Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JH260_RS16055 Genome accession   NZ_CP066959
Coordinates   3655809..3656234 (-) Length   141 a.a.
NCBI ID   WP_031423879.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. incanae strain 4053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3642677..3659353 3655809..3656234 within 0


Gene organization within MGE regions


Location: 3642677..3659353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH260_RS16000 (JH260_15970) - 3642677..3643087 (-) 411 WP_323549302.1 CopG family ribbon-helix-helix protein -
  JH260_RS16005 (JH260_15975) sucD 3643191..3644066 (-) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH260_RS16010 (JH260_15980) sucC 3644091..3645260 (-) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  JH260_RS16015 (JH260_15985) - 3645494..3647104 (+) 1611 WP_116901781.1 PAS domain-containing sensor histidine kinase -
  JH260_RS16020 (JH260_15990) pilR 3647313..3648707 (+) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  JH260_RS16025 (JH260_15995) - 3648905..3649261 (+) 357 WP_139328384.1 hypothetical protein -
  JH260_RS16030 (JH260_16000) - 3649206..3649997 (+) 792 WP_274394332.1 zeta toxin family protein -
  JH260_RS16035 (JH260_16005) pilB 3650180..3651913 (-) 1734 WP_274394333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH260_RS16040 (JH260_16010) - 3651948..3653474 (-) 1527 WP_338000692.1 hypothetical protein -
  JH260_RS16045 (JH260_16015) - 3653444..3654073 (-) 630 WP_274394335.1 hypothetical protein -
  JH260_RS16050 (JH260_16020) - 3654084..3655673 (-) 1590 WP_218554304.1 phosphoethanolamine transferase -
  JH260_RS16055 (JH260_16025) pilA2 3655809..3656234 (-) 426 WP_031423879.1 pilin Machinery gene
  JH260_RS16060 (JH260_16030) pilC 3656587..3657846 (+) 1260 WP_274394336.1 type II secretion system F family protein Machinery gene
  JH260_RS16065 (JH260_16035) - 3657853..3658716 (+) 864 WP_040940797.1 A24 family peptidase -
  JH260_RS16070 (JH260_16040) coaE 3658730..3659353 (+) 624 WP_228424129.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14543.75 Da        Isoelectric Point: 9.0169

>NTDB_id=523619 JH260_RS16055 WP_031423879.1 3655809..3656234(-) (pilA2) [Xanthomonas campestris pv. incanae strain 4053]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVTNVTAANSGYVFPGATK
YVSNVAITNATGRVTVTSTVPNATGDILLTPTDVGGGQLKWTCSSAINTKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=523619 JH260_RS16055 WP_031423879.1 3655809..3656234(-) (pilA2) [Xanthomonas campestris pv. incanae strain 4053]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCTTATCAGGATTACACCACTCGCGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAGCTTGCGGTAA
CTGAGACTGCATCGTCGCTCGGCGGTGTTACTAATGTTACTGCTGCGAATTCGGGCTACGTATTCCCGGGTGCCACGAAG
TATGTCAGCAATGTCGCCATCACCAATGCAACGGGTCGCGTCACTGTGACCTCCACGGTGCCCAACGCTACCGGTGACAT
CCTGCTGACCCCGACTGATGTCGGTGGTGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCAACACCAAGTACCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.857

99.291

0.525
  pilA2 Legionella pneumophila str. Paris

52.857

99.291

0.525
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.45

100

0.475
  pilE Neisseria gonorrhoeae MS11

39.264

100

0.454
  pilA Ralstonia pseudosolanacearum GMI1000

39.024

100

0.454
  comP Acinetobacter baylyi ADP1

42.177

100

0.44
  pilA/pilA1 Eikenella corrodens VA1

40.667

100

0.433
  pilE Neisseria gonorrhoeae strain FA1090

37.423

100

0.433
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.754

97.872

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.397
  pilA Acinetobacter baumannii strain A118

38.194

100

0.39
  pilA Haemophilus influenzae 86-028NP

42.149

85.816

0.362