Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CYAN10605_RS05500 Genome accession   NC_019776
Coordinates   1328329..1329441 (+) Length   370 a.a.
NCBI ID   WP_015218946.1    Uniprot ID   A0AAF0ZK70
Organism   Cyanobacterium aponinum PCC 10605     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1323329..1334441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYAN10605_RS05475 (Cyan10605_1088) - 1323659..1324939 (-) 1281 WP_015218942.1 Glu/Leu/Phe/Val dehydrogenase -
  CYAN10605_RS05480 (Cyan10605_1090) - 1325874..1326233 (+) 360 WP_015218943.1 DUF3593 domain-containing protein -
  CYAN10605_RS05485 (Cyan10605_1091) - 1326486..1327313 (-) 828 WP_015218944.1 urease accessory protein UreD -
  CYAN10605_RS05490 (Cyan10605_1092) - 1327548..1328018 (-) 471 WP_015218945.1 CYTH domain-containing protein -
  CYAN10605_RS05500 (Cyan10605_1093) pilT 1328329..1329441 (+) 1113 WP_015218946.1 type IV pilus twitching motility protein PilT Machinery gene
  CYAN10605_RS05505 (Cyan10605_1094) - 1329473..1330687 (+) 1215 WP_015218947.1 type II secretion system F family protein -
  CYAN10605_RS05510 (Cyan10605_1095) rplU 1330871..1331254 (+) 384 WP_015218948.1 50S ribosomal protein L21 -
  CYAN10605_RS05515 (Cyan10605_1096) rpmA 1331320..1331574 (+) 255 WP_015218949.1 50S ribosomal protein L27 -
  CYAN10605_RS05520 (Cyan10605_1097) - 1331734..1332462 (-) 729 WP_015218950.1 Bax inhibitor-1 family protein -
  CYAN10605_RS05525 (Cyan10605_1098) - 1332493..1333401 (-) 909 WP_015218951.1 PhzF family phenazine biosynthesis protein -
  CYAN10605_RS05530 (Cyan10605_1099) - 1333609..1334184 (+) 576 WP_015218952.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40552.89 Da        Isoelectric Point: 6.6491

>NTDB_id=55030 CYAN10605_RS05500 WP_015218946.1 1328329..1329441(+) (pilT) [Cyanobacterium aponinum PCC 10605]
MEIMIEDLMEQLVELGGSDMHIQAGAPVYFRISGKLTPIGDEPLNAQECQKLIFSMLNNTQRKTLEQEWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDQLGLPDIVKEMSERPRGMILVTGQTGSGKTTTLAAMLDLINRTRAEHILTV
EDPIEYVFPNINSLFHQRQKGEDTKSFANALKAALREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAGTID
RIIDVFPSAEQAQIRAMLSNSLLAVFAQTLAKKKNPKPGEFGRAMAQEIMIVTPAIANLIREAKAPQIYSAIQTGGKLGM
QTMEQALANLVKTGTIAVEEALAKSSKPDELKRLLGSSMDSGMVTPSKRR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=55030 CYAN10605_RS05500 WP_015218946.1 1328329..1329441(+) (pilT) [Cyanobacterium aponinum PCC 10605]
ATGGAGATAATGATTGAAGATTTGATGGAGCAGTTAGTAGAGTTAGGGGGGTCAGATATGCACATTCAGGCAGGAGCCCC
CGTCTATTTCCGTATTAGCGGTAAACTAACCCCCATTGGAGATGAACCCCTTAATGCTCAAGAGTGCCAAAAATTAATTT
TTAGTATGCTCAATAATACTCAACGTAAAACATTAGAGCAAGAATGGGAGTTAGACTGTTCCTACGGGGTAAAAGGCTTG
GCTCGATTTCGTGTAAATGTTTATAAGGAAAGGGGTTGTTATGCCGCTTGTTTAAGGGCTTTATCTTCCAAAATTCCCAA
TTTTGATCAATTAGGCTTACCAGATATTGTCAAGGAGATGTCCGAACGCCCTAGGGGTATGATTTTAGTGACGGGGCAGA
CAGGTTCTGGAAAAACTACTACTTTAGCGGCAATGTTGGACTTAATTAACCGCACTCGTGCAGAACACATCTTAACAGTA
GAAGATCCGATCGAGTATGTTTTTCCCAATATTAACAGTCTATTTCACCAACGTCAAAAAGGAGAAGATACTAAAAGTTT
TGCCAATGCCTTAAAAGCGGCGTTAAGGGAAGATCCAGATATTATTCTAGTGGGTGAGATGCGCGACTTAGAAACTATTT
CCTTGGCGATTACGGCGGCGGAAACTGGACACCTTGTCTTTGGTACTTTACATACTAGCTCTGCGGCGGGAACAATTGAT
CGTATTATTGATGTATTTCCTTCGGCAGAACAAGCCCAAATTAGAGCAATGTTATCTAACTCTTTATTGGCAGTATTTGC
TCAAACTTTGGCTAAAAAGAAAAATCCCAAACCGGGCGAATTTGGTAGGGCAATGGCTCAAGAAATCATGATTGTGACAC
CTGCGATCGCAAACTTAATTAGAGAAGCAAAAGCCCCACAAATTTATTCTGCGATTCAAACAGGGGGTAAATTAGGAATG
CAAACCATGGAACAGGCTTTAGCAAATTTGGTGAAAACAGGTACAATTGCAGTGGAAGAAGCCCTTGCCAAGAGCAGTAA
ACCAGATGAATTGAAACGCTTATTAGGTTCTTCTATGGATAGCGGGATGGTTACTCCTAGCAAACGTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.623

93.243

0.5
  pilT Legionella pneumophila strain Lp02

53.623

93.243

0.5
  pilT Pseudomonas aeruginosa PAK

52.45

93.784

0.492
  pilT Acinetobacter nosocomialis M2

51.585

93.784

0.484
  pilT Acinetobacter baumannii D1279779

51.585

93.784

0.484
  pilT Acinetobacter baumannii strain A118

51.585

93.784

0.484
  pilT Acinetobacter baylyi ADP1

51.297

93.784

0.481
  pilT Pseudomonas stutzeri DSM 10701

50.144

93.784

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.44

92.162

0.465
  pilT Vibrio cholerae strain A1552

50.44

92.162

0.465
  pilT Neisseria gonorrhoeae MS11

48.138

94.324

0.454
  pilT Neisseria meningitidis 8013

48.415

93.784

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.727

95.135

0.454
  pilU Pseudomonas stutzeri DSM 10701

37.741

98.108

0.37

Multiple sequence alignment