Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JHW41_RS04970 Genome accession   NZ_CP067396
Coordinates   1147683..1148798 (-) Length   371 a.a.
NCBI ID   WP_057948907.1    Uniprot ID   A0A0S2DM43
Organism   Lysobacter enzymogenes strain CX06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1142683..1153798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW41_RS04940 (JHW41_04910) rplM 1143554..1143982 (+) 429 WP_057948912.1 50S ribosomal protein L13 -
  JHW41_RS04945 (JHW41_04915) rpsI 1143998..1144390 (+) 393 WP_057948911.1 30S ribosomal protein S9 -
  JHW41_RS04955 (JHW41_04925) - 1144802..1145320 (-) 519 WP_250449224.1 GNAT family N-acetyltransferase -
  JHW41_RS04960 (JHW41_04930) - 1145561..1146541 (+) 981 WP_123646873.1 fumarylacetoacetate hydrolase family protein -
  JHW41_RS04965 (JHW41_04935) maiA 1146823..1147485 (+) 663 WP_057948908.1 maleylacetoacetate isomerase -
  JHW41_RS04970 (JHW41_04940) pilU 1147683..1148798 (-) 1116 WP_057948907.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JHW41_RS04975 (JHW41_04945) - 1148902..1149279 (+) 378 WP_078996049.1 DUF4398 domain-containing protein -
  JHW41_RS04980 (JHW41_04950) - 1149276..1149902 (+) 627 WP_250449225.1 hypothetical protein -
  JHW41_RS04985 (JHW41_04955) - 1150607..1150825 (+) 219 WP_057948905.1 YdcH family protein -
  JHW41_RS04990 (JHW41_04960) - 1151351..1152721 (+) 1371 WP_250449226.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41053.32 Da        Isoelectric Point: 6.1908

>NTDB_id=525861 JHW41_RS04970 WP_057948907.1 1147683..1148798(-) (pilU) [Lysobacter enzymogenes strain CX06]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKSIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGTDGRRIPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDASAAH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=525861 JHW41_RS04970 WP_057948907.1 1147683..1148798(-) (pilU) [Lysobacter enzymogenes strain CX06]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACTGGCGCGCCGGTCTA
CATCAAGGTCGAGGGGCGCCTGTACCCGCTCGGCAACACCGGCTTGCCGCCGGGAATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTCCCGCAGTTCGAGCGCGACCTCGAACTCAACATGGCGCTGGCCCTGCCCGACGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCAGCATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGAGCATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGATCACCGCAACAGCAACACCGCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCACCACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTCAAGGCGGTGGTGTCGCAGCG
CCTGGTGGTCGGCACCGACGGACGCCGCATCCCGGCCGCGGAGGTGCTGATCAACACCCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCAGGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCACCGGCGAGCACGATCCGTACGCGGACATGTACGACGCGTCGGCGGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2DM43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Acinetobacter baylyi ADP1

49.153

95.418

0.469
  pilU Vibrio cholerae strain A1552

48.305

95.418

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.723

91.375

0.418
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Legionella pneumophila strain Lp02

40.237

91.105

0.367
  pilT Legionella pneumophila strain ERS1305867

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

38.617

93.531

0.361
  pilT Neisseria meningitidis 8013

38.617

93.531

0.361