Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BDGGKGIB_RS09470 Genome accession   NZ_CP060140
Coordinates   2345102..2346226 (-) Length   374 a.a.
NCBI ID   WP_239731502.1    Uniprot ID   -
Organism   Nodularia sphaerocarpa UHCC 0038     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2340102..2351226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BDGGKGIB_RS09450 - 2341837..2341980 (-) 144 WP_239731494.1 hypothetical protein -
  BDGGKGIB_RS09455 (BDGGKGIB_01917) bioU 2342145..2343149 (+) 1005 WP_239731496.1 (S)-8-amino-7-oxononanoate synthase BioU -
  BDGGKGIB_RS09460 (BDGGKGIB_01918) - 2343154..2343705 (-) 552 WP_239731498.1 hypothetical protein -
  BDGGKGIB_RS09465 (BDGGKGIB_01919) - 2343839..2345059 (-) 1221 WP_239731500.1 type II secretion system F family protein -
  BDGGKGIB_RS09470 (BDGGKGIB_01920) pilT 2345102..2346226 (-) 1125 WP_239731502.1 type IV pilus twitching motility protein PilT Machinery gene
  BDGGKGIB_RS09475 (BDGGKGIB_01921) pilB/pilB1 2346293..2348308 (-) 2016 WP_239731503.1 GspE/PulE family protein Machinery gene
  BDGGKGIB_RS09480 (BDGGKGIB_01922) grpE 2348806..2349552 (+) 747 WP_239731504.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41017.35 Da        Isoelectric Point: 8.2572

>NTDB_id=474116 BDGGKGIB_RS09470 WP_239731502.1 2345102..2346226(-) (pilT) [Nodularia sphaerocarpa UHCC 0038]
MEMMIEDLMEKLIEMGGSDMHLSAGLPPYFRISGKLTPIGDQVLSADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMSDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFIYEPIKSLVHQRQLNEDTKSFSNALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHEKQTQVRVQLSNSLVAIFSQTLVSKKNPKPGEFGRVMAQEILVVTPAISNLIREGKTSQIYSTIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEVQRLIGTSAPPAGTRPGAGATRAR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=474116 BDGGKGIB_RS09470 WP_239731502.1 2345102..2346226(-) (pilT) [Nodularia sphaerocarpa UHCC 0038]
ATGGAAATGATGATTGAAGACTTGATGGAAAAGTTGATTGAAATGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
CCCCTACTTTCGCATCAGTGGCAAATTGACCCCTATCGGTGACCAAGTATTGAGTGCAGATCAGTGCCAAAGGCTGATTT
TTAGTATGCTCAATAATACCCAGCGTAAAACCTTAGAACAAACTTGGGAGTTGGATTGTTCCTATGGCGTGAAGGGTTTA
GCTCGTTTTCGGGTGAATGTCTATAAAGAACGCGGTGCTTATGCTGCTTGTTTACGGGCGCTCAGTTCCAAAATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATAGTGCGGGAAATGTCCGATAAGCCCAGAGGATTAATTCTGGTGACAGGCCCCA
CAGGTTCCGGTAAGACAACCACCCTAGCGGCAATGATTGACTTAATTAACCGCACCAAAGCAGAGCATATTCTCACGGTG
GAAGATCCGATTGAATTTATTTATGAACCCATTAAAAGCCTAGTCCACCAAAGACAACTCAATGAAGATACCAAGAGTTT
TTCTAATGCTTTGAGGGCTGCTTTACGGGAAGATCCAGATATTATTCTGGTGGGGGAAATGCGTGATTTAGAAACAATTT
CTTTGGCTATTTCGGCGGCGGAAACAGGACACTTGGTATTTGGAACTTTACACACCAGTTCGGCTGCACAGACTGTTGAC
CGGATTATTGACGTGTTTCCCCATGAGAAACAAACTCAAGTGCGGGTGCAGTTATCTAACTCTTTAGTAGCCATATTTAG
CCAAACCTTGGTCAGCAAGAAAAACCCTAAACCTGGTGAATTTGGACGGGTAATGGCTCAAGAAATTCTCGTTGTTACTC
CTGCTATTTCTAACTTGATTCGAGAAGGGAAAACATCGCAAATTTACTCGACTATCCAGACTGGCGGTAAATTGGGGATG
CAAACTTTGGAAAAGGTTTTAGCTGATTTGTACAAAGCTGGAACTATTTCTTTTGAAGCTGCAATGTCTAAAACTTCCAA
GCCAGATGAAGTTCAACGTCTCATCGGGACTTCTGCACCACCAGCAGGTACAAGACCTGGGGCTGGCGCTACGAGAGCGC
GTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.755

92.781

0.508
  pilT Pseudomonas stutzeri DSM 10701

54.179

92.781

0.503
  pilT Legionella pneumophila strain Lp02

53.026

92.781

0.492
  pilT Legionella pneumophila strain ERS1305867

53.026

92.781

0.492
  pilT Acinetobacter baylyi ADP1

52.738

92.781

0.489
  pilT Acinetobacter baumannii strain A118

51.585

92.781

0.479
  pilT Acinetobacter baumannii D1279779

51.585

92.781

0.479
  pilT Acinetobacter nosocomialis M2

51.585

92.781

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

93.583

0.463
  pilT Neisseria gonorrhoeae MS11

50.147

90.642

0.455
  pilT Neisseria meningitidis 8013

50.147

90.642

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.444

0.452
  pilT Vibrio cholerae strain A1552

49.415

91.444

0.452