Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   HKM25_RS10340 Genome accession   NZ_CP053210
Coordinates   2010050..2011066 (-) Length   338 a.a.
NCBI ID   WP_078151629.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 6A-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2005050..2016066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKM25_RS11980 (HKM25_2109) - 2005128..2005259 (-) 132 WP_000768904.1 hypothetical protein -
  HKM25_RS10300 (HKM25_2110) - 2005260..2006450 (-) 1191 WP_054362660.1 acetate kinase -
  HKM25_RS10305 (HKM25_2111) comYH 2006501..2007454 (-) 954 WP_000345121.1 class I SAM-dependent methyltransferase Machinery gene
  HKM25_RS10310 - 2007515..2008108 (-) 594 Protein_2026 methyltransferase domain-containing protein -
  HKM25_RS10315 (HKM25_2114) comGG/cglG 2008245..2008658 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  HKM25_RS10320 (HKM25_2115) comGF/cglF 2008636..2009097 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  HKM25_RS10325 (HKM25_2116) comGE/cglE 2009060..2009362 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  HKM25_RS10330 (HKM25_2117) comGD/cglD 2009325..2009729 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  HKM25_RS10335 (HKM25_2118) comGC/cglC 2009722..2010048 (-) 327 WP_000738635.1 comG operon protein ComGC Machinery gene
  HKM25_RS10340 (HKM25_2119) comGB/cglB 2010050..2011066 (-) 1017 WP_078151629.1 competence type IV pilus assembly protein ComGB Machinery gene
  HKM25_RS10345 (HKM25_2120) comGA/cglA/cilD 2011014..2011955 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  HKM25_RS10350 (HKM25_2121) - 2012031..2012396 (-) 366 WP_000286415.1 DUF1033 family protein -
  HKM25_RS10355 (HKM25_2122) - 2012547..2013605 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  HKM25_RS10360 (HKM25_2123) nagA 2013768..2014919 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38508.56 Da        Isoelectric Point: 9.6162

>NTDB_id=443478 HKM25_RS10340 WP_078151629.1 2010050..2011066(-) (comGB/cglB) [Streptococcus pneumoniae strain 6A-10]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMILQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=443478 HKM25_RS10340 WP_078151629.1 2010050..2011066(-) (comGB/cglB) [Streptococcus pneumoniae strain 6A-10]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGATAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTTACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503