Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ID616_RS28065 Genome accession   NZ_CP061723
Coordinates   6177465..6178460 (-) Length   331 a.a.
NCBI ID   WP_060516924.1    Uniprot ID   -
Organism   Pseudomonas putida strain ZXPA-20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6172465..6183460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ID616_RS28045 (ID616_28045) argS 6173699..6175435 (+) 1737 WP_016489632.1 arginine--tRNA ligase -
  ID616_RS28050 (ID616_28050) - 6175437..6176135 (+) 699 WP_016489633.1 SPOR domain-containing protein -
  ID616_RS28055 (ID616_28055) - 6176202..6176618 (-) 417 WP_016489634.1 NINE protein -
  ID616_RS28060 (ID616_28060) - 6176785..6177411 (+) 627 WP_016489635.1 C40 family peptidase -
  ID616_RS28065 (ID616_28065) pilT 6177465..6178460 (-) 996 WP_060516924.1 type IV pilus twitching motility protein PilT Machinery gene
  ID616_RS28070 (ID616_28070) - 6178526..6179212 (+) 687 WP_016489637.1 YggS family pyridoxal phosphate-dependent enzyme -
  ID616_RS28075 (ID616_28075) proC 6179251..6180069 (+) 819 WP_016489638.1 pyrroline-5-carboxylate reductase -
  ID616_RS28080 (ID616_28080) - 6180080..6180670 (+) 591 WP_016489639.1 YggT family protein -
  ID616_RS28085 (ID616_28085) metX 6180759..6181898 (+) 1140 WP_016489640.1 homoserine O-succinyltransferase MetX -
  ID616_RS28090 (ID616_28090) metW 6181906..6182526 (+) 621 WP_191087072.1 methionine biosynthesis protein MetW -
  ID616_RS28095 (ID616_28095) - 6182549..6182983 (+) 435 WP_016489642.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35651.18 Da        Isoelectric Point: 6.7623

>NTDB_id=484407 ID616_RS28065 WP_060516924.1 6177465..6178460(-) (pilT) [Pseudomonas putida strain ZXPA-20]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLEGDLQRLDMPPLVPADLADGMAALLAERQRRQWAEGDELDLALQLPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVGELDLGDVYQAVAQCTDGLVLVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLINQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLESVRLALRAAETGHLVLATVHTRSAASSIDRL
VEVFAAEEKPLVRAMLAESLRLVVVQTLVRRVDGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQGGASQGMRTMEGAM
RGLKESGLIRG

Nucleotide


Download         Length: 996 bp        

>NTDB_id=484407 ID616_RS28065 WP_060516924.1 6177465..6178460(-) (pilT) [Pseudomonas putida strain ZXPA-20]
ATGGATGTGACCGACCTGTTGGCCCGTGCTGTGGATGCAGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATCCCGAT
GCTGCGCCTTGAGGGCGACTTGCAGCGCCTGGACATGCCGCCGTTGGTGCCGGCAGACCTGGCCGACGGCATGGCGGCCT
TGCTGGCTGAACGCCAACGGCGGCAATGGGCAGAGGGCGATGAGCTGGACCTGGCGCTGCAGCTGCCCGCCCTGGGCCGT
TTTCGGCTGAACCTGTTTCGCCAGTTGAACGGCCCTGCGGTTACCTTCCGGTTGATCCCGGGGCGGATCGCCACGGTCGG
TGAACTCGACCTAGGGGATGTGTATCAAGCTGTTGCGCAGTGCACTGATGGCCTGGTCCTGGTAGGTGGGCCTACGGGCA
GTGGCAAGTCCAGCACCCTGGCGACCTTGCTCGACCAACTGAACCGCGACCGGGCGCTGCATATCATCACCCTCGAAGAC
CCCGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGATCAATCAGCGTGAGATTGGCCGCCATAGCGGCGGGTTCGCCCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCTGATGTGATCATGATCGGTGAGCTACGGGACCTGGAATCCGTTCGCCTGG
CCTTGCGCGCCGCCGAGACCGGGCACCTGGTGCTGGCGACCGTGCATACGCGCTCGGCGGCCAGCAGCATTGACCGATTG
GTGGAGGTATTTGCTGCCGAGGAAAAACCGTTGGTGCGGGCAATGCTGGCCGAATCGTTACGCCTCGTGGTGGTGCAGAC
GCTGGTCCGGCGTGTGGACGGTGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGCAACCTGGTTC
GGGAGGGGCGAATGGCGCAGTTATGTTCGGTGATGCAGGGCGGGGCGTCGCAGGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGCTTAAGGAGAGCGGACTGATCAGAGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.396

0.508
  pilT Acinetobacter nosocomialis M2

50.152

99.396

0.498
  pilT Acinetobacter baumannii D1279779

50.152

99.396

0.498
  pilT Acinetobacter baumannii strain A118

50.152

99.396

0.498
  pilT Pseudomonas stutzeri DSM 10701

49.848

99.396

0.495
  pilT Acinetobacter baylyi ADP1

49.544

99.396

0.492
  pilT Vibrio cholerae strain A1552

48.328

99.396

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.328

99.396

0.48
  pilT Neisseria meningitidis 8013

47.576

99.698

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.576

99.698

0.474
  pilT Neisseria gonorrhoeae MS11

47.273

99.698

0.471
  pilT Legionella pneumophila strain Lp02

47.112

99.396

0.468
  pilT Legionella pneumophila strain ERS1305867

47.112

99.396

0.468
  pilU Pseudomonas stutzeri DSM 10701

37.273

99.698

0.372