Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH213_RS15970 Genome accession   NZ_CP051684
Coordinates   3502029..3503210 (+) Length   393 a.a.
NCBI ID   WP_110847337.1    Uniprot ID   -
Organism   Duganella dendranthematis strain AF9R3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3497029..3508210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH213_RS15945 (HH213_15945) - 3498008..3498721 (+) 714 WP_169115217.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH213_RS15950 (HH213_15950) proC 3498725..3499546 (+) 822 WP_169112862.1 pyrroline-5-carboxylate reductase -
  HH213_RS15955 (HH213_15955) - 3499652..3499987 (-) 336 WP_110847340.1 YqjD family protein -
  HH213_RS15960 (HH213_15960) ubiA 3500083..3500937 (-) 855 WP_169112863.1 4-hydroxybenzoate octaprenyltransferase -
  HH213_RS15965 (HH213_15965) - 3500993..3501928 (-) 936 WP_161057424.1 hydrogen peroxide-inducible genes activator -
  HH213_RS15970 (HH213_15970) pilU 3502029..3503210 (+) 1182 WP_110847337.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH213_RS15975 (HH213_15975) rfaE2 3503220..3503702 (+) 483 WP_169112864.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  HH213_RS15980 (HH213_15980) - 3503699..3503989 (+) 291 WP_110847335.1 hypothetical protein -
  HH213_RS15985 (HH213_15985) - 3503986..3505059 (+) 1074 WP_229263024.1 helix-turn-helix domain-containing protein -
  HH213_RS15990 (HH213_15990) - 3505056..3505475 (-) 420 WP_169112865.1 phage tail protein -
  HH213_RS15995 (HH213_15995) - 3505482..3506330 (-) 849 WP_169112866.1 phage tail protein -
  HH213_RS16000 (HH213_16000) - 3506519..3507265 (+) 747 WP_169112867.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43379.63 Da        Isoelectric Point: 6.2885

>NTDB_id=440440 HH213_RS15970 WP_110847337.1 3502029..3503210(+) (pilU) [Duganella dendranthematis strain AF9R3]
MSSTFGPEEAQAYLHKLLKVMHHTGGSDLFIAADFPPSIKAQGAMKPLSQQKLTGSVTRALALSIMNDKQQREFETELEC
NFAISLPEVCRFRVNVFVQQQNVGMVIRTIASEIPTLEKLELPEVLKDIVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGNFQSIKEIMQKS
RELGMCTFDQALYELYNKGYIAYEEALRNADSANGLRLQIKLHGGRAEPGEGGGAFGALSIEQDAAPDDESKT

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=440440 HH213_RS15970 WP_110847337.1 3502029..3503210(+) (pilU) [Duganella dendranthematis strain AF9R3]
ATGTCCTCAACATTTGGTCCTGAAGAAGCCCAAGCCTATCTGCACAAGCTGCTGAAGGTGATGCACCACACCGGCGGTTC
GGACTTGTTTATCGCCGCCGACTTCCCGCCCAGTATCAAGGCGCAAGGGGCGATGAAGCCGTTGAGCCAGCAGAAGCTGA
CCGGCAGCGTGACGCGCGCGCTGGCGTTGTCGATCATGAACGACAAGCAGCAGCGCGAGTTCGAGACTGAGCTGGAATGC
AATTTTGCCATCTCGCTGCCGGAGGTGTGCCGGTTCCGCGTCAACGTCTTCGTGCAGCAACAGAATGTGGGCATGGTGAT
CCGTACCATCGCTTCCGAGATCCCGACGCTGGAAAAGCTGGAACTGCCGGAGGTGCTGAAGGACATCGTCATGACCAAGC
GCGGGCTGGTACTGGTGGTGGGTGGCACCGGTTCCGGCAAGTCGACCACGCTGGCGGCGATGATCGATTACCGCAACGCG
AATTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAGTACGTGCACAAGAACAAGGGCTGCCTGGTCACACACCG
CGAGGTCGGCGTTGATACGCTGTCGTGGCACAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAAACCATGGAGCACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGGCACGCTGCAC
GCCAACAACGCCAACCAGACCATGGACCGTATCATCAATTTCTTCCCGGAAGAGCGGCGCAACCAGCTGCTGATGGATTT
GTCGTCCAACCTGCGCGCCATCGTTTCACAGCGGCTGATCCGCACCGAAGACGGCAAGGGCCGCAAGGCGGCGATTGAAA
TCCTGCTCAACACGCCGACCATCGCCGAGATGATTTTCAAAGGGAATTTCCAGAGCATCAAGGAGATCATGCAGAAATCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCGCTGTATGAACTTTATAATAAAGGCTATATTGCCTACGAAGAGGCGCT
GCGCAATGCCGACTCGGCCAATGGCCTGCGCCTGCAGATCAAGCTGCACGGCGGCCGCGCCGAACCGGGCGAGGGCGGCG
GCGCCTTTGGCGCGCTGAGCATCGAACAAGACGCAGCACCAGACGACGAATCCAAAACCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.172

88.804

0.534
  pilU Acinetobacter baylyi ADP1

55.331

88.295

0.489
  pilU Vibrio cholerae strain A1552

51.705

89.567

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.509

84.987

0.387
  pilT Pseudomonas aeruginosa PAK

45.075

85.242

0.384
  pilT Legionella pneumophila strain ERS1305867

45.399

82.952

0.377
  pilT Legionella pneumophila strain Lp02

45.399

82.952

0.377
  pilT Pseudomonas stutzeri DSM 10701

44.179

85.242

0.377
  pilT Acinetobacter baylyi ADP1

46.835

80.407

0.377
  pilT Acinetobacter baumannii D1279779

44.411

84.224

0.374
  pilT Acinetobacter baumannii strain A118

44.411

84.224

0.374
  pilT Acinetobacter nosocomialis M2

44.411

84.224

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.312

81.425

0.369
  pilT Vibrio cholerae strain A1552

45.312

81.425

0.369