Detailed information NTG0523618

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: JH260_RS16035
• Genome accession: NZ_CP066959
• Coordinates: 3650180..3651913 (-)
• Length: 577 a.a.
• NCBI ID: WP_274394333.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. incanae strain 4053
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3642677..3659353	3650180..3651913	within	0

Gene organization within MGE regions

Location: 3642677..3659353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH260_RS16000 (JH260_15970)	-	3642677..3643087 (-)	411	WP_323549302.1	CopG family ribbon-helix-helix protein	-
JH260_RS16005 (JH260_15975)	sucD	3643191..3644066 (-)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
JH260_RS16010 (JH260_15980)	sucC	3644091..3645260 (-)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
JH260_RS16015 (JH260_15985)	-	3645494..3647104 (+)	1611	WP_116901781.1	PAS domain-containing sensor histidine kinase	-
JH260_RS16020 (JH260_15990)	pilR	3647313..3648707 (+)	1395	WP_043921909.1	sigma-54 dependent transcriptional regulator	Regulator
JH260_RS16025 (JH260_15995)	-	3648905..3649261 (+)	357	WP_139328384.1	hypothetical protein	-
JH260_RS16030 (JH260_16000)	-	3649206..3649997 (+)	792	WP_274394332.1	zeta toxin family protein	-
JH260_RS16035 (JH260_16005)	pilB	3650180..3651913 (-)	1734	WP_274394333.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH260_RS16040 (JH260_16010)	-	3651948..3653474 (-)	1527	WP_338000692.1	hypothetical protein	-
JH260_RS16045 (JH260_16015)	-	3653444..3654073 (-)	630	WP_274394335.1	hypothetical protein	-
JH260_RS16050 (JH260_16020)	-	3654084..3655673 (-)	1590	WP_218554304.1	phosphoethanolamine transferase	-
JH260_RS16055 (JH260_16025)	pilA2	3655809..3656234 (-)	426	WP_031423879.1	pilin	Machinery gene
JH260_RS16060 (JH260_16030)	pilC	3656587..3657846 (+)	1260	WP_274394336.1	type II secretion system F family protein	Machinery gene
JH260_RS16065 (JH260_16035)	-	3657853..3658716 (+)	864	WP_040940797.1	A24 family peptidase	-
JH260_RS16070 (JH260_16040)	coaE	3658730..3659353 (+)	624	WP_228424129.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62667.83 Da
• Isoelectric Point: 5.9180

>NTDB_id=523618 JH260_RS16035 WP_274394333.1 3650180..3651913(-) (pilB) [Xanthomonas campestris pv. incanae strain 4053]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTKALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGSALG
GDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELHEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=523618 JH260_RS16035 WP_274394333.1 3650180..3651913(-) (pilB) [Xanthomonas campestris pv. incanae strain 4053]
ATGAATTCAGTAGCTACCACTAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCGATGGAGCAGGCGGCCGCTGCCAAAGTGCCGCTGCCGCAGTGGTTTTCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCAGCCAACGCCGTGGAATTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCTAGT
CAGAACGCTGTCAAATTGGTAAGTGAAGAGTTGCTCCAGAAGCATCAGGTGTTGCCGCTGTTCAAGCGCGGCAACAGATT
GTTTGTGGGAGTGAGCAATCCAACCCAGACTAAGGCGTTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCAA
TCCTGGTCGATGAGGACCAGATCCGTAGGACGTTGGAGCAATGGCAGGCAGGAAACGCGTCCTTTGGCTCGGCGCTTGGT
GGCGACGACGAGGAGATGGGGGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGATGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGCTATGAGGCGGACCAACAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTTCGCTCTTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACGGGCCATATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACGCTGGTCATCGCGCAGCGTTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCCATGAAGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACTTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (50-150)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.69	98.44	0.558
pilB	Acinetobacter baylyi ADP1	55.652	99.653	0.555
pilB	Legionella pneumophila strain ERS1305867	52.289	98.44	0.515
pilB	Vibrio cholerae strain A1552	49.393	100	0.494
pilF	Neisseria gonorrhoeae MS11	49.12	98.44	0.484
pilB	Vibrio campbellii strain DS40M4	46.046	98.614	0.454
pilB	Vibrio parahaemolyticus RIMD 2210633	46.512	96.88	0.451