Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6J77_RS14925 Genome accession   NZ_CP069535
Coordinates   3285887..3286999 (+) Length   370 a.a.
NCBI ID   WP_007807540.1    Uniprot ID   I4W1E4
Organism   Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3280887..3291999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J77_RS14905 (I6J77_14905) - 3282523..3283893 (-) 1371 WP_056766717.1 pyridoxal-phosphate dependent enzyme -
  I6J77_RS14910 (I6J77_14910) - 3284050..3284277 (-) 228 WP_056717360.1 YdcH family protein -
  I6J77_RS14915 (I6J77_14915) - 3284603..3285244 (-) 642 WP_204109615.1 hypothetical protein -
  I6J77_RS14920 (I6J77_14920) - 3285241..3285801 (-) 561 WP_204109616.1 DUF4398 domain-containing protein -
  I6J77_RS14925 (I6J77_14925) pilU 3285887..3286999 (+) 1113 WP_007807540.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6J77_RS14930 (I6J77_14930) maiA 3287213..3287875 (-) 663 WP_204109617.1 maleylacetoacetate isomerase -
  I6J77_RS14935 (I6J77_14935) - 3288048..3289232 (+) 1185 WP_204109618.1 phosphoglycerate kinase -
  I6J77_RS14940 (I6J77_14940) - 3289232..3289870 (+) 639 WP_204109619.1 HAD hydrolase-like protein -
  I6J77_RS14945 (I6J77_14945) tkt 3289936..3291945 (-) 2010 WP_204109620.1 transketolase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41185.38 Da        Isoelectric Point: 6.3531

>NTDB_id=535400 I6J77_RS14925 WP_007807540.1 3285887..3286999(+) (pilU) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
MDIGYFLKLMVDKGASDMFLSTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIEQLQLPKIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDHRNTNSSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHTYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRIPAAEVLLNTPLIRDMIRRGQTHEIKEAMDRSLQEGMQTFDQSL
YRLYKEGRVDLDEALNKSDSRDGLALKIRLAEGGTSDEELAGNDPYGMGF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=535400 I6J77_RS14925 WP_007807540.1 3285887..3286999(+) (pilU) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
GTGGACATCGGTTACTTCCTGAAGCTTATGGTAGACAAGGGCGCGTCGGACATGTTCCTGTCCACGGGCGCCCCGGTGAA
TATCAAGGTCGAGGGCAAACTGTATCCGCTGGGCAATACCGGCCTTCCCAGCGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCCCAGTTCGAGCGCGACCTTGAATTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATCCCCAGCATCGA
GCAGCTGCAGCTGCCGAAGATCTTCCGCGACCTGATCATGGAGCCGCGCGGCCTGATCCTGGTGGTCGGCGCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACCACCGCAACACGAATTCCTCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTGGGCCTGGACACCCATACCTATCACGA
GGCCCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGACACCATGGAGGCGG
CGATCGCCTTCTCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCGAACAACGCCGACCAGACGCTGGAACGCATC
CTCAACTTCTTCCCCGAATCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
TCTGGTGATCGGCAAGGACGGCCGGCGCATTCCCGCCGCCGAGGTACTGCTGAACACCCCGCTGATCCGCGACATGATCC
GCCGTGGCCAGACCCACGAGATCAAGGAGGCGATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTTGACCAGTCACTG
TACCGCCTGTACAAGGAAGGCCGCGTGGACCTGGACGAAGCGCTCAACAAGTCCGACTCGCGCGACGGCCTGGCCCTGAA
GATCCGCCTGGCCGAAGGCGGCACCAGCGACGAGGAACTGGCCGGCAACGACCCGTACGGGATGGGCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4W1E4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.556

97.297

0.492
  pilU Vibrio cholerae strain A1552

47.568

100

0.476
  pilU Acinetobacter baylyi ADP1

48.87

95.676

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

91.622

0.411
  pilT Acinetobacter baylyi ADP1

42.814

90.27

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.351

0.378
  pilT Acinetobacter nosocomialis M2

42.813

88.378

0.378
  pilT Acinetobacter baumannii D1279779

42.813

88.378

0.378
  pilT Acinetobacter baumannii strain A118

42.813

88.378

0.378
  pilT Pseudomonas aeruginosa PAK

41.124

91.351

0.376
  pilT Legionella pneumophila strain Lp02

40.643

92.432

0.376
  pilT Legionella pneumophila strain ERS1305867

40.643

92.432

0.376
  pilT Vibrio cholerae strain A1552

40.653

91.081

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

91.081

0.37