Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   I6I17_RS01820 Genome accession   NZ_CP065999
Coordinates   352465..352899 (+) Length   144 a.a.
NCBI ID   WP_115324767.1    Uniprot ID   -
Organism   Kingella denitrificans strain FDAARGOS_1060     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347465..357899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I17_RS01795 (I6I17_01795) - 348539..349075 (+) 537 WP_003783800.1 ATP/GTP-binding protein -
  I6I17_RS01800 (I6I17_01800) - 349077..349736 (+) 660 WP_003783801.1 hypothetical protein -
  I6I17_RS01805 (I6I17_01805) - 349756..350121 (+) 366 WP_003783802.1 roadblock/LC7 domain-containing protein -
  I6I17_RS01815 (I6I17_01815) recJ 350359..352068 (-) 1710 WP_003783803.1 single-stranded-DNA-specific exonuclease RecJ -
  I6I17_RS01820 (I6I17_01820) pilA/pilA1 352465..352899 (+) 435 WP_115324767.1 pilin Machinery gene
  I6I17_RS01825 (I6I17_01825) pilA/pilA1 353029..353499 (+) 471 WP_003783807.1 pilin Machinery gene
  I6I17_RS01830 (I6I17_01830) - 353722..355482 (+) 1761 WP_147285381.1 hypothetical protein -
  I6I17_RS01835 (I6I17_01835) - 355485..356372 (+) 888 WP_003783810.1 decaprenyl-phosphate phosphoribosyltransferase -
  I6I17_RS01840 (I6I17_01840) - 356369..357331 (+) 963 WP_003783812.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14717.75 Da        Isoelectric Point: 9.0590

>NTDB_id=516240 I6I17_RS01820 WP_115324767.1 352465..352899(+) (pilA/pilA1) [Kingella denitrificans strain FDAARGOS_1060]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQATEGFKATSGLQTDIGVYAADKGNLTGVDSDSALTSQASAL
SGKYFSSGAATIGANGVITVSFNNGANSGQTMTLTPSLNTANGQITKWTCAGSIAAKRLPSSCR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=516240 I6I17_RS01820 WP_115324767.1 352465..352899(+) (pilA/pilA1) [Kingella denitrificans strain FDAARGOS_1060]
ATGAAAACAATGCAAAAAGGTTTCACCCTGATCGAGTTGATGATCGTTATCGCAATTATCGGTATTTTGGCTGCCATCGC
ATTGCCTGCATACCAAGACTACACTGCACGTGCGCAAGCTACTGAAGGCTTCAAAGCAACTTCAGGCCTGCAAACTGACA
TCGGTGTTTACGCTGCCGACAAAGGTAATTTGACAGGTGTTGATTCCGACTCAGCCCTGACTTCTCAAGCTTCTGCTTTG
AGCGGTAAATACTTCTCTAGCGGTGCTGCTACTATCGGCGCTAACGGTGTGATTACCGTATCATTCAACAACGGTGCGAA
CTCTGGTCAAACAATGACTCTGACTCCATCTTTGAACACTGCCAACGGTCAAATCACCAAATGGACTTGTGCGGGTTCTA
TCGCTGCTAAACGTCTGCCTTCTAGCTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

57.895

100

0.611
  pilA2 Legionella pneumophila str. Paris

49.342

100

0.521
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.493
  pilA2 Legionella pneumophila strain ERS1305867

46.809

97.917

0.458
  pilE Neisseria gonorrhoeae strain FA1090

40.491

100

0.458
  comP Acinetobacter baylyi ADP1

43.836

100

0.444
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.568

100

0.438
  pilE Neisseria gonorrhoeae MS11

38.182

100

0.438
  pilA Ralstonia pseudosolanacearum GMI1000

37.126

100

0.431
  pilA Acinetobacter nosocomialis M2

40.278

100

0.403
  pilA Glaesserella parasuis strain SC1401

36.806

100

0.368
  pilA/pilAII Pseudomonas stutzeri DSM 10701

34.416

100

0.368
  pilA Acinetobacter baumannii strain A118

37.857

97.222

0.368