Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B5T_RS17170 Genome accession   NC_018691
Coordinates   3865557..3867269 (+) Length   570 a.a.
NCBI ID   WP_014995800.1    Uniprot ID   -
Organism   Alloalcanivorax dieselolei B5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3860557..3872269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5T_RS17150 (B5T_03468) - 3860696..3862906 (+) 2211 WP_014995796.1 TRAP transporter permease -
  B5T_RS17155 (B5T_03469) - 3863041..3864324 (+) 1284 WP_014995797.1 type II toxin-antitoxin system HipA family toxin -
  B5T_RS17160 (B5T_03470) - 3864325..3864597 (+) 273 WP_014995798.1 helix-turn-helix domain-containing protein -
  B5T_RS17165 (B5T_03471) pilE 3864719..3865195 (-) 477 WP_014995799.1 pilin Machinery gene
  B5T_RS17170 (B5T_03472) pilB 3865557..3867269 (+) 1713 WP_014995800.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B5T_RS17175 (B5T_03473) pilC 3867341..3868564 (+) 1224 WP_014995801.1 type II secretion system F family protein Machinery gene
  B5T_RS17180 (B5T_03474) pilD 3868592..3869455 (+) 864 WP_014995802.1 A24 family peptidase Machinery gene
  B5T_RS17185 (B5T_03475) coaE 3869554..3870159 (+) 606 WP_014995803.1 dephospho-CoA kinase -
  B5T_RS17190 (B5T_03476) - 3870149..3870343 (+) 195 WP_014995804.1 DNA gyrase inhibitor YacG -
  B5T_RS17195 (B5T_03477) - 3870344..3871294 (-) 951 WP_014995805.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62424.26 Da        Isoelectric Point: 5.6276

>NTDB_id=53750 B5T_RS17170 WP_014995800.1 3865557..3867269(+) (pilB) [Alloalcanivorax dieselolei B5]
MSSPQSSLSGLARRLVNEGHLSTEQVMEAAGAARREQMPLVRRLVGSGLLTAATVARASAEEFGDPVIDLDAFSPDVLVK
DLVDTKLIARHTVLPLYKRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEDDKLTRWIDRLESEAEGKAFADLDEGL
DNLDVANGEPAADSKDESNGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGVLQTMSKPPINSASKIAA
RLKVMARLDISERRVPQDGRIKLKISKNRAIDFRVNTCPTLFGEKIVLRILDPDSAKMGIDALGYEPEQKDMYMRALNQP
QGMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPRQGLDFSSALRAFLRQDPDVILVGEIR
DLETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCSHCKEPVDVPKPSLLAMGF
TEPDVESGFTVFGPKGCQKCNNGYRGRVGIYEVVRVTDGIARIIMQEGNSIEIADQCRKEGFNDLRRSGLLKVMQGVTSL
EEMDRVTSGH

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=53750 B5T_RS17170 WP_014995800.1 3865557..3867269(+) (pilB) [Alloalcanivorax dieselolei B5]
ATGAGCAGTCCTCAATCATCCCTGAGCGGCCTCGCCCGCCGCCTGGTCAACGAAGGCCATCTGTCCACCGAGCAGGTGAT
GGAAGCCGCTGGTGCTGCCCGTCGAGAGCAGATGCCTCTGGTCCGCCGTCTGGTGGGCTCGGGTCTGCTGACCGCCGCCA
CCGTGGCACGGGCCTCCGCCGAGGAGTTCGGGGATCCGGTCATAGATCTGGATGCGTTCTCCCCGGATGTCCTGGTCAAG
GATCTGGTGGATACCAAGCTGATTGCCCGCCACACCGTCCTGCCCCTGTATAAAAGGGGCAGTCGTTTGTTCGTCGCCGT
CTCCGACCCCACCAATCTGCCGGCGCTGGAGGAAATCCGCTTCAATACCGGCCTCAACGTGGAAACGGTGATCGTCGAAG
ACGACAAGCTGACCCGTTGGATCGACCGGTTGGAATCGGAGGCGGAGGGCAAGGCGTTCGCCGATCTCGACGAGGGCCTG
GACAATCTGGACGTGGCCAACGGCGAACCGGCCGCGGATTCCAAGGATGAAAGTAACGGCGCCGACGATGCCCCCATCGT
TCGCTTCGTCAACAAACTGCTGCTGGACGCCATCAAGGGCGGCGCCTCGGACCTGCACTTCGAGCCCTACGAAAAGACCT
ACCGCGTACGCTTCCGTCAGGACGGTGTGTTGCAGACCATGTCCAAACCTCCGATCAACAGCGCCAGCAAGATCGCCGCC
CGGCTCAAGGTGATGGCGCGCCTGGATATTTCCGAACGGCGGGTGCCCCAGGACGGTCGCATCAAGCTGAAGATTTCCAA
GAATCGCGCCATCGATTTCCGGGTCAACACCTGCCCCACTCTGTTTGGCGAAAAGATCGTGCTGCGGATTCTGGATCCGG
ACAGCGCCAAGATGGGCATCGATGCTCTGGGCTATGAGCCCGAGCAAAAAGACATGTACATGAGAGCCCTGAACCAGCCC
CAGGGCATGATCCTGGTCACCGGCCCCACCGGTTCTGGTAAGACCGTGTCTCTCTATACAGGTGTGAATATTCTCAACAC
GCCGGAGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTGAATCCCA
GGCAGGGGCTGGATTTCTCGTCCGCGCTACGAGCGTTCCTGCGTCAGGATCCCGACGTTATTCTGGTGGGGGAGATCCGC
GATCTGGAGACCGCGGAAATCGCCGTGAAGGCGGCCCAGACCGGCCACCTGGTGTTATCGACCCTGCACACCAACAGCGC
TCCGGAAACCCTGACCCGTCTGCGTAACATGGGGGTGCCGTCCTTCAATATCGCCACCTCGGTGAGTCTGATCATCGCCC
AGCGCCTGGCCCGCCGCCTCTGCAGCCACTGCAAGGAACCGGTGGATGTGCCCAAACCCTCGCTGCTTGCGATGGGTTTC
ACGGAACCGGACGTCGAGAGTGGCTTTACTGTGTTCGGCCCGAAAGGCTGCCAAAAATGCAATAACGGCTACAGGGGCCG
GGTCGGGATCTATGAAGTGGTGCGGGTCACGGACGGCATTGCCCGCATCATCATGCAGGAAGGGAATTCCATCGAAATCG
CCGACCAGTGCCGCAAGGAAGGCTTCAACGATCTGCGCCGCTCCGGGCTGCTCAAGGTGATGCAGGGGGTCACCAGTCTG
GAGGAAATGGACCGGGTCACCAGCGGCCACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.214

99.825

0.621
  pilB Acinetobacter baylyi ADP1

61.101

98.772

0.604
  pilB Legionella pneumophila strain ERS1305867

52.531

100

0.528
  pilF Neisseria gonorrhoeae MS11

48.936

98.947

0.484
  pilB Vibrio cholerae strain A1552

53.922

89.474

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.036

93.158

0.475
  pilB Vibrio campbellii strain DS40M4

50.76

92.281

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

41.667

90.526

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

92.456

0.37
  pilF Thermus thermophilus HB27

38.018

97.368

0.37

Multiple sequence alignment