Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IPQ37_RS01980 Genome accession   NZ_CP065041
Coordinates   399699..400085 (-) Length   128 a.a.
NCBI ID   WP_237042617.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-16-0963-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394699..405085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPQ37_RS01960 (IPQ37_01960) coaE 395050..395652 (-) 603 WP_109374725.1 dephospho-CoA kinase -
  IPQ37_RS01965 (IPQ37_01965) pilD 395654..396532 (-) 879 WP_005297598.1 prepilin peptidase Machinery gene
  IPQ37_RS01970 (IPQ37_01970) pilC 396715..397941 (-) 1227 WP_005297600.1 type II secretion system F family protein Machinery gene
  IPQ37_RS01975 (IPQ37_01975) pilB 398007..399698 (-) 1692 WP_237042616.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IPQ37_RS01980 (IPQ37_01980) pilA 399699..400085 (-) 387 WP_237042617.1 pilin Machinery gene
  IPQ37_RS01985 (IPQ37_01985) ampD 400471..401049 (+) 579 WP_069531889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IPQ37_RS01990 (IPQ37_01990) pdhR 401409..402182 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IPQ37_RS01995 (IPQ37_01995) aceE 402263..404923 (+) 2661 WP_106261934.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 128 a.a.        Molecular weight: 12858.69 Da        Isoelectric Point: 7.1311

>NTDB_id=506134 IPQ37_RS01980 WP_237042617.1 399699..400085(-) (pilA) [Photobacterium damselae subsp. damselae strain AS-16-0963-3]
MKGQKGFTLIELMIVVAVIGVLSAIAVPKYQEYVKKGALGAALASVTAHKTNVEDSIATLGTFPSVSAAFGIGSISSPGS
SDAVGTITATIDEGAANNATVTISRDANGVWTCTHSLSTTIKISGCQG

Nucleotide


Download         Length: 387 bp        

>NTDB_id=506134 IPQ37_RS01980 WP_237042617.1 399699..400085(-) (pilA) [Photobacterium damselae subsp. damselae strain AS-16-0963-3]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGGTTATTGGTGTGTTATCAGCAATTGCCGT
ACCAAAATATCAAGAATATGTAAAAAAAGGAGCATTAGGAGCTGCATTAGCTTCAGTAACTGCTCACAAAACTAATGTAG
AAGACTCAATTGCAACTTTAGGTACATTTCCTTCTGTTAGTGCCGCATTTGGGATAGGTAGTATTTCATCTCCTGGCTCA
TCAGACGCCGTTGGTACAATTACAGCAACAATAGATGAAGGTGCAGCCAATAATGCAACAGTAACTATCTCAAGAGATGC
AAATGGAGTTTGGACATGTACACACTCTCTTAGCACAACAATAAAGATCAGTGGTTGTCAAGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-122)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.714

100

0.555
  pilA Vibrio cholerae strain A1552

50.714

100

0.555
  pilA Vibrio cholerae C6706

50.714

100

0.555
  pilA Pseudomonas aeruginosa PAK

40.541

100

0.469
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.045

100

0.43
  pilA Vibrio parahaemolyticus RIMD 2210633

38.686

100

0.414
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.098

100

0.406
  comP Acinetobacter baylyi ADP1

34.694

100

0.398
  pilA Acinetobacter baumannii strain A118

36.496

100

0.391
  pilA/pilA1 Eikenella corrodens VA1

36.364

100

0.375
  pilA2 Legionella pneumophila str. Paris

40.336

92.969

0.375
  pilA2 Legionella pneumophila strain ERS1305867

39.496

92.969

0.367