NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	JBL37_RS19020	Genome accession	NZ_CP065948
Coordinates	4031795..4032247 (-)	Length	150 a.a.
NCBI ID	WP_023518088.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain PAM68
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4026795..4037247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JBL37_RS18995 (JBL37_19000)	-	4026932..4027645 (+)	714	WP_012613767.1	helix-turn-helix transcriptional regulator	-
JBL37_RS19000 (JBL37_19005)	-	4027815..4028057 (-)	243	Protein_3766	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
JBL37_RS19005 (JBL37_19010)	-	4028208..4030463 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
JBL37_RS19010 (JBL37_19015)	nadC	4030749..4031597 (+)	849	WP_003116246.1	carboxylating nicotinate-nucleotide diphosphorylase	-
JBL37_RS19020 (JBL37_19025)	pilA	4031795..4032247 (-)	453	WP_023518088.1	pilin	Machinery gene
JBL37_RS19025 (JBL37_19030)	pilB	4032479..4034179 (+)	1701	WP_021264132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JBL37_RS19030 (JBL37_19035)	pilC	4034183..4035403 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
JBL37_RS19035 (JBL37_19040)	pilD	4035407..4036279 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
JBL37_RS19040 (JBL37_19045)	coaE	4036276..4036887 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
JBL37_RS19045 (JBL37_19050)	yacG	4036884..4037084 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15686.02 Da Isoelectric Point: 7.7814

                                >NTDB_id=515645 JBL37_RS19020 WP_023518088.1 4031795..4032247(-) (pilA) [Pseudomonas aeruginosa strain PAM68]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=515645 JBL37_RS19020 WP_023518088.1 4031795..4032247(-) (pilA) [Pseudomonas aeruginosa strain PAM68]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	47.651	99.333	0.473
pilA	Vibrio cholerae strain A1552	47.651	99.333	0.473
pilA	Vibrio cholerae C6706	47.651	99.333	0.473
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.333	100	0.453
pilA	Acinetobacter baumannii strain A118	44.595	98.667	0.44
pilA	Vibrio parahaemolyticus RIMD 2210633	43.243	98.667	0.427
comP	Acinetobacter baylyi ADP1	42	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.958	95.333	0.4
pilA2	Legionella pneumophila strain ERS1305867	39.597	99.333	0.393
pilA2	Legionella pneumophila str. Paris	38.926	99.333	0.387
pilA/pilA1	Eikenella corrodens VA1	38.514	98.667	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	38.889	96	0.373
pilA	Acinetobacter nosocomialis M2	41.353	88.667	0.367

Detailed information NTG0515645