Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   XHV734_RS05355 Genome accession   NZ_CP060399
Coordinates   1298977..1299405 (+) Length   142 a.a.
NCBI ID   WP_180313578.1    Uniprot ID   A0A6V7F519
Organism   Xanthomonas hortorum pv. vitians strain LM16734     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1293977..1304405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XHV734_RS05325 (XHV734_1244) - 1294340..1294642 (-) 303 WP_074058085.1 type II toxin-antitoxin system RelE/ParE family toxin -
  XHV734_RS05330 (XHV734_1245) - 1294639..1294824 (-) 186 WP_006448358.1 hypothetical protein -
  XHV734_RS05335 (XHV734_1246) - 1294922..1295323 (-) 402 WP_074058084.1 SymE family type I addiction module toxin -
  XHV734_RS05340 (XHV734_1248) coaE 1295857..1296480 (-) 624 WP_154844506.1 dephospho-CoA kinase -
  XHV734_RS05345 (XHV734_1249) - 1296494..1297357 (-) 864 WP_002812278.1 A24 family peptidase -
  XHV734_RS05350 (XHV734_1250) pilC 1297364..1298623 (-) 1260 WP_180313588.1 type II secretion system F family protein Machinery gene
  XHV734_RS05355 (XHV734_1251) pilA2 1298977..1299405 (+) 429 WP_180313578.1 pilin Machinery gene
  XHV734_RS05360 (XHV734_1252) pilB 1299658..1301394 (+) 1737 WP_187433766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XHV734_RS05365 (XHV734_1253) pilR 1301736..1303190 (-) 1455 WP_174555994.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14495.72 Da        Isoelectric Point: 9.0168

>NTDB_id=475721 XHV734_RS05355 WP_180313578.1 1298977..1299405(+) (pilA2) [Xanthomonas hortorum pv. vitians strain LM16734]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLVKARVSEVMLAASSARTAVSEAAAADTSATNMPATFTVETQASKYV
ASVAYVGGTTTGVITATAKDLGGSTPTGTITLTGTKAANGQVSWVCAGTIDVKYRPATCRGT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=475721 XHV734_RS05355 WP_180313578.1 1298977..1299405(+) (pilA2) [Xanthomonas hortorum pv. vitians strain LM16734]
ATGAAGAAGCAACAAGGTTTTACCCTGATCGAATTGATGATTGTGGTTGCGATCATCGCAATTCTGGCTGCCATCGCACT
GCCGGCTTATCAGGATTACCTGGTCAAGGCGCGCGTTTCGGAAGTGATGCTGGCCGCGTCTTCGGCACGTACGGCAGTTT
CGGAAGCTGCTGCTGCCGATACCAGTGCTACTAATATGCCCGCCACCTTTACTGTCGAGACACAGGCATCCAAGTATGTG
GCGAGTGTCGCCTATGTGGGTGGTACAACGACGGGTGTCATCACTGCAACCGCCAAGGATTTGGGTGGCAGTACTCCTAC
TGGAACAATCACACTTACTGGTACTAAGGCAGCCAACGGTCAGGTCTCCTGGGTGTGTGCTGGTACCATCGATGTGAAGT
ATCGTCCTGCTACCTGTCGCGGTACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6V7F519

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.448

100

0.528
  comP Acinetobacter baylyi ADP1

48.366

100

0.521
  pilA2 Legionella pneumophila strain ERS1305867

51.748

100

0.521
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.486
  pilA Ralstonia pseudosolanacearum GMI1000

40.606

100

0.472
  pilE Neisseria gonorrhoeae MS11

37.423

100

0.43
  pilA/pilA1 Eikenella corrodens VA1

39.474

100

0.423
  pilA Vibrio campbellii strain DS40M4

43.704

95.07

0.415
  pilA Pseudomonas aeruginosa PAK

37.419

100

0.408
  pilE Neisseria gonorrhoeae strain FA1090

35

100

0.394
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

100

0.38