Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J9247_RS24670 Genome accession   NZ_CP072783
Coordinates   5297040..5297495 (-) Length   151 a.a.
NCBI ID   WP_057389195.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LICME WGH-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5292040..5302495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9247_RS24650 (J9247_24650) ampD 5292746..5293312 (-) 567 WP_033970467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J9247_RS24655 (J9247_24655) - 5293462..5295717 (-) 2256 WP_210224833.1 DUF1631 domain-containing protein -
  J9247_RS24660 (J9247_24660) nadC 5296003..5296851 (+) 849 WP_033970464.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J9247_RS24670 (J9247_24670) pilA 5297040..5297495 (-) 456 WP_057389195.1 pilin Machinery gene
  J9247_RS24675 (J9247_24675) pilB 5297727..5299427 (+) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J9247_RS24680 (J9247_24680) pilC 5299431..5300651 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  J9247_RS24685 (J9247_24685) pilD 5300655..5301527 (+) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  J9247_RS24690 (J9247_24690) coaE 5301524..5302135 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  J9247_RS24695 (J9247_24695) yacG 5302132..5302332 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15876.27 Da        Isoelectric Point: 8.4827

>NTDB_id=484928 J9247_RS24670 WP_057389195.1 5297040..5297495(-) (pilA) [Pseudomonas aeruginosa strain LICME WGH-6]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASINPLKTTVEEALSRGWSVRSGTGAEDAAKKEVPLG
VDGKANNLGTIALKPDPANGAADITLTFTMDAAGPKNKDKVITLTRTATDGLWKCTSDQEKQFIPKGCSDK

Nucleotide


Download         Length: 456 bp        

>NTDB_id=484928 J9247_RS24670 WP_057389195.1 5297040..5297495(-) (pilA) [Pseudomonas aeruginosa strain LICME WGH-6]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAATACCAGAACTATGTTGCGCGCTCGGAAGGCGCTTCGGCGCTGGCGTCCATCAACCCGTTGAAGACAACTGTAG
AAGAGGCTCTTTCTCGTGGTTGGAGCGTTAGGAGTGGTACTGGTGCAGAAGACGCTGCTAAAAAAGAAGTTCCGCTGGGT
GTAGATGGAAAGGCCAATAATCTCGGCACCATTGCTCTGAAGCCTGATCCTGCAAATGGTGCTGCTGATATTACTCTGAC
CTTCACCATGGATGCTGCTGGTCCGAAGAACAAAGACAAAGTCATTACTCTGACTCGTACTGCTACTGATGGCCTTTGGA
AGTGCACCAGTGATCAGGAAAAGCAGTTCATTCCGAAAGGCTGCTCTGACAAGTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

88.591

98.675

0.874
  pilA Acinetobacter baumannii strain A118

44.371

100

0.444
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44

99.338

0.437
  pilA Vibrio cholerae strain A1552

44

99.338

0.437
  pilA Vibrio cholerae C6706

44

99.338

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.421

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.205

96.689

0.437
  pilA Vibrio parahaemolyticus RIMD 2210633

47.761

88.742

0.424
  comP Acinetobacter baylyi ADP1

41.333

99.338

0.411
  pilA2 Legionella pneumophila str. Paris

40.136

97.351

0.391
  pilA2 Legionella pneumophila strain ERS1305867

39.456

97.351

0.384
  pilA/pilA1 Eikenella corrodens VA1

36.25

100

0.384
  pilA Acinetobacter nosocomialis M2

46.61

78.146

0.364