PE28
Organism   Desulfocapsa sulfexigens DSM 10523     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3680537..3691619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UWK_RS16405 (UWK_03307) - 3681339..3683846 (+) 2508 WP_015405518.1 DNA internalization-related competence protein ComEC/Rec2 -
  UWK_RS16410 (UWK_03308) - 3683890..3684195 (-) 306 WP_015405519.1 DUF3144 domain-containing protein -
  UWK_RS16415 (UWK_03309) - 3684227..3685507 (-) 1281 WP_015405520.1 MFS transporter -
  UWK_RS16420 (UWK_03310) pilT 3685537..3686619 (-) 1083 WP_015405521.1 type IV pilus twitching motility protein PilT Machinery gene
  UWK_RS16425 (UWK_03311) - 3686692..3687375 (-) 684 WP_015405522.1 TIGR00730 family Rossman fold protein -
  UWK_RS16430 (UWK_03312) - 3687393..3689540 (-) 2148 WP_015405523.1 tetratricopeptide repeat protein -
  UWK_RS16435 (UWK_03313) selA 3689540..3690952 (-) 1413 WP_041916456.1 L-seryl-tRNA(Sec) selenium transferase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40510.98 Da        Isoelectric Point: 7.7226

>NTDB_id=56247 UWK_RS16420 WP_015405521.1 3685537..3686619(-) (pilT) [Desulfocapsa sulfexigens DSM 10523]
MAQIDAFFKLMNDEGASDLHMVAEQQPILRIRGDMERVKFKKMGNEELKAMLYEICPEDKIKIFEELGDVDFGYEIPGLA
RYRCNYFRQKYGIGAVFREIPSEILTCEQLGLPKVISRLAYLPKGLVLVTGPTGSGKSTTLAAVIDEINRNRKDHILTIE
DPIEFVHRSQKCVINHREVGQHTKSFSAALRGALREDPDVIMIGEMRDLETISLAMEAAMTGHLVFGTLHTLNAMKTVDR
VVEIFPANQQGQVRSTLSDALKAVVSQTLFKRIDVKGRCAALEVLIATPAVRNLIREGKTYQIASVMQTGKKYGMRTLDD
SIMEYLEKRQISADDAYSNSVEKAKFLKFLKKPPSDFTEV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=56247 UWK_RS16420 WP_015405521.1 3685537..3686619(-) (pilT) [Desulfocapsa sulfexigens DSM 10523]
ATGGCTCAGATTGACGCGTTTTTTAAGTTGATGAACGATGAAGGTGCCTCGGATTTACATATGGTTGCCGAACAGCAGCC
TATTCTGCGAATTCGTGGTGACATGGAACGTGTCAAATTTAAGAAGATGGGAAACGAAGAATTGAAGGCCATGCTCTACG
AGATCTGTCCGGAAGATAAGATCAAGATTTTTGAAGAACTTGGCGATGTGGACTTTGGTTATGAAATACCTGGTCTTGCA
CGTTATCGTTGTAATTATTTCCGTCAGAAATATGGAATTGGTGCTGTCTTTCGTGAAATTCCGAGTGAGATTCTGACCTG
TGAACAGCTGGGACTGCCCAAGGTTATTTCTCGTCTTGCCTATCTTCCTAAAGGGCTGGTGCTGGTTACTGGCCCTACAG
GGTCAGGTAAATCCACAACACTGGCAGCAGTAATTGATGAAATAAACAGAAATAGAAAAGATCATATCCTGACAATTGAG
GATCCTATCGAATTTGTTCATCGTAGTCAAAAGTGTGTTATTAATCATCGCGAAGTCGGGCAGCACACCAAGAGCTTCTC
GGCAGCACTTCGTGGAGCATTGCGTGAAGATCCTGATGTCATAATGATTGGTGAGATGCGTGATCTCGAAACCATCTCTC
TTGCCATGGAAGCAGCTATGACAGGCCATCTTGTCTTTGGAACCCTCCATACACTGAATGCCATGAAAACCGTTGATCGT
GTGGTGGAGATTTTTCCTGCAAACCAGCAGGGGCAGGTTAGGTCAACACTCTCAGATGCCTTGAAGGCCGTTGTCTCCCA
GACCTTATTCAAGCGAATAGATGTAAAGGGGCGTTGTGCTGCCCTGGAAGTGCTTATAGCAACTCCTGCGGTACGAAACC
TTATCAGAGAAGGGAAAACCTATCAGATTGCCTCAGTTATGCAGACGGGTAAGAAGTATGGAATGCGTACACTCGATGAC
AGTATCATGGAATATCTGGAAAAACGTCAGATCAGTGCTGATGATGCGTATTCAAACTCAGTTGAAAAAGCAAAATTCTT
GAAATTCCTGAAGAAGCCACCGTCTGATTTTACAGAAGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1PE28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.955

92.5

0.508
  pilT Pseudomonas stutzeri DSM 10701

54.354

92.5

0.503
  pilT Neisseria gonorrhoeae MS11

52.695

92.778

0.489
  pilT Legionella pneumophila strain Lp02

53.172

91.944

0.489
  pilT Legionella pneumophila strain ERS1305867

53.172

91.944

0.489
  pilT Neisseria meningitidis 8013

54.517

89.167

0.486
  pilT Acinetobacter baumannii strain A118

52.252

92.5

0.483
  pilT Acinetobacter nosocomialis M2

52.252

92.5

0.483
  pilT Acinetobacter baumannii D1279779

52.252

92.5

0.483
  pilT Acinetobacter baylyi ADP1

55.272

86.944

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.821

96.111

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.846

90.278

0.45
  pilT Vibrio cholerae strain A1552

49.846

90.278

0.45
  pilU Pseudomonas stutzeri DSM 10701

40.12

92.778

0.372

Multiple sequence alignment