Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ANA_RS15715 Genome accession   NC_019427
Coordinates   3618878..3620878 (+) Length   666 a.a.
NCBI ID   WP_015081025.1    Uniprot ID   K7WYY7
Organism   Anabaena sp. 90     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3613878..3625878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANA_RS15705 (ANA_C13198) dnaK 3615150..3617015 (-) 1866 WP_015081023.1 molecular chaperone DnaK -
  ANA_RS15710 (ANA_C13199) grpE 3617731..3618405 (-) 675 WP_015081024.1 nucleotide exchange factor GrpE -
  ANA_RS15715 (ANA_C13200) pilB/pilB1 3618878..3620878 (+) 2001 WP_015081025.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ANA_RS15720 (ANA_C13201) pilT 3620943..3622043 (+) 1101 WP_015081026.1 type IV pilus twitching motility protein PilT Machinery gene
  ANA_RS27135 - 3622030..3622176 (-) 147 WP_200863829.1 hypothetical protein -
  ANA_RS15725 (ANA_C13202) - 3622208..3623431 (+) 1224 WP_015081027.1 type II secretion system F family protein -
  ANA_RS15730 (ANA_C13203) - 3623850..3624983 (-) 1134 WP_371260351.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74660.43 Da        Isoelectric Point: 5.3300

>NTDB_id=54441 ANA_RS15715 WP_015081025.1 3618878..3620878(+) (pilB/pilB1) [Anabaena sp. 90]
MTYSSPQRRSTALTTRTEFSPFVNKLVASGYVNNEQMKQAVIASRKSGKPLTEVLESITERKLSPEFVTEYKKQHLFELT
ILYGVESLDPAIYEIDPTNVGNLIQTLIPIDICRRHQLIPLSRNDNQSPPYVLVAMVDPDNLDAYDDINRILRSQGLSFQ
RRVITQKDYQDIINKFLNEQAARQKTLEQQKNYDVKGDLDNLETLDIDESSDNDVDLDNAMKGADQAPIIKLVNRILLKA
LNEGVSDIHIEPQEEYLRIRFRKDGILREAFDNMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRIFAGRKVDFRVNT
LPSRYGEKVVLRILDNTGTQLGLDKLITDPETLNIVKDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDRETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFKISSSLIGVLAQRLVRCVCSECRIAYTPTIEELARYGLSVSQEAQVTFYKANTIPPEEIASHKNLCQKCGGVGYN
GRCGVYEVMRMTEELQTLINKEAPTKRIKEVAVEEGMKTLLAYSLDLVRQGTTTLEEVERVTLTDDGLEAELKAKRKSGL
TCLTCSGELQPEWLDCPYCMTPRFTD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=54441 ANA_RS15715 WP_015081025.1 3618878..3620878(+) (pilB/pilB1) [Anabaena sp. 90]
ATGACTTACTCATCACCACAACGGCGTAGCACTGCGCTGACTACAAGAACTGAGTTTTCGCCTTTCGTTAATAAATTAGT
AGCGTCTGGCTATGTCAATAATGAACAAATGAAGCAGGCTGTAATTGCCAGCCGCAAGTCCGGTAAACCACTGACAGAAG
TATTAGAATCAATTACTGAGCGAAAATTATCACCTGAGTTTGTCACCGAATACAAAAAACAACATTTATTTGAACTCACA
ATTTTATACGGCGTTGAGTCTCTTGATCCCGCAATCTACGAAATTGATCCAACAAACGTGGGGAATTTAATTCAAACCCT
CATTCCTATTGATATCTGTCGTCGGCATCAGTTAATCCCATTGTCAAGAAATGATAACCAAAGCCCACCTTATGTGTTGG
TGGCTATGGTTGATCCAGATAATTTAGATGCTTATGATGATATTAATCGGATTTTGCGTTCCCAAGGCTTGTCTTTTCAA
CGCAGGGTAATTACCCAGAAAGATTATCAGGACATCATTAACAAATTTTTAAATGAACAAGCAGCACGCCAAAAGACATT
AGAGCAGCAAAAAAATTATGATGTCAAGGGTGATTTAGACAATCTAGAAACTCTGGACATAGATGAAAGTAGTGATAATG
ATGTTGATTTAGATAATGCTATGAAGGGTGCTGATCAGGCACCAATCATTAAACTTGTGAATCGGATTTTGTTGAAGGCT
TTAAATGAGGGTGTTTCGGATATTCACATTGAACCGCAGGAAGAATATTTACGGATTCGTTTCCGTAAGGATGGGATATT
ACGTGAGGCATTTGATAATATGCCGAAAAAAATTATCCCAGCGGTAACAGCCCGATTTAAAATTATTTCTAACCTGGACA
TTGCTGAAAGACGGTTACCTCAAGATGGCCGGATTCGCCGGATATTTGCCGGAAGAAAGGTGGATTTCCGGGTAAATACT
CTACCTAGTCGCTATGGAGAAAAGGTTGTTTTACGGATTTTAGATAACACTGGAACTCAATTAGGTTTGGATAAGTTAAT
TACTGACCCAGAAACCTTAAATATTGTTAAGGATATGGTTAGTAAGCCATTCGGGTTGATTTTAGTGACCGGACCAACTG
GTTCGGGTAAAACTACTTCGTTGTATTCGGCACTATCAGAAAAGAATGATCCGGGGATTAATATCAGTACAGTAGAAGAT
CCAATTGAGTATAGTTTACCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGTTGGATTTCTCGACGGCCTTGCG
GGCTTTCTTGCGTCAAGATCCAGATGTGTTGCTGGTGGGGGAAACACGGGATCGGGAAACTGCGAAAACGGCGATTGAGG
CAGCATTAACCGGTCACTTGGTATTAACTACTTTACACACTAATGATGCGCCAGGAGCGATCGCTCGCTTGGGAGAAATG
GGTATTGAACCTTTCAAGATTTCCAGTTCCTTAATTGGTGTTTTAGCACAACGTCTAGTTCGTTGTGTCTGTTCTGAATG
TCGTATAGCATACACTCCGACCATTGAAGAACTAGCTCGCTATGGTCTATCCGTTTCCCAAGAAGCTCAGGTAACTTTCT
ACAAAGCCAATACTATCCCACCAGAAGAAATAGCCAGCCATAAAAATCTTTGTCAAAAATGTGGTGGTGTTGGCTACAAC
GGGCGTTGTGGGGTTTATGAAGTAATGCGGATGACTGAAGAATTACAAACTCTGATCAACAAGGAAGCACCTACAAAAAG
GATTAAAGAAGTAGCTGTTGAAGAGGGGATGAAAACCTTACTAGCTTACAGTTTGGACTTAGTTCGTCAAGGAACAACTA
CCTTAGAAGAAGTAGAGCGAGTAACACTTACAGATGACGGTTTAGAGGCTGAATTAAAAGCTAAACGCAAAAGTGGTCTG
ACTTGTCTGACTTGTAGTGGGGAATTACAACCAGAATGGTTAGATTGTCCTTACTGTATGACACCTCGCTTTACAGATTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-501)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7WYY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.76

100

0.635

Multiple sequence alignment