Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I9190_RS16225 Genome accession   NZ_CP066121
Coordinates   3503120..3504238 (+) Length   372 a.a.
NCBI ID   WP_004828052.1    Uniprot ID   -
Organism   Acinetobacter bereziniae strain GD0320     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3498120..3509238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9190_RS16205 (I9190_16205) - 3498727..3499512 (-) 786 WP_058971182.1 hypothetical protein -
  I9190_RS16210 (I9190_16210) - 3499690..3500523 (-) 834 WP_151780624.1 undecaprenyl-diphosphate phosphatase -
  I9190_RS16215 (I9190_16215) - 3500916..3501608 (-) 693 WP_151780623.1 YggS family pyridoxal phosphate-dependent enzyme -
  I9190_RS16220 (I9190_16220) pilT 3502055..3503092 (+) 1038 WP_005030845.1 type IV pilus twitching motility protein PilT Machinery gene
  I9190_RS16225 (I9190_16225) pilU 3503120..3504238 (+) 1119 WP_004828052.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I9190_RS16230 (I9190_16230) fur 3504314..3504751 (-) 438 WP_009585810.1 ferric iron uptake transcriptional regulator -
  I9190_RS16235 (I9190_16235) - 3504864..3505262 (+) 399 WP_058971173.1 outer membrane protein assembly factor BamE -
  I9190_RS16240 (I9190_16240) - 3505344..3505667 (-) 324 WP_151780622.1 RnfH family protein -
  I9190_RS16245 (I9190_16245) - 3505685..3506767 (-) 1083 WP_171496479.1 hypothetical protein -
  I9190_RS16250 (I9190_16250) - 3506968..3507423 (-) 456 WP_004828043.1 bacteriohemerythrin -
  I9190_RS16255 (I9190_16255) - 3507708..3508556 (-) 849 WP_151780629.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41853.20 Da        Isoelectric Point: 6.4218

>NTDB_id=517521 I9190_RS16225 WP_004828052.1 3503120..3504238(+) (pilU) [Acinetobacter bereziniae strain GD0320]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFADTRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=517521 I9190_RS16225 WP_004828052.1 3503120..3504238(+) (pilU) [Acinetobacter bereziniae strain GD0320]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAGAAAGCATCCGACTTGTTTATTACCGCAGATGTAGAACCATC
AATGAAAATTAATGGTCAAATCGTCCCTGTGGTAAAAACCAAGTTAAACGGCGAAATCATCATGCAATTGCTCAATTCAA
TCATGAGTGAAAAGCAACGCAAAGAATTTGCAGATACGCGCGAATGTAACTTTGCCATTACTAACAGAGATAAAACTGCC
CGTTTCCGTGTCAGCGCTTTTCAACAACGTGATATGCCAGGTATGGTTTTGCGTCGTATCGAAACTAAAATTCCAACCAT
GGAAGAGTTAAAACTTCCACCTATACTCAAAGATCTTGCCATGACAAAACGTGGCATCATTATCTTTGTTGGTGCGACAG
GTACCGGTAAATCCACATCTCTTGCATCGATTATTGGCTATCGCAATCAGAACTCAAAAGGTCATATCATCACCATTGAA
GATCCGATTGAATTTGTTCATGAACATGCTGGCTGTATCATCACCCAACGTGAAGTTGGTATTGATACTGACTCTTTTGA
AATTGCCTTGAAGAACACCTTGCGTCAAGCACCTGATGTTATTCTGATTGGTGAGATTCGTTCCCGTGAAACAATGGATT
ATGCGATTGCCTTTGCTGAAACTGGACATCTGGTCTTTGCGACACTACATGCCAACAATGCCAACCAAGCCATTGACCGC
ATTATTCACTTCTTTGAATCTGACCGTCATAGCCAATTGTTTATGGACTTATCTCTTAACCTTAAAGCTATGGTCGCACA
GCAATTAATTCCAACACCAGATGGTAACTCGCGTCGTGCAGCGATTGAGATTCTGATCAACTCACCACTCGTTTCAGATC
TCATCCGTAAAGGTGATGTACATGAAATCAAAGATTTGATGAAACGTTCACGCGAATTGGGTATGCAAACTTTTGACCAA
GCCTTGTTTGACCTTTATAAAGCTGGTCAGATTACCTACAAAGATGCGTTGAAACATGCAGACTCACCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGCGCGCAGTTATTGAATGCCAACAGCAAATTGACTTTTGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

54.865

99.462

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

93.011

0.401
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.942

92.204

0.368
  pilT Acinetobacter baumannii strain A118

39.942

92.204

0.368
  pilT Acinetobacter nosocomialis M2

39.942

92.204

0.368
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36