Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XHV734_RS05360 Genome accession   NZ_CP060399
Coordinates   1299658..1301394 (+) Length   578 a.a.
NCBI ID   WP_187433766.1    Uniprot ID   -
Organism   Xanthomonas hortorum pv. vitians strain LM16734     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294658..1306394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XHV734_RS05335 (XHV734_1246) - 1294922..1295323 (-) 402 WP_074058084.1 SymE family type I addiction module toxin -
  XHV734_RS05340 (XHV734_1248) coaE 1295857..1296480 (-) 624 WP_154844506.1 dephospho-CoA kinase -
  XHV734_RS05345 (XHV734_1249) - 1296494..1297357 (-) 864 WP_002812278.1 A24 family peptidase -
  XHV734_RS05350 (XHV734_1250) pilC 1297364..1298623 (-) 1260 WP_180313588.1 type II secretion system F family protein Machinery gene
  XHV734_RS05355 (XHV734_1251) pilA2 1298977..1299405 (+) 429 WP_180313578.1 pilin Machinery gene
  XHV734_RS05360 (XHV734_1252) pilB 1299658..1301394 (+) 1737 WP_187433766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XHV734_RS05365 (XHV734_1253) pilR 1301736..1303190 (-) 1455 WP_174555994.1 sigma-54 dependent transcriptional regulator Regulator
  XHV734_RS05370 (XHV734_1254) - 1303276..1304889 (-) 1614 WP_006448368.1 HAMP domain-containing sensor histidine kinase -
  XHV734_RS05375 (XHV734_1255) sucC 1305122..1306291 (+) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62963.15 Da        Isoelectric Point: 5.4552

>NTDB_id=475722 XHV734_RS05360 WP_187433766.1 1299658..1301394(+) (pilB) [Xanthomonas hortorum pv. vitians strain LM16734]
MNAFSSVNLVGITGIARRLVQDGALEETVARSAMEQATLAKIPLPQWFAEKKLVSSAQLAAANAVEFGMPLLDVSSFDAS
QNAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQANSASFGSSMG
DDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTPEQVASGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=475722 XHV734_RS05360 WP_187433766.1 1299658..1301394(+) (pilB) [Xanthomonas hortorum pv. vitians strain LM16734]
ATGAATGCTTTCAGCTCAGTCAACCTTGTTGGCATAACCGGTATTGCTCGTCGCTTGGTTCAGGATGGGGCTTTAGAAGA
GACGGTTGCTCGGTCGGCCATGGAGCAGGCAACTCTTGCCAAAATCCCGTTGCCTCAATGGTTTGCTGAGAAGAAGTTGG
TTAGCAGTGCGCAGCTTGCCGCAGCAAATGCCGTTGAATTTGGCATGCCGTTGTTGGATGTGTCGTCTTTCGATGCTAGC
CAAAATGCGATCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAATACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACGCGTGCGCTGGACGACATCAAGTTCCACACCAATCTGGTTGTCGAGCCGA
TTTTGGTCGATGAAGATCAGATTCGTCGCACATTGGAGCAATGGCAGGCCAATAGCGCTTCGTTTGGCTCGTCAATGGGT
GACGATGACGAAGAAATGGGTGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGGGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGAGTGCGCTTGCGCATCGATGGGTTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAAAAGCTGGGCTACGAGGCGGATCAGCAGAAG
CTGTTTTTGGATGCGATCCACAAGCCCTACGGCATGGTATTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
ATATACGGCGCTGGGCATCCTCAATGATGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATTATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCAATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATTGCACCGTACAACATCA
CTTCGTCGGTGACCTTGGTCATTGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGGCACCGCTGCCG
GAGCACGCGCTGTTGGCCGAAGGCTTCACGCCAGAGCAGGTCGCTTCCGGCATCGAGCTATACGAAGCGGTTGGTTGCGA
TGAGTGCACAGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGC
TGGAAGGCGGTAATGCAATGCAGATCGCAGAGGCCGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGAGTGACGAGCCTGGCTGAGATCAATCGGGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.44

100

0.555
  pilB Acinetobacter baumannii D1279779

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

48.517

99.135

0.481
  pilF Neisseria gonorrhoeae MS11

48.944

98.27

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

47.048

96.713

0.455
  pilB Vibrio campbellii strain DS40M4

46.046

98.443

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.686

94.81

0.367
  pilF Thermus thermophilus HB27

40.115

90.138

0.362