Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MIC7113_RS04210 Genome accession   NC_019738
Coordinates   991063..993060 (-) Length   665 a.a.
NCBI ID   WP_015180927.1    Uniprot ID   K9WB69
Organism   Allocoleopsis franciscana PCC 7113     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 986063..998060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIC7113_RS04190 (Mic7113_0861) - 986505..987545 (+) 1041 WP_015180923.1 saccharopine dehydrogenase-like oxidoreductase -
  MIC7113_RS04195 (Mic7113_0862) - 987625..988215 (-) 591 WP_015180924.1 hypothetical protein -
  MIC7113_RS04200 (Mic7113_0863) - 988409..989626 (-) 1218 WP_015180925.1 type II secretion system F family protein -
  MIC7113_RS04205 (Mic7113_0864) pilT 989824..990960 (-) 1137 WP_041780443.1 type IV pilus twitching motility protein PilT Machinery gene
  MIC7113_RS04210 (Mic7113_0865) pilB/pilB1 991063..993060 (-) 1998 WP_015180927.1 GspE/PulE family protein Machinery gene
  MIC7113_RS04215 (Mic7113_0866) grpE 993808..994593 (+) 786 WP_015180928.1 nucleotide exchange factor GrpE -
  MIC7113_RS04220 (Mic7113_0867) dnaK 994827..996797 (+) 1971 WP_015180929.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 74337.04 Da        Isoelectric Point: 4.9956

