Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HUN01_RS33505 Genome accession   NZ_CP054698
Coordinates   7402821..7404824 (-) Length   667 a.a.
NCBI ID   WP_181929770.1    Uniprot ID   A0A7D7LLE5
Organism   Nostoc edaphicum CCNP1411     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7397821..7409824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUN01_RS33485 (HUN01_33485) - 7397822..7398886 (-) 1065 WP_181929766.1 serine/threonine-protein kinase -
  HUN01_RS33490 (HUN01_33490) - 7399523..7400083 (-) 561 WP_181929767.1 hypothetical protein -
  HUN01_RS33495 (HUN01_33495) - 7400225..7401448 (-) 1224 WP_181929768.1 type II secretion system F family protein -
  HUN01_RS33500 (HUN01_33500) pilT 7401545..7402675 (-) 1131 WP_181929769.1 type IV pilus twitching motility protein PilT Machinery gene
  HUN01_RS33505 (HUN01_33505) pilB/pilB1 7402821..7404824 (-) 2004 WP_181929770.1 GspE/PulE family protein Machinery gene
  HUN01_RS33510 (HUN01_33510) grpE 7405183..7405929 (+) 747 WP_181929771.1 nucleotide exchange factor GrpE -
  HUN01_RS33515 (HUN01_33515) dnaK 7406157..7408115 (+) 1959 WP_181929772.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74426.25 Da        Isoelectric Point: 5.1727

>NTDB_id=453983 HUN01_RS33505 WP_181929770.1 7402821..7404824(-) (pilB/pilB1) [Nostoc edaphicum CCNP1411]
MNSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELKI
LYGVEFLDPEVNSVGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQR
MVITQEDYQQLINQYLDEMAVKQKHLEQEKFTDINQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAKA
LHEGVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVGVTFYKANTLTLDAIADAKAKNQLCSKCNGI
GYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKRK
TGLTCRSCDATLKQEWLDCPYCMTPRF

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=453983 HUN01_RS33505 WP_181929770.1 7402821..7404824(-) (pilB/pilB1) [Nostoc edaphicum CCNP1411]
ATGAACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGTGCA
ATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCATTAATTGAAAGCCGCAAATCTGGCAGACCCTTAACGGAAGTCT
TAGAGTCAATCACTGGGCAACAACTATCGCCTGAGTTGCTTAGGCAATACAAAAAACAACAGCTATTTGAACTGAAAATA
CTATACGGAGTTGAATTCCTTGATCCGGAAGTCAACTCCGTTGGCAACACGATGATGGGGAACCTGATTGAAACCCTCAT
CCCAGTGGATATCTGTCGTCGTCATCGTTTAGTACCACTCTCGAAACACGAAGACCAAACCCCACCCTCAGTTTTAGTGG
CGATGGTCGCTCCAGATAATCTAGAGGCTTCCGATGACCTGAACCGCATTTTGCGCCCCCAAGGCTTGGCGTTGCAGCGC
ATGGTGATTACCCAGGAAGACTATCAACAGCTAATCAACCAATATCTGGATGAAATGGCTGTTAAGCAGAAGCACCTGGA
ACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTCGATCTTGCAGATGCTCCTGAAGAAATGG
AGGCTGATCTAGGGGCAGCGATGAAGGGTGCAGAGGATGCCCCAGTCATCAACTTAGTTAACAGAATCCTGGCTAAAGCT
TTGCATGAGGGCGTTTCTGATATTCATATAGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGCAAGGATGGCGTATT
GCGTGAAGCTTTTGATCCCCTACCGAAAAAAATCATCCCAGCGGTGACAGCCCGATTTAAGATCATCTCCAATCTAGACA
TTGCTGAACGCCGTCTACCCCAGGATGGACGCATCCGGCGGATGTTTGAGGGACGCAAAGTGGATTTCCGGGTGAATACC
TTACCCAGTCGCTACGGGGAAAAGGTGGTACTGCGGATTTTGGATAACTCCTCCACCCAACTAGGATTAGACAAGTTAAT
TACCGATCCAGAGACTTTACATATTGTTCAGGATATGGTTAGCCGTCCCTTTGGGCTAATTTTAGTAACTGGGCCAACTG
GTTCTGGGAAAACGACCTCGCTATATTCTGCACTCTCAGAAAAGAATGATCCTGGAATTAATATCAGTACTGTGGAAGAC
CCAATTGAGTACAGCCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTAGATTTTGCGACTGCGTTGCG
GGCTTTCTTGCGACAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAAGAAACGGCAAAAACAGCAATTGAGG
CTGCCTTGACCGGTCACTTAGTATTAACTACCTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAAATG
GGGATTGAGCCTTTTATGGTTTCTAGTTCCCTAATTGGCGTTCTCGCTCAACGTCTGGTGCGGCGTGTATGTTCTGAATG
TCGCATTCCTTACACTCCTACAACCGAGGAACTAGCTCGTTATGGTCTATCAGCTTCCTCTGATGTGGGAGTCACCTTCT
ACAAAGCTAACACCTTGACATTAGATGCGATCGCAGATGCCAAAGCCAAAAATCAACTTTGCTCAAAATGTAATGGTATC
GGCTACAAAGGGCGTTGTGGTGTTTATGAAGTTATGCGAGTCACCGAAAACCTGCAAACTCTCATCAACGAAGATGCACC
CACGGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACCTTACTGGCTTACAGTTTGGACTTAGTGCGTGAAG
GTTCCACAACTCTAGAAGAAGTAGAACGGGTGACGTTTACCGATACTGGTTTGGAAGCTGAGTTAAAAGCCAAACGCAAG
ACTGGTCTTACCTGTCGGAGTTGCGATGCCACATTGAAACAAGAATGGCTGGATTGTCCTTACTGTATGACACCTCGGTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(63-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D7LLE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.566

99.1

0.66
  pilF Thermus thermophilus HB27

39.315

91.904

0.361