Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   K3176_RS33310 Genome accession   NZ_CP081202
Coordinates   6953977..6954450 (+) Length   157 a.a.
NCBI ID   WP_217950248.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P9W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6948977..6959450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3176_RS33285 (K3176_33285) yacG 6949145..6949345 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  K3176_RS33290 (K3176_33290) coaE 6949342..6949953 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  K3176_RS33295 (K3176_33295) pilD 6949950..6950822 (-) 873 WP_023911898.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K3176_RS33300 (K3176_33300) pilC 6950825..6952042 (-) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  K3176_RS33305 (K3176_33305) pilB 6952046..6953746 (-) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3176_RS33310 (K3176_33310) pilE 6953977..6954450 (+) 474 WP_217950248.1 pilin Machinery gene
  K3176_RS33320 (K3176_33320) nadC 6954603..6955451 (-) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K3176_RS33325 (K3176_33325) - 6955737..6957992 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  K3176_RS33330 (K3176_33330) ampD 6958143..6958709 (+) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 15991.31 Da        Isoelectric Point: 8.0955

>NTDB_id=521933 K3176_RS33310 WP_217950248.1 6953977..6954450(+) (pilE) [Pseudomonas aeruginosa strain P9W]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLGGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=521933 K3176_RS33310 WP_217950248.1 6953977..6954450(+) (pilE) [Pseudomonas aeruginosa strain P9W]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGGGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459
  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

42.593

100

0.439
  pilA2 Legionella pneumophila str. Paris

46.259

93.631

0.433
  pilA2 Legionella pneumophila strain ERS1305867

44.898

93.631

0.42
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

96.815

0.363