>NTDB_id=54878 MIC7113_RS04210 WP_015180927.1 991063..993060(-) (pilB/pilB1) [Allocoleopsis franciscana PCC 7113]
MTNSSSQRRALIVRNEVSPFGKSLIAAGYVDTDQMQNALVESRKSGRPLTEILESMTGRQLSPDLLRQYKKQQLFELKVL
YGVESLDPEINDIATNQVGQLIDTLLPIDICRRYRLVPLSKNETQPPSLLVAMVDPDNLDAQDDLNRILRPQGISLQRMV
ITQEDYQQIISKYLDEKLAKQKQMEVEQAVDVKQDLQNIDSLDTLSDAPDEEAELDVGGAEDAPVINLVNKVLAKALQEG
VSDIHVEPQENSLRIRFRKDGVLRQAFEPLPKKITPAVSARFKIMAELDIAERRLPQDGKIRRMYQGRKVDFRVSTLPSR
YGEKVVLRILDNEQTQLGLDKLITDQDTLQMVRDMANRPFGLILVTGPTGSGKSTTLYSMLAERNDPGINISTAEDPIEY
SLAGITQVQVLREKGMDFASILRSFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIPRLDEMGVEP
FMVSNALLGVLAQRLIRRVCSECRITYVPTSAELARFGLSTSREGEIMLYKANTLQPDEIAEARAKGKLCQKCNGVGYKG
RVGVYEVMRISDRLQTLINEAAPAERLKEAAVEEGMTTLLAYSLNLVQQGYTTLDEVERVTFTDSGLEAERKAKRKSSLS
CRTCSATLQQEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=54878 MIC7113_RS04210 WP_015180927.1 991063..993060(-) (pilB/pilB1) [Allocoleopsis franciscana PCC 7113]
ATGACTAACTCCTCATCACAACGACGTGCCCTGATCGTTCGCAACGAGGTTTCGCCCTTTGGTAAATCTTTGATTGCAGC
GGGTTACGTTGACACCGACCAGATGCAGAATGCGTTGGTGGAATCCCGCAAGTCAGGAAGACCCCTGACAGAAATTCTCG
AATCCATGACGGGGCGTCAGCTTTCCCCCGACTTACTGCGGCAATATAAGAAACAGCAGTTATTTGAGCTAAAAGTTCTT
TACGGGGTTGAATCTCTCGATCCGGAAATTAATGACATTGCTACCAATCAAGTGGGTCAACTAATTGACACACTGCTCCC
CATTGATATTTGTCGTCGCTATCGCCTAGTCCCGCTATCGAAGAATGAAACCCAACCTCCATCGCTGCTGGTGGCGATGG
TAGACCCGGATAATCTCGATGCTCAGGATGATCTTAACCGCATCTTGCGACCTCAGGGAATCTCGCTGCAAAGGATGGTC
ATTACCCAAGAAGACTATCAGCAGATCATCTCCAAGTACCTTGATGAAAAGCTGGCCAAGCAAAAGCAGATGGAGGTTGA
ACAAGCTGTAGATGTCAAACAAGACCTACAGAATATCGACAGCCTAGATACATTATCCGATGCACCGGACGAAGAAGCTG
AGCTGGATGTGGGGGGAGCAGAAGATGCTCCTGTGATTAACTTGGTTAATAAAGTTCTTGCCAAGGCACTGCAAGAGGGC
GTTTCAGATATTCACGTCGAACCGCAAGAGAATTCTTTACGCATCCGATTCCGGAAAGATGGGGTGCTGCGCCAAGCATT
CGAGCCTTTACCGAAGAAAATCACTCCAGCGGTGTCGGCTCGCTTCAAAATTATGGCAGAGCTGGATATTGCCGAACGAC
GACTGCCTCAAGACGGTAAAATTCGGCGGATGTACCAGGGTCGCAAAGTTGACTTTCGCGTTAGTACCTTGCCCAGCCGC
TATGGCGAAAAAGTTGTTCTACGAATTTTGGACAACGAGCAAACCCAACTAGGTTTGGATAAGTTAATCACTGACCAAGA
TACGCTGCAAATGGTCAGGGATATGGCAAACCGTCCGTTTGGCCTGATTTTGGTAACAGGACCCACGGGTTCGGGTAAAT
CCACAACCTTATACTCGATGCTGGCGGAACGGAATGACCCTGGAATCAATATCAGTACGGCGGAAGACCCTATTGAATAT
TCCTTAGCGGGTATTACTCAGGTGCAGGTGCTGCGCGAGAAGGGCATGGACTTTGCCTCGATTCTACGGTCTTTTTTGCG
ACAAGACCCAGACGTGATTCTGGTCGGTGAGACAAGGGATAAGGAAACCGCTAAAACGGCGATTGAAGCGGCACTCACGG
GTCACTTAGTACTCACCACGCTTCACACAAACGACGCGGCTGGTGCGATCCCACGATTAGACGAAATGGGCGTTGAACCC
TTCATGGTTTCCAATGCTCTGTTGGGCGTCTTGGCGCAACGATTGATTCGACGTGTTTGCTCCGAGTGCCGCATTACTTA
TGTGCCAACCTCTGCCGAATTAGCTCGCTTTGGTCTATCGACCTCCCGTGAAGGCGAAATCATGCTCTATAAAGCCAACA
CCCTACAACCCGATGAAATCGCCGAGGCCAGAGCTAAAGGTAAACTTTGCCAAAAGTGTAATGGTGTTGGCTACAAAGGA
CGTGTTGGTGTGTATGAAGTCATGCGTATTAGCGATCGCCTTCAAACCTTAATCAACGAAGCTGCTCCAGCCGAGCGACT
GAAGGAAGCCGCTGTAGAAGAGGGAATGACCACTTTATTAGCCTATAGCTTAAATCTGGTACAGCAAGGCTACACGACAC
TCGATGAAGTGGAACGTGTGACCTTTACCGATTCAGGGTTAGAAGCCGAACGCAAAGCCAAACGTAAGAGTTCCCTCTCT
TGTCGGACTTGCTCAGCCACATTACAACAAGAGTGGTTAGACTGTCCTTACTGCATGACCCCTCGTTTTCAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)

Type II/IV secretion system protein (T2SSE)

(231-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9WB69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

69.894

99.398

0.695
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.28

91.88

0.361

Multiple sequence alignment