Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GWQ54_RS19990 Genome accession   NZ_CP070373
Coordinates   4556720..4557832 (+) Length   370 a.a.
NCBI ID   WP_240361778.1    Uniprot ID   -
Organism   Streptomyces sp. MBT27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4551720..4562832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWQ54_RS19975 - 4551962..4552390 (+) 429 WP_167161173.1 type II secretion system protein -
  GWQ54_RS19980 - 4552387..4552911 (+) 525 WP_167161172.1 type II secretion system protein -
  GWQ54_RS19985 - 4552908..4556723 (+) 3816 WP_205530685.1 hypothetical protein -
  GWQ54_RS19990 pilT 4556720..4557832 (+) 1113 WP_240361778.1 type IV pilus twitching motility protein PilT Machinery gene
  GWQ54_RS19995 - 4557894..4559087 (-) 1194 WP_167161171.1 amidohydrolase family protein -
  GWQ54_RS20000 - 4559125..4559898 (+) 774 WP_167161170.1 NAD(P)H-binding protein -
  GWQ54_RS20005 - 4559895..4560989 (+) 1095 WP_167161169.1 hypothetical protein -
  GWQ54_RS20010 - 4561285..4561569 (+) 285 WP_167161168.1 hypothetical protein -
  GWQ54_RS20015 - 4561572..4561727 (+) 156 WP_167161167.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40163.91 Da        Isoelectric Point: 6.7831

>NTDB_id=474063 GWQ54_RS19990 WP_240361778.1 4556720..4557832(+) (pilT) [Streptomyces sp. MBT27]
MRKHTPLEATMSGPSAATIDHLLVELWKRGGSDLLLTTGSAPFLRIDGALRPVEGAPLTEPEVDRLVTGVLGEELTERFR
REKQVDFAFSWGQKARLRGNAFVQRGASALALRIIPFDVPSSTQLGLPPAAIGWAGLPRGFVLVTGPTGSGKSTSLAALL
DYVNTHRSVHILTIEDPIEYLHQHKRSAVNQREVGTDTDSFPAALRSALREDPDVLLLGEMRDPESISAALTIAETGHLV
FATLHTNDTSQTLDRIVDVFPAEQQPQIRLQLAHTLVGILNQTLIPRIGGGRVAAFEVMVGTSAIRNLIREGKTRQIRNM
IATGHRDGMQTLETNINALVAAGTIAYEEAVLCTAHPEDIQRPHVPVGRI

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=474063 GWQ54_RS19990 WP_240361778.1 4556720..4557832(+) (pilT) [Streptomyces sp. MBT27]
ATGAGGAAGCACACCCCGTTGGAGGCAACCATGTCCGGGCCAAGTGCCGCGACCATTGACCACCTGCTCGTCGAGCTGTG
GAAGCGGGGCGGCAGCGACCTGCTGCTCACCACCGGATCGGCGCCGTTCCTCCGCATCGACGGCGCTCTGCGACCGGTCG
AGGGGGCGCCTCTCACCGAGCCGGAGGTCGATCGCCTGGTTACCGGGGTGCTGGGCGAGGAACTCACGGAACGGTTCCGC
CGCGAGAAGCAGGTCGACTTCGCCTTCAGCTGGGGGCAGAAGGCCAGGTTGCGTGGGAACGCTTTCGTGCAGCGCGGCGC
GTCGGCGCTGGCTCTGCGGATCATCCCGTTCGACGTGCCGTCGTCCACGCAGCTCGGGCTCCCCCCGGCCGCCATCGGGT
GGGCAGGCCTGCCGCGCGGGTTCGTGCTGGTCACCGGGCCGACCGGGTCGGGCAAGTCGACGAGCCTGGCGGCGCTCCTC
GATTACGTCAACACACACCGGTCCGTGCACATCCTCACCATCGAGGACCCGATCGAGTATCTGCACCAGCACAAGCGCTC
GGCGGTCAACCAGCGCGAGGTCGGCACGGACACCGACTCGTTCCCCGCCGCGTTGCGCTCGGCCCTGCGCGAGGATCCCG
ACGTCCTGCTCCTCGGCGAGATGCGCGACCCGGAGAGCATCTCCGCGGCCCTGACCATCGCCGAGACCGGGCACCTCGTG
TTCGCCACCCTGCACACCAACGATACGTCGCAGACGCTGGACCGGATCGTCGACGTGTTCCCGGCCGAGCAACAGCCGCA
AATCCGGCTGCAATTGGCGCACACTCTGGTCGGCATCCTCAACCAGACGCTCATCCCCCGGATCGGCGGGGGCCGAGTGG
CCGCCTTCGAGGTGATGGTAGGCACGTCGGCGATCCGCAACCTCATCCGCGAAGGCAAGACCCGTCAGATCCGCAACATG
ATCGCGACCGGCCACCGTGACGGAATGCAGACCCTTGAGACCAACATCAACGCCTTGGTCGCGGCAGGCACCATCGCCTA
CGAGGAGGCCGTGCTGTGCACGGCACACCCGGAGGACATCCAGCGGCCCCATGTCCCGGTCGGCCGCATCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(138-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.729

92.703

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.98

92.703

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.278

94.324

0.446
  pilT Acinetobacter baumannii strain A118

48.529

91.892

0.446
  pilT Acinetobacter baumannii D1279779

48.529

91.892

0.446
  pilT Acinetobacter nosocomialis M2

48.235

91.892

0.443
  pilT Neisseria meningitidis 8013

48.204

90.27

0.435
  pilT Acinetobacter baylyi ADP1

47.353

91.892

0.435
  pilT Legionella pneumophila strain Lp02

48.494

89.73

0.435
  pilT Legionella pneumophila strain ERS1305867

48.494

89.73

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.536

87.297

0.432
  pilT Vibrio cholerae strain A1552

49.536

87.297

0.432
  pilT Neisseria gonorrhoeae MS11

47.447

90

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.159

93.243

0.384