Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B479_RS25085 Genome accession   NC_019905
Coordinates   5516876..5517874 (-) Length   332 a.a.
NCBI ID   WP_015272146.1    Uniprot ID   A0A2N5BU21
Organism   Pseudomonas putida HB3267     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5511876..5522874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B479_RS25065 (B479_24915) argS 5512944..5514680 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  B479_RS25070 (B479_24920) - 5514682..5515380 (+) 699 WP_003257831.1 SPOR domain-containing protein -
  B479_RS25075 (B479_24925) - 5515527..5515946 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  B479_RS25080 (B479_24930) - 5516113..5516748 (+) 636 WP_043935972.1 C40 family peptidase -
  B479_RS25085 (B479_24935) pilT 5516876..5517874 (-) 999 WP_015272146.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  B479_RS25090 (B479_24940) - 5517940..5518626 (+) 687 WP_015272147.1 YggS family pyridoxal phosphate-dependent enzyme -
  B479_RS25095 (B479_24945) proC 5518665..5519483 (+) 819 WP_015272148.1 pyrroline-5-carboxylate reductase -
  B479_RS25100 (B479_24950) - 5519494..5520084 (+) 591 WP_013974619.1 YggT family protein -
  B479_RS25105 (B479_24955) - 5520184..5521323 (+) 1140 WP_013974620.1 homoserine O-acetyltransferase -
  B479_RS25110 (B479_24960) metW 5521331..5521951 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  B479_RS25115 (B479_24965) - 5521974..5522408 (+) 435 WP_013974622.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35814.46 Da        Isoelectric Point: 6.6988

>NTDB_id=55378 B479_RS25085 WP_015272146.1 5516876..5517874(-) (pilT) [Pseudomonas putida HB3267]
MDVTDLLARAVDAGASDLHLAAGQLPMLRLDGELQRMALPTLGPATLTKGMAPLLDEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLVDVFQAVAQCRDGLILVGGPTGSGKSSTLAALLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSVDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTSAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=55378 B479_RS25085 WP_015272146.1 5516876..5517874(-) (pilT) [Pseudomonas putida HB3267]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGTTACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCGGCGACCTTGACCAAGGGCATGGCACCCT
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGCGCTAGGGCGC
TTTCGGCTGAATCTGTTTCGCCAGTTGCACGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATAGCCACGCTCGA
TGAACTCGACCTGGTCGATGTGTTTCAAGCTGTTGCGCAGTGCCGAGATGGCTTGATCCTCGTCGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCTTGGCGGCACTGCTCGACCAACTGAACCGAGATCGTGCTCTGCATATCATCACCCTTGAGGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGTGCGGCTGAGACCGGGCACCTGGTACTGGCGACCGTGCATACCCGTTCGGCGGTGAGCAGTGTTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAACCGTTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGGT
GCTGGTCAGGCGCGCGGGCGGAGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGTCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGAATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGAGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N5BU21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.818

99.398

0.515
  pilT Acinetobacter nosocomialis M2

50.909

99.398

0.506
  pilT Acinetobacter baumannii D1279779

50.909

99.398

0.506
  pilT Acinetobacter baumannii strain A118

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.606

99.398

0.503
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Legionella pneumophila strain Lp02

49.091

99.398

0.488
  pilT Legionella pneumophila strain ERS1305867

49.091

99.398

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria meningitidis 8013

48.64

99.699

0.485
  pilT Neisseria gonorrhoeae MS11

48.338

99.699

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.619

99.699

0.455
  pilU Pseudomonas stutzeri DSM 10701

36.858

99.699

0.367

Multiple sequence alignment