Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J5226_RS09125 Genome accession   NZ_CP072597
Coordinates   2117765..2118880 (-) Length   371 a.a.
NCBI ID   WP_215839603.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2112765..2123880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS09105 (J5226_09105) - 2113316..2115292 (-) 1977 WP_215839599.1 peptidoglycan DD-metalloendopeptidase family protein -
  J5226_RS09110 (J5226_09110) - 2115410..2115679 (+) 270 WP_215839600.1 hypothetical protein -
  J5226_RS09115 (J5226_09115) - 2115676..2116656 (+) 981 WP_215839601.1 fumarylacetoacetate hydrolase family protein -
  J5226_RS09120 (J5226_09120) maiA 2116908..2117570 (+) 663 WP_215839602.1 maleylacetoacetate isomerase -
  J5226_RS09125 (J5226_09125) pilU 2117765..2118880 (-) 1116 WP_215839603.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J5226_RS09130 (J5226_09130) - 2118983..2119360 (+) 378 WP_215839604.1 DUF4398 domain-containing protein -
  J5226_RS09135 (J5226_09135) - 2119378..2119986 (+) 609 WP_215839605.1 hypothetical protein -
  J5226_RS09140 (J5226_09140) - 2120632..2120853 (+) 222 WP_215839606.1 YdcH family protein -
  J5226_RS09145 (J5226_09145) - 2121385..2122755 (+) 1371 WP_215839607.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41113.37 Da        Isoelectric Point: 6.1912

>NTDB_id=554458 J5226_RS09125 WP_215839603.1 2117765..2118880(-) (pilU) [Lysobacter sp. K5869]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKSIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGTDGRRIPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGRIEMEEALKAADSREGLSLKFRLSEGGTGEHDPYADMYDASATH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=554458 J5226_RS09125 WP_215839603.1 2117765..2118880(-) (pilU) [Lysobacter sp. K5869]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTTTTGACCACCGGCGCGCCGGTCTA
CATCAAGGTCGAGGGTCGACTGTACCCGCTCGGCAACACCGGTTTGCCGCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAAGTTCCGCAGTTCGAGCGTGACCTCGAACTGAACATGGCCCTGGCCCTGCCCGACGCCGGCCGT
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGAGCATCATCATGGCCCCGCGCGGTCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGATCACCGCAACAGCAACACCGCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAAAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCACCACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGCCATCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTCGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTCAAGGCGGTGGTGTCGCAGCG
CTTGGTGGTCGGCACCGACGGCCGCCGCATCCCGGCCGCGGAAGTGCTGATCAACACCCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCCGGATCGAGATGGAGGAGGCGCTCAAGGCCGCCGACTCGCGCGAGGGCCTGTCGCTGAA
GTTCCGCCTCTCCGAGGGCGGCACCGGCGAACACGATCCCTACGCGGACATGTACGACGCGTCGGCGACGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Acinetobacter baylyi ADP1

49.153

95.418

0.469
  pilU Vibrio cholerae strain A1552

48.305

95.418

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.64

92.722

0.423
  pilT Acinetobacter nosocomialis M2

40.698

92.722

0.377
  pilT Acinetobacter baumannii D1279779

40.698

92.722

0.377
  pilT Acinetobacter baumannii strain A118

40.698

92.722

0.377
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baylyi ADP1

40.407

92.722

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.116

92.722

0.372
  pilT Legionella pneumophila strain Lp02

40.237

91.105

0.367
  pilT Legionella pneumophila strain ERS1305867

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

39.193

93.531

0.367
  pilT Neisseria meningitidis 8013

39.193

93.531

0.367