Detailed information NTG0453982

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HUN01_RS33500
• Genome accession: NZ_CP054698
• Coordinates: 7401545..7402675 (-)
• Length: 376 a.a.
• NCBI ID: WP_181929769.1
• Uniprot ID: A0A7D7R9W9
• Organism: Nostoc edaphicum CCNP1411
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 7396545..7407675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUN01_RS33485 (HUN01_33485)	-	7397822..7398886 (-)	1065	WP_181929766.1	serine/threonine-protein kinase	-
HUN01_RS33490 (HUN01_33490)	-	7399523..7400083 (-)	561	WP_181929767.1	hypothetical protein	-
HUN01_RS33495 (HUN01_33495)	-	7400225..7401448 (-)	1224	WP_181929768.1	type II secretion system F family protein	-
HUN01_RS33500 (HUN01_33500)	pilT	7401545..7402675 (-)	1131	WP_181929769.1	type IV pilus twitching motility protein PilT	Machinery gene
HUN01_RS33505 (HUN01_33505)	pilB/pilB1	7402821..7404824 (-)	2004	WP_181929770.1	GspE/PulE family protein	Machinery gene
HUN01_RS33510 (HUN01_33510)	grpE	7405183..7405929 (+)	747	WP_181929771.1	nucleotide exchange factor GrpE	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41158.37 Da
• Isoelectric Point: 7.1087

>NTDB_id=453982 HUN01_RS33500 WP_181929769.1 7401545..7402675(-) (pilT) [Nostoc edaphicum CCNP1411]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEDVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPNEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGNSTPQGAAAGAKPGATAKAH

Nucleotide

• Length: 1131 bp

>NTDB_id=453982 HUN01_RS33500 WP_181929769.1 7401545..7402675(-) (pilT) [Nostoc edaphicum CCNP1411]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGCTGATTGAATTGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTATTTCCGGATCAGTGGCAAACTCACTCCCATAGGTGAGGATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAATAACACCCAGCGTAAAACTCTAGAGCAAAACTGGGAGTTGGATTGTTCTTATGGTGTTAAGGGTTTA
GCTCGCTTCCGGGTCAATGTCTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATCGTGCGGGAAATGACAGATAAGCCCAGAGGGCTAATTCTGGTAACAGGCCCTA
CAGGTTCAGGTAAGACAACTACCCTAGCGGCAATGATTGACTTGATTAACCGCACCAAGGCAGAGCATATTTTGACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCAATAAAAAGCTTGGTTCACCAGCGGCAACTGGGGGAAGATACTAAGAGCTT
TGCTAATGCTTTGAAAGCAGCTTTGCGGGAAGACCCAGACATCGTTCTGGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCAATTTCTGCTGCGGAAACAGGACACTTGGTATTTGGGACGCTACACACCAGTTCTGCTGCACAGACAGTTGAC
CGGATTATTGACGTTTTCCCTCATGAAAGACAAACCCAAGTGCGGGTGCAGTTGTCTAACTCCTTAGTGGCGGTATTTAG
CCAAACCTTGGTGTCTAAGAAAAACCCTAAACCCAATGAATATGGCCGGGTGATGGCTCAAGAAATTCTGATTGTTACTC
CTGCTATTTCTAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGAATG
CAAACTCTGGAGAAGGTTTTAGCCGATTTTTACAAAGCAGGAACGATTTCTTTTGAAGCGGCGATGTCTAAAACTTCTAA
GCCAGATGAAATTCAACGTCTCATTGGTAATTCTACACCGCAAGGAGCTGCCGCAGGTGCGAAACCCGGTGCGACTGCCA
AAGCGCATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (44-271)

3D structure

• AlphaFold DB: A0A7D7R9W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas aeruginosa PAK	54.467	92.287	0.503
pilT	Pseudomonas stutzeri DSM 10701	54.179	92.287	0.5
pilT	Acinetobacter baylyi ADP1	52.738	92.287	0.487
pilT	Legionella pneumophila strain ERS1305867	52.45	92.287	0.484
pilT	Legionella pneumophila strain Lp02	52.45	92.287	0.484
pilT	Acinetobacter nosocomialis M2	52.161	92.287	0.481
pilT	Acinetobacter baumannii D1279779	52.161	92.287	0.481
pilT	Acinetobacter baumannii strain A118	52.161	92.287	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.411	97.606	0.463
pilT	Neisseria gonorrhoeae MS11	50.737	90.16	0.457
pilT	Neisseria meningitidis 8013	50.442	90.16	0.455
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.415	90.957	0.449
pilT	Vibrio cholerae strain A1552	49.415	90.957	0.449