Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HIV01_RS09770 Genome accession   NZ_CP071517
Coordinates   2090381..2090803 (+) Length   140 a.a.
NCBI ID   WP_200606752.1    Uniprot ID   -
Organism   Lysobacter arenosi strain R7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2090962..2104331 2090381..2090803 flank 159


Gene organization within MGE regions


Location: 2090381..2104331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIV01_RS09770 (HIV01_009770) pilA 2090381..2090803 (+) 423 WP_200606752.1 pilin Machinery gene
  HIV01_RS09775 (HIV01_009775) - 2090962..2091363 (+) 402 WP_200606753.1 pilin -
  HIV01_RS09780 (HIV01_009780) - 2091439..2093343 (+) 1905 WP_200606754.1 hypothetical protein -
  HIV01_RS09785 (HIV01_009785) - 2093370..2094218 (+) 849 WP_200606755.1 hypothetical protein -
  HIV01_RS09790 (HIV01_009790) - 2094220..2095518 (+) 1299 WP_207526915.1 oligosaccharide flippase family protein -
  HIV01_RS09795 (HIV01_009795) - 2095555..2096619 (-) 1065 WP_200606757.1 glycosyltransferase family 4 protein -
  HIV01_RS09800 (HIV01_009800) - 2096623..2097711 (-) 1089 WP_200606758.1 UDP-N-acetylglucosamine 2-epimerase -
  HIV01_RS09805 (HIV01_009805) - 2097708..2098580 (-) 873 WP_200606760.1 glycosyltransferase family 2 protein -
  HIV01_RS09810 (HIV01_009810) pilB 2098753..2100477 (+) 1725 WP_200606762.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HIV01_RS09815 (HIV01_009815) pilC 2100619..2101875 (+) 1257 WP_200606764.1 type II secretion system F family protein Machinery gene
  HIV01_RS09820 (HIV01_009820) pilD 2101913..2102776 (+) 864 WP_200606766.1 A24 family peptidase Machinery gene
  HIV01_RS09825 (HIV01_009825) - 2102805..2103554 (-) 750 WP_200606767.1 class I SAM-dependent methyltransferase -
  HIV01_RS09830 (HIV01_009830) coaE 2103708..2104331 (+) 624 WP_200606768.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14156.39 Da        Isoelectric Point: 8.4815

>NTDB_id=545895 HIV01_RS09770 WP_200606752.1 2090381..2090803(+) (pilA) [Lysobacter arenosi strain R7]
MKNNQQGFTLIELMIVVAIIAILAAIAIPAYQDYVAKSQATAGLAEVAPGKTQYELMVQEGTAIANAASVGLQATTGRCD
IVVAAVAAGSAPATTDNIVCTLKGAAGKVAGKKLNLNRAASGAWTCTTDLNTKYKPGGCA

Nucleotide


Download         Length: 423 bp        

>NTDB_id=545895 HIV01_RS09770 WP_200606752.1 2090381..2090803(+) (pilA) [Lysobacter arenosi strain R7]
ATGAAGAACAATCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGCCATCCTCGCCGCTATCGC
CATCCCGGCTTACCAGGACTACGTCGCCAAGTCGCAGGCGACCGCCGGCCTGGCCGAAGTGGCCCCGGGCAAGACCCAGT
ATGAGCTGATGGTCCAGGAAGGCACGGCGATCGCCAACGCCGCCAGCGTCGGCCTGCAGGCCACCACCGGCCGCTGCGAT
ATCGTCGTTGCCGCCGTCGCTGCTGGCTCGGCTCCGGCCACCACCGACAACATCGTCTGCACCCTGAAGGGCGCGGCCGG
CAAGGTTGCGGGCAAGAAGCTCAACCTCAACCGCGCTGCCAGCGGTGCCTGGACCTGCACCACCGACCTGAACACCAAGT
ACAAGCCGGGCGGCTGCGCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50

100

0.5
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.936

100

0.493
  pilA Vibrio cholerae strain A1552

44.218

100

0.464
  pilA Vibrio cholerae C6706

44.218

100

0.464
  pilA Pseudomonas aeruginosa PAK

44.218

100

0.464
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.218

100

0.464
  pilA Ralstonia pseudosolanacearum GMI1000

38.554

100

0.457
  pilA2 Legionella pneumophila strain ERS1305867

41.447

100

0.45
  pilA2 Legionella pneumophila str. Paris

41.447

100

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

100

0.443
  comP Acinetobacter baylyi ADP1

39.735

100

0.429
  pilA/pilA1 Eikenella corrodens VA1

37.662

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

43.307

90.714

0.393
  pilA Acinetobacter nosocomialis M2

40.625

91.429

0.371