Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BDGGKGIB_RS09475 Genome accession   NZ_CP060140
Coordinates   2346293..2348308 (-) Length   671 a.a.
NCBI ID   WP_239731503.1    Uniprot ID   -
Organism   Nodularia sphaerocarpa UHCC 0038     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341293..2353308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BDGGKGIB_RS09450 - 2341837..2341980 (-) 144 WP_239731494.1 hypothetical protein -
  BDGGKGIB_RS09455 (BDGGKGIB_01917) bioU 2342145..2343149 (+) 1005 WP_239731496.1 (S)-8-amino-7-oxononanoate synthase BioU -
  BDGGKGIB_RS09460 (BDGGKGIB_01918) - 2343154..2343705 (-) 552 WP_239731498.1 hypothetical protein -
  BDGGKGIB_RS09465 (BDGGKGIB_01919) - 2343839..2345059 (-) 1221 WP_239731500.1 type II secretion system F family protein -
  BDGGKGIB_RS09470 (BDGGKGIB_01920) pilT 2345102..2346226 (-) 1125 WP_239731502.1 type IV pilus twitching motility protein PilT Machinery gene
  BDGGKGIB_RS09475 (BDGGKGIB_01921) pilB/pilB1 2346293..2348308 (-) 2016 WP_239731503.1 GspE/PulE family protein Machinery gene
  BDGGKGIB_RS09480 (BDGGKGIB_01922) grpE 2348806..2349552 (+) 747 WP_239731504.1 nucleotide exchange factor GrpE -
  BDGGKGIB_RS09485 (BDGGKGIB_01923) dnaK 2349844..2351808 (+) 1965 WP_239731506.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74833.64 Da        Isoelectric Point: 5.2388

>NTDB_id=474117 BDGGKGIB_RS09475 WP_239731503.1 2346293..2348308(-) (pilB/pilB1) [Nodularia sphaerocarpa UHCC 0038]
MTYSSPQRRSTALTTKTEFSPFGNKLVQSGYVNSEQMRLALIESRKSGKPLTEVLESITGKQLSPEFLRQYKKQQLFELK
ILYGVEFLDPEVNDIANPKVGNLIESLIPAEICRRHHLVPLSKNENHNPPSVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVIAQDDYQQLINQYLDDLALRQKQLDKEKLTDIHQDLENLSNIDIDDTPEEMEADLGAVMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLSEAFDPLPKKIVMAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHMVQDMVSRPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGLSASQNTGVTFYKANTLTLEEITAAKAKNQEVCPKCN
GSGYKGRCGVYEVMRITENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAK
RKSGLTCVSCNATLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=474117 BDGGKGIB_RS09475 WP_239731503.1 2346293..2348308(-) (pilB/pilB1) [Nodularia sphaerocarpa UHCC 0038]
ATGACTTATTCGTCACCACAACGGCGCAGTACCGCTCTAACTACAAAAACAGAGTTTTCGCCCTTCGGCAACAAGCTAGT
ACAGTCTGGCTATGTCAATAGCGAACAGATGAGGCTGGCGCTGATTGAAAGCCGCAAGTCTGGCAAACCCCTGACTGAAG
TCTTGGAGTCTATTACCGGAAAACAACTATCCCCTGAGTTTCTCAGACAATATAAAAAACAACAGCTCTTTGAACTGAAA
ATACTCTACGGTGTTGAGTTCCTAGATCCAGAAGTTAACGACATTGCCAACCCCAAAGTGGGCAACCTGATTGAAAGCCT
GATCCCGGCTGAAATTTGTCGTCGCCATCACTTGGTACCATTATCCAAAAATGAAAACCACAACCCACCCAGTGTCTTGG
TGGCAATGGTCGATCCGGATAATCTCGAAGCTTCCGATGATCTCAACCGCATCCTACGCCCCCAAGGTTTGGCATTACAA
CGCATGGTAATTGCCCAAGACGATTACCAACAGCTAATTAACCAATATTTGGATGACTTAGCTCTGCGCCAAAAGCAGCT
GGACAAAGAAAAGTTGACAGATATTCATCAAGATTTAGAAAATCTGAGTAATATCGACATAGACGATACCCCCGAAGAGA
TGGAGGCTGATTTAGGGGCAGTCATGAAGGGTGCGGAAGATGCCCCAGTGATTAACCTTGTCAACAGAATTCTGGCCAAA
GCCTTACATGAGGGTGTTTCTGATATTCACATCGAACCACAGGAGGAAAATTTAAGGATTCGGTTTCGTAAGGATGGGGT
ATTAAGTGAAGCTTTCGACCCCCTACCCAAAAAAATCGTGATGGCGGTGACAGCTCGATTTAAAATCATCTCTAATCTAG
ACATTGCAGAGCGACGCTTACCCCAAGATGGACGCATCCGGCGACTGTTCGAGGGACGTAAGGTGGACTTCCGGGTGAAT
ACTTTGCCTAGTCGCTATGGGGAAAAAGTAGTACTGCGGATTTTGGATAACTCTTCCACGCAGTTGGGATTAGATAAGTT
GATTACTGATCCTGAAACTCTGCACATGGTTCAGGATATGGTCAGCCGTCCTTTTGGGTTGATTTTGGTGACTGGGCCAA
CGGGTTCTGGTAAAACTACATCGCTATATTCGGCATTAGCAGAAAAAAACTCGCCGGGGATTAATATTAGTACAGTAGAA
GACCCCATTGAGTACAGCTTGCCAGGGATTACTCAAGTACAAGTCATTCGGGAAAAAGGGTTAGATTTTTCTACGGCTTT
GCGGGCGTTTTTGCGGCAAGACCCGGATGTGCTGCTAGTGGGTGAAACACGGGACAAAGAAACTGCCAAAACCGCCATTG
AGGCTGCTTTAACTGGCCACTTAGTATTAACTACTTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTCTGGGAGAA
ATGGGTATTGAGCCTTTTATGGTTTCTAGTTCCCTGATTGGTGTGTTGGCTCAACGTTTGGTGCGGCGTGTCTGTTCTGA
ATGTCGTATTGCCTATACTCCCACAACTGAGGAACTAGCTCGCTATGGCCTCTCAGCTTCCCAAAATACAGGTGTAACTT
TTTACAAAGCTAACACTTTAACATTAGAAGAAATTACGGCAGCAAAAGCCAAAAATCAGGAAGTTTGTCCCAAGTGTAAT
GGCTCTGGCTACAAAGGACGTTGTGGTGTTTATGAAGTGATGCGAATCACGGAAAACCTGCAAACTCTGATTAACCAAGA
AGCACCAACAGAACGCATTAAGGAAGTAGCAGTAGAAGAAGGCATGAAAACCTTGCTGGCTTACAGTCTGGACTTAGTGC
GTCAGGGTGCTACGACTCTCGAAGAAGTAGAACGAGTCACCTTTACTGATACCGGCTTGGAAGCCGAGTTAAAAGCCAAA
CGCAAGAGTGGTCTGACTTGTGTGAGTTGTAATGCAACATTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACACC
TCGTTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.973

100

0.662
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.803

90.611

0.361