Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIC7113_RS04205 Genome accession   NC_019738
Coordinates   989824..990960 (-) Length   378 a.a.
NCBI ID   WP_041780443.1    Uniprot ID   -
Organism   Allocoleopsis franciscana PCC 7113     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 984824..995960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIC7113_RS04175 (Mic7113_0858) - 985047..985499 (-) 453 WP_015180920.1 ferredoxin -
  MIC7113_RS04180 (Mic7113_0859) - 985499..985885 (-) 387 WP_015180921.1 DUF1257 domain-containing protein -
  MIC7113_RS04185 (Mic7113_0860) - 985927..986142 (-) 216 WP_015180922.1 DUF2997 domain-containing protein -
  MIC7113_RS04190 (Mic7113_0861) - 986505..987545 (+) 1041 WP_015180923.1 saccharopine dehydrogenase-like oxidoreductase -
  MIC7113_RS04195 (Mic7113_0862) - 987625..988215 (-) 591 WP_015180924.1 hypothetical protein -
  MIC7113_RS04200 (Mic7113_0863) - 988409..989626 (-) 1218 WP_015180925.1 type II secretion system F family protein -
  MIC7113_RS04205 (Mic7113_0864) pilT 989824..990960 (-) 1137 WP_041780443.1 type IV pilus twitching motility protein PilT Machinery gene
  MIC7113_RS04210 (Mic7113_0865) pilB/pilB1 991063..993060 (-) 1998 WP_015180927.1 GspE/PulE family protein Machinery gene
  MIC7113_RS04215 (Mic7113_0866) grpE 993808..994593 (+) 786 WP_015180928.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41537.96 Da        Isoelectric Point: 7.1628

>NTDB_id=54877 MIC7113_RS04205 WP_041780443.1 989824..990960(-) (pilT) [Allocoleopsis franciscana PCC 7113]
MELMIEDLMEQLIEMGGSDMHIQSGAPVYFRISGKLQPIHEDPLTPHECQKLIFSMLNNTQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGYYAACLRALSSKIPNFDQLNLPDIVREMSERPRGLVLVTGPTGSGKTTTLAAMVDLINRTRPEHILTV
EDPIEYVFPNIKSLVHQRQKGEDTKSFANALKAALREDPDVILVGEMRDLETISLAISAAETGHLVMGTLHTSSAASTIN
RIIDVFPPEQQQQINAQLSNSLIAVFSQTLIPKKNPKPGEFGRVMGQEIMIVTPAIANLIREGKIAQIYSAIQTGSKMGM
QTMEQSLAHLVKTGQITFEAAVSKSSKPEEMQRILAGAGSAAIGNMGAKVAPGTKVAR

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=54877 MIC7113_RS04205 WP_041780443.1 989824..990960(-) (pilT) [Allocoleopsis franciscana PCC 7113]
ATGGAACTAATGATTGAAGACTTGATGGAGCAGTTGATTGAAATGGGTGGCTCAGATATGCACATTCAATCCGGTGCCCC
CGTCTACTTCCGCATTAGTGGAAAACTCCAGCCCATTCACGAAGACCCCCTGACCCCCCACGAATGTCAGAAACTGATCT
TCAGTATGCTCAATAATACCCAACGCAAGGAGTTAGAGCAGAATTGGGAATTAGATTGTTCTTATGGAGTTAAGGGATTG
GCTCGCTTCCGCGTCAATGTCTATAAAGAACGGGGTTACTATGCGGCTTGCTTGAGGGCGTTGTCTTCCAAAATCCCTAA
CTTTGACCAGTTGAATTTACCCGATATTGTTCGGGAAATGAGTGAAAGACCAAGAGGATTAGTGTTGGTGACAGGACCTA
CGGGGTCTGGTAAAACCACCACCCTGGCGGCAATGGTTGACCTGATTAATCGAACCCGACCCGAACATATTCTAACGGTT
GAAGACCCCATTGAATATGTGTTCCCCAACATTAAAAGTTTGGTTCATCAACGCCAAAAGGGTGAAGATACCAAAAGCTT
TGCTAATGCTTTGAAAGCAGCATTGCGGGAAGACCCTGATGTAATTTTGGTCGGAGAAATGCGGGATTTGGAAACCATTT
CTCTAGCCATTTCGGCAGCAGAGACAGGTCACTTGGTGATGGGAACCCTGCATACTAGTTCGGCTGCTTCGACGATCAAC
CGGATTATCGACGTGTTCCCGCCAGAGCAACAACAACAAATTAATGCTCAGCTTTCTAACTCCTTAATTGCCGTCTTTAG
TCAAACTTTAATACCCAAGAAAAATCCAAAACCGGGTGAATTTGGTCGGGTGATGGGGCAGGAAATCATGATTGTAACCC
CTGCGATCGCAAACTTGATCCGAGAAGGGAAAATAGCTCAAATTTACTCTGCCATCCAAACCGGTTCAAAAATGGGGATG
CAGACGATGGAACAGTCGTTAGCGCATTTGGTTAAAACAGGTCAAATTACGTTTGAAGCGGCTGTATCTAAGAGTTCCAA
ACCTGAAGAGATGCAGCGGATTCTGGCCGGTGCTGGAAGTGCGGCTATCGGAAATATGGGGGCCAAGGTTGCTCCAGGGA
CTAAAGTAGCTCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.333

91.27

0.487
  pilT Legionella pneumophila strain Lp02

53.333

91.27

0.487
  pilT Pseudomonas aeruginosa PAK

51.304

91.27

0.468
  pilT Acinetobacter baumannii strain A118

51.014

91.27

0.466
  pilT Acinetobacter nosocomialis M2

51.014

91.27

0.466
  pilT Acinetobacter baumannii D1279779

51.014

91.27

0.466
  pilT Acinetobacter baylyi ADP1

51.014

91.27

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.554

97.354

0.463
  pilT Pseudomonas stutzeri DSM 10701

50.145

91.27

0.458
  pilT Neisseria gonorrhoeae MS11

49.284

92.328

0.455
  pilT Neisseria meningitidis 8013

48.997

92.328

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.706

89.947

0.447
  pilT Vibrio cholerae strain A1552

49.706

89.947

0.447

Multiple sequence alignment