Detailed information NTG0443474

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: HKM25_RS10320
• Genome accession: NZ_CP053210
• Coordinates: 2008636..2009097 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250545.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain 6A-10
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2003636..2014097

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKM25_RS10290 (HKM25_2107)	-	2003941..2004765 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
HKM25_RS10295 (HKM25_2108)	rnpA	2004740..2005111 (-)	372	WP_000739246.1	ribonuclease P protein component	-
HKM25_RS11980 (HKM25_2109)	-	2005128..2005259 (-)	132	WP_000768904.1	hypothetical protein	-
HKM25_RS10300 (HKM25_2110)	-	2005260..2006450 (-)	1191	WP_054362660.1	acetate kinase	-
HKM25_RS10305 (HKM25_2111)	comYH	2006501..2007454 (-)	954	WP_000345121.1	class I SAM-dependent methyltransferase	Machinery gene
HKM25_RS10310	-	2007515..2008108 (-)	594	Protein_2026	methyltransferase domain-containing protein	-
HKM25_RS10315 (HKM25_2114)	comGG/cglG	2008245..2008658 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
HKM25_RS10320 (HKM25_2115)	comGF/cglF	2008636..2009097 (-)	462	WP_000250545.1	competence type IV pilus minor pilin ComGF	Machinery gene
HKM25_RS10325 (HKM25_2116)	comGE/cglE	2009060..2009362 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
HKM25_RS10330 (HKM25_2117)	comGD/cglD	2009325..2009729 (-)	405	WP_000588005.1	competence type IV pilus minor pilin ComGD	Machinery gene
HKM25_RS10335 (HKM25_2118)	comGC/cglC	2009722..2010048 (-)	327	WP_000738635.1	comG operon protein ComGC	Machinery gene
HKM25_RS10340 (HKM25_2119)	comGB/cglB	2010050..2011066 (-)	1017	WP_078151629.1	competence type IV pilus assembly protein ComGB	Machinery gene
HKM25_RS10345 (HKM25_2120)	comGA/cglA/cilD	2011014..2011955 (-)	942	WP_000249555.1	competence type IV pilus ATPase ComGA	Machinery gene
HKM25_RS10350 (HKM25_2121)	-	2012031..2012396 (-)	366	WP_000286415.1	DUF1033 family protein	-
HKM25_RS10355 (HKM25_2122)	-	2012547..2013605 (-)	1059	WP_000649466.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17855.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=443474 HKM25_RS10320 WP_000250545.1 2008636..2009097(-) (comGF/cglF) [Streptococcus pneumoniae strain 6A-10]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=443474 HKM25_RS10320 WP_000250545.1 2008636..2009097(-) (comGF/cglF) [Streptococcus pneumoniae strain 6A-10]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
TGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915