Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K3176_RS33300 Genome accession   NZ_CP081202
Coordinates   6950825..6952042 (-) Length   405 a.a.
NCBI ID   WP_010792214.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P9W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6945825..6957042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3176_RS33250 (K3176_33250) - 6945895..6946065 (-) 171 WP_003094672.1 DUF3094 family protein -
  K3176_RS33255 (K3176_33255) - 6946091..6946540 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  K3176_RS33260 (K3176_33260) - 6946537..6947082 (-) 546 WP_023911893.1 DUF1780 domain-containing protein -
  K3176_RS33265 (K3176_33265) - 6947215..6947640 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  K3176_RS33270 (K3176_33270) - 6947637..6948107 (+) 471 WP_023911895.1 hypothetical protein -
  K3176_RS33275 (K3176_33275) - 6948104..6948793 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  K3176_RS33280 (K3176_33280) - 6948899..6949108 (+) 210 WP_003094660.1 hypothetical protein -
  K3176_RS33285 (K3176_33285) yacG 6949145..6949345 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  K3176_RS33290 (K3176_33290) coaE 6949342..6949953 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  K3176_RS33295 (K3176_33295) pilD 6949950..6950822 (-) 873 WP_023911898.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K3176_RS33300 (K3176_33300) pilC 6950825..6952042 (-) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  K3176_RS33305 (K3176_33305) pilB 6952046..6953746 (-) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3176_RS33310 (K3176_33310) pilE 6953977..6954450 (+) 474 WP_217950248.1 pilin Machinery gene
  K3176_RS33320 (K3176_33320) nadC 6954603..6955451 (-) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44242.19 Da        Isoelectric Point: 9.8988

>NTDB_id=521931 K3176_RS33300 WP_010792214.1 6950825..6952042(-) (pilC) [Pseudomonas aeruginosa strain P9W]
MADKALKTCVFVWEGTDKKGAKVKGELAGQNTMLVKAQLRKQGINPLKVRKKGITLLGKGKRVKPMDIALFTRQMATMMG
AGVPLLQSFDIISEGFDNPNMRKLVDEIKQEVSAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVLVAIIVSAILLIKVVPQFQSVFSSFGAELPAFTMMVINLSNLLQEWWLVVLIGLFSASFAIK
ESHKRSVNFRNTVDRYMLKIPIIGGILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVSKIKQDVSTGM
QLNFSMRTTNVFPNMAIQMTAIGEESGSLDDMLGKVAAFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=521931 K3176_RS33300 WP_010792214.1 6950825..6952042(-) (pilC) [Pseudomonas aeruginosa strain P9W]
ATGGCGGATAAAGCGTTAAAAACCTGTGTTTTCGTCTGGGAAGGCACGGACAAGAAAGGGGCAAAGGTCAAGGGGGAGTT
GGCTGGCCAGAACACTATGCTGGTGAAGGCGCAACTGCGCAAACAGGGCATAAATCCGCTCAAGGTCAGAAAAAAGGGCA
TTACCCTTCTCGGCAAGGGAAAGCGCGTAAAGCCGATGGACATCGCACTATTTACTCGTCAGATGGCAACCATGATGGGT
GCTGGGGTTCCTCTCTTGCAATCGTTCGACATAATTTCCGAAGGTTTCGACAATCCCAACATGCGTAAGCTGGTCGACGA
GATCAAACAAGAGGTCTCGGCGGGTAACAGCCTGGCAAACTCCTTGCGAAAGAAACCTCTTTATTTTGACGATCTCTATT
GCAACCTGGTGGATGCAGGTGAACAATCTGGCGCCTTGGAGACTTTGCTTGACCGTGTTGCCACCTATAAGGAAAAAACC
GAATCGCTAAAAGCCAAGATAAAAAAGGCTATGACATACCCCATTGCCGTCGTCTTGGTCGCGATCATTGTTTCAGCCAT
TCTCCTGATCAAGGTCGTACCCCAGTTCCAATCCGTTTTCTCCAGTTTCGGAGCGGAACTACCAGCCTTTACAATGATGG
TGATAAACCTGTCAAATCTTTTACAGGAATGGTGGCTAGTCGTCCTCATCGGATTATTCAGCGCCAGCTTCGCCATCAAG
GAATCGCATAAGCGTTCAGTAAATTTCCGTAACACGGTAGACCGCTATATGTTGAAAATACCGATAATCGGAGGAATACT
TTACAAATCAGCCGTGGCACGTTACGCCCGTACACTTTCAACAACTTTCGCCGCAGGTGTTCCATTGGTGGAGGCACTAG
ACTCTGTCTCTGGAGCGACAGGAAACGTGGTATTCCGCAACGCGGTAAGCAAGATCAAACAGGATGTATCCACAGGTATG
CAATTGAACTTCTCCATGAGAACGACCAATGTATTCCCCAACATGGCAATCCAGATGACAGCCATCGGTGAGGAATCCGG
TTCTCTGGACGACATGTTGGGCAAGGTGGCTGCGTTCTATGAGGAGGAAGTAGACAATGCCGTCGACAATCTGACAACTC
TCATGGAACCTATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Acinetobacter baylyi ADP1

59.045

98.272

0.58
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria meningitidis 44/76-A

44.03

99.259

0.437
  pilG Neisseria gonorrhoeae MS11

43.781

99.259

0.435
  pilC Vibrio cholerae strain A1552

43.21

100

0.432
  pilC Vibrio campbellii strain DS40M4

43.182

97.778

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37