Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   B5T_RS17165 Genome accession   NC_018691
Coordinates   3864719..3865195 (-) Length   158 a.a.
NCBI ID   WP_014995799.1    Uniprot ID   K0CGM3
Organism   Alloalcanivorax dieselolei B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3859719..3870195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5T_RS17145 (B5T_03467) - 3860169..3860699 (+) 531 WP_229682985.1 DUF1850 domain-containing protein -
  B5T_RS17150 (B5T_03468) - 3860696..3862906 (+) 2211 WP_014995796.1 TRAP transporter permease -
  B5T_RS17155 (B5T_03469) - 3863041..3864324 (+) 1284 WP_014995797.1 type II toxin-antitoxin system HipA family toxin -
  B5T_RS17160 (B5T_03470) - 3864325..3864597 (+) 273 WP_014995798.1 helix-turn-helix domain-containing protein -
  B5T_RS17165 (B5T_03471) pilE 3864719..3865195 (-) 477 WP_014995799.1 pilin Machinery gene
  B5T_RS17170 (B5T_03472) pilB 3865557..3867269 (+) 1713 WP_014995800.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5T_RS17175 (B5T_03473) pilC 3867341..3868564 (+) 1224 WP_014995801.1 type II secretion system F family protein Machinery gene
  B5T_RS17180 (B5T_03474) pilD 3868592..3869455 (+) 864 WP_014995802.1 A24 family peptidase Machinery gene
  B5T_RS17185 (B5T_03475) coaE 3869554..3870159 (+) 606 WP_014995803.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 15998.19 Da        Isoelectric Point: 4.3148

>NTDB_id=53749 B5T_RS17165 WP_014995799.1 3864719..3865195(-) (pilE) [Alloalcanivorax dieselolei B5]
MKNVQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEAILAGSACRTTVTEVYQSGSALPAADAWGCETSAALGN
NTAKVDSVATSAAGAITITINTTEIPQLAGANVLTLVPLTDATTAAGTAGDPIFGWRCGDTADGTTVTSQYLPSSCRG

Nucleotide


Download         Length: 477 bp        

>NTDB_id=53749 B5T_RS17165 WP_014995799.1 3864719..3865195(-) (pilE) [Alloalcanivorax dieselolei B5]
ATGAAAAACGTGCAAAAGGGTTTTACGCTGATTGAATTGATGATCGTGGTGGCGATCATCGGGATTCTGGCGGCGGTGGC
TTTGCCTGCTTATCAGGACTACACCGTGCGCGCAAAAGTATCCGAGGCCATCCTTGCTGGTTCCGCTTGCCGTACTACGG
TGACGGAGGTATACCAAAGTGGAAGTGCGTTGCCGGCTGCTGATGCCTGGGGATGTGAAACTAGTGCAGCATTGGGCAAT
AACACAGCAAAAGTTGATAGTGTTGCCACCAGTGCTGCCGGTGCTATCACGATCACGATTAATACCACTGAGATTCCGCA
ATTGGCCGGTGCCAACGTGCTGACCCTGGTGCCGCTGACCGACGCTACCACTGCTGCAGGTACCGCTGGTGATCCGATCT
TCGGCTGGCGTTGTGGCGATACTGCAGACGGTACCACCGTTACTAGCCAGTATCTTCCCAGTTCTTGCCGGGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-157)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0CGM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.212

100

0.481
  comP Acinetobacter baylyi ADP1

44.512

100

0.462
  pilA Ralstonia pseudosolanacearum GMI1000

37.912

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

43.791

96.835

0.424
  pilA2 Legionella pneumophila str. Paris

43.791

96.835

0.424
  pilE Neisseria gonorrhoeae strain FA1090

39.634

100

0.411
  pilA/pilA1 Eikenella corrodens VA1

39.024

100

0.405
  pilE Neisseria gonorrhoeae MS11

37.195

100

0.386
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.152

100

0.367
  pilA Pseudomonas aeruginosa PAK

35.185

100

0.361

Multiple sequence alignment