Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LSP20_RS09555 Genome accession   NZ_CP089065
Coordinates   2033007..2033459 (+) Length   150 a.a.
NCBI ID   WP_023518088.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain UNC_PaerCF34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2028007..2038459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP20_RS09530 (LSP20_09530) yacG 2028170..2028370 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  LSP20_RS09535 (LSP20_09535) coaE 2028367..2028978 (-) 612 WP_023518089.1 dephospho-CoA kinase -
  LSP20_RS09540 (LSP20_09540) pilD 2028975..2029847 (-) 873 WP_009875878.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LSP20_RS09545 (LSP20_09545) pilC 2029851..2031071 (-) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  LSP20_RS09550 (LSP20_09550) pilB 2031075..2032775 (-) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSP20_RS09555 (LSP20_09555) pilA 2033007..2033459 (+) 453 WP_023518088.1 pilin Machinery gene
  LSP20_RS09565 (LSP20_09565) nadC 2033658..2034506 (-) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LSP20_RS09570 (LSP20_09570) - 2034792..2037047 (+) 2256 WP_023518087.1 DUF1631 domain-containing protein -
  LSP20_RS09575 (LSP20_09575) ampD 2037198..2037764 (+) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15686.02 Da        Isoelectric Point: 7.7814

>NTDB_id=548556 LSP20_RS09555 WP_023518088.1 2033007..2033459(+) (pilA) [Pseudomonas aeruginosa strain UNC_PaerCF34]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=548556 LSP20_RS09555 WP_023518088.1 2033007..2033459(+) (pilA) [Pseudomonas aeruginosa strain UNC_PaerCF34]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.651

99.333

0.473
  pilA Vibrio cholerae strain A1552

47.651

99.333

0.473
  pilA Vibrio cholerae C6706

47.651

99.333

0.473
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.333

100

0.453
  pilA Acinetobacter baumannii strain A118

44.595

98.667

0.44
  pilA Vibrio parahaemolyticus RIMD 2210633

43.243

98.667

0.427
  comP Acinetobacter baylyi ADP1

42

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

39.597

99.333

0.393
  pilA2 Legionella pneumophila str. Paris

38.926

99.333

0.387
  pilA/pilA1 Eikenella corrodens VA1

38.514

98.667

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

38.889

96

0.373
  pilA Acinetobacter nosocomialis M2

41.353

88.667

0.367