Detailed information NTG0549720

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: LSG40_RS26500
• Genome accession: NZ_CP089236
• Coordinates: 5472551..5473003 (-)
• Length: 150 a.a.
• NCBI ID: WP_114064701.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain JNQH-PA027
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5467551..5478003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSG40_RS26465 (LSG40_26465)	-	5468007..5468669 (+)	663	WP_033992423.1	DUF3218 family protein	-
LSG40_RS26470 (LSG40_26470)	fliP	5468952..5469704 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
LSG40_RS26475 (LSG40_26475)	fliQ	5469749..5470018 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
LSG40_RS26480 (LSG40_26480)	fliR	5470018..5470794 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
LSG40_RS26485 (LSG40_26485)	-	5470797..5471057 (+)	261	Protein_5258	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
LSG40_RS26490 (LSG40_26490)	-	5472054..5472296 (+)	243	WP_023082027.1	hypothetical protein	-
LSG40_RS26500 (LSG40_26500)	pilA	5472551..5473003 (-)	453	WP_114064701.1	pilin	Machinery gene
LSG40_RS26505 (LSG40_26505)	pilB	5473235..5474935 (+)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LSG40_RS26510 (LSG40_26510)	pilC	5474939..5476159 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
LSG40_RS26515 (LSG40_26515)	pilD	5476163..5477035 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
LSG40_RS26520 (LSG40_26520)	coaE	5477032..5477643 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
LSG40_RS26525 (LSG40_26525)	yacG	5477640..5477840 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15471.67 Da
• Isoelectric Point: 7.7827

>NTDB_id=549720 LSG40_RS26500 WP_114064701.1 5472551..5473003(-) (pilA) [Pseudomonas aeruginosa strain JNQH-PA027]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTEDTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

• Length: 453 bp

>NTDB_id=549720 LSG40_RS26500 WP_114064701.1 5472551..5473003(-) (pilA) [Pseudomonas aeruginosa strain JNQH-PA027]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGAAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	68.456	99.333	0.68
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.333	100	0.473
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Vibrio parahaemolyticus RIMD 2210633	46.259	98	0.453
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
pilA2	Legionella pneumophila strain ERS1305867	42.282	99.333	0.42
comP	Acinetobacter baylyi ADP1	40.909	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
pilA2	Legionella pneumophila str. Paris	41.611	99.333	0.413