Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   I6I17_RS01825 Genome accession   NZ_CP065999
Coordinates   353029..353499 (+) Length   156 a.a.
NCBI ID   WP_003783807.1    Uniprot ID   F0F165
Organism   Kingella denitrificans strain FDAARGOS_1060     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348029..358499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I17_RS01795 (I6I17_01795) - 348539..349075 (+) 537 WP_003783800.1 ATP/GTP-binding protein -
  I6I17_RS01800 (I6I17_01800) - 349077..349736 (+) 660 WP_003783801.1 hypothetical protein -
  I6I17_RS01805 (I6I17_01805) - 349756..350121 (+) 366 WP_003783802.1 roadblock/LC7 domain-containing protein -
  I6I17_RS01815 (I6I17_01815) recJ 350359..352068 (-) 1710 WP_003783803.1 single-stranded-DNA-specific exonuclease RecJ -
  I6I17_RS01820 (I6I17_01820) pilA/pilA1 352465..352899 (+) 435 WP_115324767.1 pilin Machinery gene
  I6I17_RS01825 (I6I17_01825) pilA/pilA1 353029..353499 (+) 471 WP_003783807.1 pilin Machinery gene
  I6I17_RS01830 (I6I17_01830) - 353722..355482 (+) 1761 WP_147285381.1 hypothetical protein -
  I6I17_RS01835 (I6I17_01835) - 355485..356372 (+) 888 WP_003783810.1 decaprenyl-phosphate phosphoribosyltransferase -
  I6I17_RS01840 (I6I17_01840) - 356369..357331 (+) 963 WP_003783812.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16794.44 Da        Isoelectric Point: 7.8072

>NTDB_id=516241 I6I17_RS01825 WP_003783807.1 353029..353499(+) (pilA/pilA1) [Kingella denitrificans strain FDAARGOS_1060]
MNNAYFLMKKGFTLIELMIVIAIIGILAAIALPAYQEYVGRAQAMEAFTLSDGLRNEIAIWAAEYHSFPDAAAIANNGYI
GSQAVVLKGKYVSSGGVSVGSNGRILVTFANGNLSGQTMVLTPTLNMVNNEQIIKWKCGSDPANTVQERYLPVSCR

Nucleotide


Download         Length: 471 bp        

>NTDB_id=516241 I6I17_RS01825 WP_003783807.1 353029..353499(+) (pilA/pilA1) [Kingella denitrificans strain FDAARGOS_1060]
ATGAATAACGCATACTTTTTGATGAAAAAAGGTTTTACTTTAATTGAGTTGATGATTGTAATTGCAATTATCGGTATTTT
GGCGGCGATTGCCTTACCTGCATATCAGGAATATGTGGGGCGAGCCCAAGCGATGGAGGCATTTACATTAAGCGATGGAT
TGCGTAATGAAATTGCTATATGGGCGGCAGAATATCATAGTTTTCCTGATGCAGCAGCCATTGCAAACAATGGTTATATT
GGATCTCAAGCTGTTGTCCTAAAAGGAAAATACGTTTCGTCAGGTGGCGTGAGCGTCGGTAGTAATGGGCGGATTTTGGT
CACTTTTGCAAATGGTAACTTATCTGGACAAACCATGGTTTTAACACCAACATTGAATATGGTGAATAATGAGCAGATTA
TCAAATGGAAATGTGGTTCGGATCCAGCAAACACCGTTCAGGAACGTTATTTGCCTGTGTCATGTCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-156)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0F165

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

50

96.154

0.481
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.024

100

0.41
  pilA2 Legionella pneumophila str. Paris

40.667

96.154

0.391
  pilA2 Legionella pneumophila strain ERS1305867

40

96.154

0.385