Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   J7445_RS12050 Genome accession   NZ_CP072524
Coordinates   2555338..2555796 (+) Length   152 a.a.
NCBI ID   WP_209283078.1    Uniprot ID   -
Organism   Neisseria sicca strain NS20201025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2550338..2560796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS12035 (J7445_12035) asd 2550710..2551825 (+) 1116 WP_070654758.1 aspartate-semialdehyde dehydrogenase -
  J7445_RS12040 (J7445_12040) recG 2552174..2554216 (+) 2043 WP_141752018.1 ATP-dependent DNA helicase RecG Machinery gene
  J7445_RS12045 (J7445_12045) - 2554515..2554625 (+) 111 Protein_2330 IS5/IS1182 family transposase -
  J7445_RS12050 (J7445_12050) pilE 2555338..2555796 (+) 459 WP_209283078.1 pilin Machinery gene
  J7445_RS12055 (J7445_12055) - 2555892..2556371 (+) 480 WP_070654762.1 pilin -
  J7445_RS12060 (J7445_12060) tyrS 2556715..2558010 (-) 1296 WP_209283079.1 tyrosine--tRNA ligase -
  J7445_RS12065 (J7445_12065) - 2558072..2559718 (-) 1647 WP_244969487.1 acyltransferase family protein -
  J7445_RS12315 - 2559750..2559893 (-) 144 WP_244969488.1 acyltransferase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16085.58 Da        Isoelectric Point: 9.3472

>NTDB_id=553930 J7445_RS12050 WP_209283078.1 2555338..2555796(+) (pilE) [Neisseria sicca strain NS20201025]
MKAIQKGFTLIELMIVIAIIGILAVIALPAYQDYTGRAQVSEAITLMEGQKSAIVEYYADKGKWPTSNTDAGIAAATDIK
GKYVAKVEVGANGVITATMNAKDVNNDIKNKTVTLQPENKNGAAASAGSFTWKCVKGTSNAVPEKFLPSSCR

Nucleotide


Download         Length: 459 bp        

>NTDB_id=553930 J7445_RS12050 WP_209283078.1 2555338..2555796(+) (pilE) [Neisseria sicca strain NS20201025]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAATTGATGATCGTAATCGCAATTATCGGTATTCTGGCAGTTATCGC
TCTGCCTGCTTACCAAGACTACACCGGTCGCGCACAAGTTTCTGAAGCAATTACCCTGATGGAAGGTCAAAAATCTGCAA
TCGTCGAATATTACGCAGATAAAGGTAAATGGCCTACAAGCAATACTGACGCAGGTATTGCGGCTGCTACAGACATTAAA
GGTAAATATGTTGCTAAGGTTGAAGTTGGTGCAAACGGTGTGATTACCGCAACAATGAATGCTAAGGATGTCAATAATGA
CATTAAAAATAAAACAGTAACTCTTCAGCCTGAAAATAAGAATGGTGCAGCGGCTTCTGCAGGTTCATTCACTTGGAAAT
GTGTTAAAGGTACGTCCAATGCCGTTCCAGAAAAATTCCTGCCTTCTTCTTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

56.024

100

0.612
  pilE Neisseria gonorrhoeae strain FA1090

54.545

100

0.592
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.526

100

0.507
  pilA/pilA1 Eikenella corrodens VA1

41.718

100

0.447
  pilA2 Legionella pneumophila str. Paris

45.27

97.368

0.441
  pilA2 Legionella pneumophila strain ERS1305867

44.595

97.368

0.434
  comP Acinetobacter baylyi ADP1

40.994

100

0.434
  pilA Acinetobacter baumannii strain A118

40

98.684

0.395
  pilA Pseudomonas aeruginosa PAK

35.976

100

0.388
  pilA Vibrio campbellii strain DS40M4

38.816

100

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.908

100

0.382
  pilA Haemophilus influenzae Rd KW20

37.748

99.342

0.375
  pilA Ralstonia pseudosolanacearum GMI1000

32.934

100

0.362