Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JHW38_RS11870 Genome accession   NZ_CP067395
Coordinates   2852596..2853711 (+) Length   371 a.a.
NCBI ID   WP_207526106.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2847596..2858711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS11850 (JHW38_11850) - 2848724..2850094 (-) 1371 WP_207526102.1 pyridoxal-phosphate dependent enzyme -
  JHW38_RS11855 (JHW38_11855) - 2850597..2850815 (-) 219 WP_207526103.1 YdcH family protein -
  JHW38_RS11860 (JHW38_11860) - 2851493..2852119 (-) 627 WP_207526104.1 hypothetical protein -
  JHW38_RS11865 (JHW38_11865) - 2852116..2852493 (-) 378 WP_207526105.1 DUF4398 domain-containing protein -
  JHW38_RS11870 (JHW38_11870) pilU 2852596..2853711 (+) 1116 WP_207526106.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JHW38_RS11875 (JHW38_11875) maiA 2853842..2854504 (-) 663 WP_207526107.1 maleylacetoacetate isomerase -
  JHW38_RS11880 (JHW38_11880) - 2854765..2855745 (-) 981 WP_207526108.1 fumarylacetoacetate hydrolase family protein -
  JHW38_RS11885 (JHW38_11885) - 2856139..2858118 (+) 1980 WP_207526109.1 peptidoglycan DD-metalloendopeptidase family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41069.32 Da        Isoelectric Point: 6.1908

>NTDB_id=525853 JHW38_RS11870 WP_207526106.1 2852596..2853711(+) (pilU) [Lysobacter enzymogenes strain CX03]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKSIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGTDGRRIPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGSGEHDPYADMYDASATH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=525853 JHW38_RS11870 WP_207526106.1 2852596..2853711(+) (pilU) [Lysobacter enzymogenes strain CX03]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTTTTGACCACCGGCGCACCGGTCTA
CATCAAGGTCGAGGGGCGCCTGTACCCGCTCGGCAACACCGGTTTGCCGCCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTTCCGCAGTTCGAGCGTGACCTCGAACTGAACATGGCGCTCGCCCTGCCCGACGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGAGCATCATCATGGCCCCGCGCGGGCTGGTGCTGATCGTAGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACCACCGCAACAGCAACACCGCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCTTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCACCACGATGGAGGCGG
CGATCGCGTTCGCCGAAACCGGCCATCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCCTGGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTCAAGGCGGTGGTGTCGCAGCG
CCTGGTGGTCGGCACCGACGGGCGCCGCATCCCGGCCGCGGAAGTGCTGATCAACACTCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCAGGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCAGCGGCGAGCACGATCCGTATGCGGACATGTACGACGCGTCGGCGACGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Acinetobacter baylyi ADP1

49.153

95.418

0.469
  pilU Vibrio cholerae strain A1552

48.333

97.035

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.723

91.375

0.418
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Legionella pneumophila strain Lp02

40.237

91.105

0.367
  pilT Legionella pneumophila strain ERS1305867

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

38.617

93.531

0.361
  pilT Neisseria meningitidis 8013

38.617

93.531

0.361