Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   HKM25_RS10345 Genome accession   NZ_CP053210
Coordinates   2011014..2011955 (-) Length   313 a.a.
NCBI ID   WP_000249555.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 6A-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2006014..2016955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKM25_RS10305 (HKM25_2111) comYH 2006501..2007454 (-) 954 WP_000345121.1 class I SAM-dependent methyltransferase Machinery gene
  HKM25_RS10310 - 2007515..2008108 (-) 594 Protein_2026 methyltransferase domain-containing protein -
  HKM25_RS10315 (HKM25_2114) comGG/cglG 2008245..2008658 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  HKM25_RS10320 (HKM25_2115) comGF/cglF 2008636..2009097 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  HKM25_RS10325 (HKM25_2116) comGE/cglE 2009060..2009362 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  HKM25_RS10330 (HKM25_2117) comGD/cglD 2009325..2009729 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  HKM25_RS10335 (HKM25_2118) comGC/cglC 2009722..2010048 (-) 327 WP_000738635.1 comG operon protein ComGC Machinery gene
  HKM25_RS10340 (HKM25_2119) comGB/cglB 2010050..2011066 (-) 1017 WP_078151629.1 competence type IV pilus assembly protein ComGB Machinery gene
  HKM25_RS10345 (HKM25_2120) comGA/cglA/cilD 2011014..2011955 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  HKM25_RS10350 (HKM25_2121) - 2012031..2012396 (-) 366 WP_000286415.1 DUF1033 family protein -
  HKM25_RS10355 (HKM25_2122) - 2012547..2013605 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  HKM25_RS10360 (HKM25_2123) nagA 2013768..2014919 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -
  HKM25_RS10365 (HKM25_2124) - 2015072..2016889 (-) 1818 WP_001220922.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.49 Da        Isoelectric Point: 6.0083

>NTDB_id=443479 HKM25_RS10345 WP_000249555.1 2011014..2011955(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 6A-10]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=443479 HKM25_RS10345 WP_000249555.1 2011014..2011955(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 6A-10]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGATATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364