Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HND92_RS02525 Genome accession   NZ_CP053553
Coordinates   514594..515730 (-) Length   378 a.a.
NCBI ID   WP_011803879.1    Uniprot ID   A0A6M6IC05
Organism   Diaphorobacter sp. JS3050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509594..520730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HND92_RS02495 (HND92_02495) - 509719..510647 (+) 929 Protein_500 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  HND92_RS02500 (HND92_02500) rsmI 510843..511781 (-) 939 WP_172205642.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  HND92_RS02505 (HND92_02505) - 511781..512179 (+) 399 WP_011803875.1 YraN family protein -
  HND92_RS02510 (HND92_02510) - 512238..512831 (+) 594 WP_172205644.1 SIS domain-containing protein -
  HND92_RS02515 (HND92_02515) - 512828..513502 (+) 675 WP_012655453.1 BON domain-containing protein -
  HND92_RS02520 (HND92_02520) - 513600..514529 (-) 930 WP_011803878.1 NAD(P)-dependent oxidoreductase -
  HND92_RS02525 (HND92_02525) pilU 514594..515730 (-) 1137 WP_011803879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HND92_RS02530 (HND92_02530) - 515777..516406 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  HND92_RS02535 (HND92_02535) pilT 516483..517526 (-) 1044 WP_011803881.1 type IV pilus twitching motility protein PilT Machinery gene
  HND92_RS02540 (HND92_02540) - 517566..518282 (+) 717 WP_047350273.1 YggS family pyridoxal phosphate-dependent enzyme -
  HND92_RS02545 (HND92_02545) - 518321..519457 (+) 1137 WP_012655458.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41715.16 Da        Isoelectric Point: 6.9080

>NTDB_id=445097 HND92_RS02525 WP_011803879.1 514594..515730(-) (pilU) [Diaphorobacter sp. JS3050]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGVGRFRVNAFVQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMLDWRNENSFG
HIITVEDPVEFVHPHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVISYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=445097 HND92_RS02525 WP_011803879.1 514594..515730(-) (pilU) [Diaphorobacter sp. JS3050]
ATGGAACGGGATCAGGCGAGTAAATTCATCAACGATCTGCTCAAGCTGATGGTCAGCCGTGGGGGGAGCGACCTTTTCAT
CACGGCGGAGTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCTCCCCAGCCTCTGACGCCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTTGCCATC
TCGCCTGCGGGCGTGGGCCGTTTCCGCGTGAACGCCTTCGTGCAGCAGGGCCGCGTGGGCATGGTGCTGCGGACGATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTTCCGCAGGTGCTCAAGGAAGTCACCATGACCAAGCGCGGATTGTGCA
TTTTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGCTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCAGTGGAATTCGTGCATCCGCACAAGAACTGCGTGATCACGCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCGGCCCTCAAGAACACGCTGCGTCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGCACTGGTCTCGCAGCGCCTGATTCCCAAGCAGGACGGCAAGGGGCGCGCGGCCGCCGTCGAGGTGATGCTGAACA
CGCCGCTGATCGCCGACCTCATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGCCAACGTGATCAGCTATGAGGATGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCTTGCAGATCAAGCTCAGCAGCCAGCGTGCCAAGACGACGGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6IC05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

58.659

94.709

0.556
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Acinetobacter baumannii strain A118

45.646

88.095

0.402
  pilT Acinetobacter nosocomialis M2

45.646

88.095

0.402
  pilT Acinetobacter baumannii D1279779

45.646

88.095

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368