Detailed information NTG0054442

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ANA_RS15720
• Genome accession: NC_019427
• Coordinates: 3620943..3622043 (+)
• Length: 366 a.a.
• NCBI ID: WP_015081026.1
• Uniprot ID: K7W4D0
• Organism: Anabaena sp. 90
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3615943..3627043

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ANA_RS15710 (ANA_C13199)	grpE	3617731..3618405 (-)	675	WP_015081024.1	nucleotide exchange factor GrpE	-
ANA_RS15715 (ANA_C13200)	pilB/pilB1	3618878..3620878 (+)	2001	WP_015081025.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
ANA_RS15720 (ANA_C13201)	pilT	3620943..3622043 (+)	1101	WP_015081026.1	type IV pilus twitching motility protein PilT	Machinery gene
ANA_RS27135	-	3622030..3622176 (-)	147	WP_200863829.1	hypothetical protein	-
ANA_RS15725 (ANA_C13202)	-	3622208..3623431 (+)	1224	WP_015081027.1	type II secretion system F family protein	-
ANA_RS15730 (ANA_C13203)	-	3623850..3624983 (-)	1134	WP_371260351.1	saccharopine dehydrogenase-like oxidoreductase	-

Sequence

Protein

• Length: 366 a.a.
• Molecular weight: 40193.53 Da
• Isoelectric Point: 6.8435

>NTDB_id=54442 ANA_RS15720 WP_015081026.1 3620943..3622043(+) (pilT) [Anabaena sp. 90]
MEMMIEDLMEELITRGGSDLHLSAGLPAYFRISGHLTPIGEHILTADECQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPNIIMVGEMRDLETIMLAISAAETGHLVLGPLHTSSAAQTVD
RIIDVFPSEKQTQVRVQLSNSLVAVFSQTLVPKKNVNSGEYGRVMAQEIMIITPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAISKTSKPDEVERLIGATAPVGAKLR

Nucleotide

• Length: 1101 bp

>NTDB_id=54442 ANA_RS15720 WP_015081026.1 3620943..3622043(+) (pilT) [Anabaena sp. 90]
ATGGAAATGATGATTGAAGACTTGATGGAAGAACTAATTACTAGAGGTGGGTCAGACTTGCACCTATCCGCTGGATTGCC
TGCCTATTTTCGGATTAGTGGACATCTGACACCTATTGGTGAACACATATTGACGGCTGATGAATGTCAAAGGCTAATTT
TCAGTATGCTCAATAACACTCAGCGCAAAACTTTAGAGCAAACATGGGAATTAGATTGTTCTTACGGTGTTAAAGGATTA
GCTAGGTTCCGGGTGAATGTCTATAAAGAACGTGGTGCTTATGCTGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATAGTCCGGGAAATGACGGAAAAACCTAGAGGACTAATTCTCGTCACAGGTCCAA
CTGGTTCTGGTAAGACTACTACCCTAGCGGCAATGATTGACTTAATTAACCGCACTAGGGCAGAACATATTTTAACAGTT
GAAGACCCTGTGGAATTTGTTTATGAACCTATCAAAAGTCTAGTTCACCAACGCCAATTAGGAGAAGATACGAAGAGTTT
TGCTAATGCTTTAAAAGCAGCTTTACGAGAAGATCCAAATATTATTATGGTAGGAGAAATGCGGGACTTGGAAACCATTA
TGTTGGCGATTTCCGCCGCAGAAACAGGTCACTTGGTACTTGGTCCACTACATACAAGTTCTGCCGCACAAACCGTTGAC
CGGATTATTGACGTTTTTCCCTCAGAAAAACAAACTCAGGTGCGGGTGCAGTTGTCTAACTCCTTAGTCGCAGTTTTTAG
TCAGACTTTAGTACCTAAGAAAAATGTCAATTCAGGTGAATATGGGCGAGTAATGGCTCAAGAAATTATGATTATCACTC
CCGCTATTTCTAACTTAATTCGGGAAGGAAAAACAGCACAAATTTACTCAGCTATTCAGACGGGTGGTAAGTTGGGAATG
CAAACTCTAGAAAAAGTTTTAGCTGATTTGTACAAAGCTGGAACTATTTCTTTTGAAGCAGCAATATCTAAAACTTCTAA
ACCAGATGAAGTCGAACGTCTAATTGGTGCAACAGCCCCAGTAGGAGCTAAATTACGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (44-272)

3D structure

• AlphaFold DB: K7W4D0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas aeruginosa PAK	53.314	94.809	0.505
pilT	Pseudomonas stutzeri DSM 10701	53.026	94.809	0.503
pilT	Legionella pneumophila strain Lp02	52.738	94.809	0.5
pilT	Acinetobacter baylyi ADP1	52.738	94.809	0.5
pilT	Legionella pneumophila strain ERS1305867	52.738	94.809	0.5
pilT	Acinetobacter baumannii strain A118	52.161	94.809	0.495
pilT	Acinetobacter nosocomialis M2	52.161	94.809	0.495
pilT	Acinetobacter baumannii D1279779	52.161	94.809	0.495
pilT	Neisseria gonorrhoeae MS11	50.147	92.623	0.464
pilT	Neisseria meningitidis 8013	49.853	92.623	0.462
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.415	93.443	0.462
pilT	Vibrio cholerae strain A1552	49.415	93.443	0.462
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.429	95.628	0.454