Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K5B69_RS25930 Genome accession   NZ_CP081346
Coordinates   5489836..5490288 (-) Length   150 a.a.
NCBI ID   WP_028681203.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5419     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5484836..5495288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5B69_RS25910 (K5B69_25910) ampD 5485531..5486097 (-) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5B69_RS25915 (K5B69_25915) - 5486248..5488503 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  K5B69_RS25920 (K5B69_25920) nadC 5488789..5489637 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5B69_RS25930 (K5B69_25930) pilA 5489836..5490288 (-) 453 WP_028681203.1 pilin Machinery gene
  K5B69_RS25935 (K5B69_25935) pilB 5490520..5492220 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5B69_RS25940 (K5B69_25940) pilC 5492224..5493444 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  K5B69_RS25945 (K5B69_25945) pilD 5493448..5494320 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K5B69_RS25950 (K5B69_25950) coaE 5494317..5494928 (+) 612 WP_179063299.1 dephospho-CoA kinase -
  K5B69_RS25955 (K5B69_25955) yacG 5494925..5495125 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15671.99 Da        Isoelectric Point: 7.7813

>NTDB_id=522289 K5B69_RS25930 WP_028681203.1 5489836..5490288(-) (pilA) [Pseudomonas aeruginosa strain SE5419]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=522289 K5B69_RS25930 WP_028681203.1 5489836..5490288(-) (pilA) [Pseudomonas aeruginosa strain SE5419]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

38.926

99.333

0.387
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA Acinetobacter nosocomialis M2

42.537

89.333

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

39.583

96

0.38