Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G6R31_RS01535 Genome accession   NZ_CP049357
Coordinates   296113..297375 (+) Length   420 a.a.
NCBI ID   WP_114671147.1    Uniprot ID   -
Organism   Deinococcus wulumuqiensis R12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291113..302375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6R31_RS01520 (G6R31_01520) pgeF 291921..292670 (+) 750 WP_017870160.1 peptidoglycan editing factor PgeF -
  G6R31_RS01525 (G6R31_01525) - 292667..293176 (+) 510 WP_017870161.1 YqeG family HAD IIIA-type phosphatase -
  G6R31_RS01530 (G6R31_01530) pilB 293411..296074 (+) 2664 WP_017870162.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  G6R31_RS01535 (G6R31_01535) pilT 296113..297375 (+) 1263 WP_114671147.1 type IV pilus twitching motility protein PilT Machinery gene
  G6R31_RS01540 (G6R31_01540) - 297487..297999 (-) 513 WP_017870164.1 hypothetical protein -
  G6R31_RS01545 (G6R31_01545) - 298256..300898 (+) 2643 WP_017870165.1 S8 family serine peptidase -
  G6R31_RS01550 (G6R31_01550) - 301072..301737 (-) 666 WP_017870167.1 ankyrin repeat domain-containing protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 44963.31 Da        Isoelectric Point: 6.7738

>NTDB_id=426261 G6R31_RS01535 WP_114671147.1 296113..297375(+) (pilT) [Deinococcus wulumuqiensis R12]
MTMTSGIDITDILRVAADQGASDVILAAGLPPQFKLSGTYDSQGFEALSPTDTRKLMYSMMNERQQRTFEEKRELDFSFA
LGDKARFRVNTFMQRGNVGGVMRLIPTTVRTIAEMGLPQTVIDIASAPRGLVLVTGPTGSGKSTTLASMIDHINTTKKLH
ILTIEDPIEFMHPNKASIINQREVGADTLSFDDALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAP
ESIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRADGQGRVLAYEILLANPAVRALIREGKTFQITSVMQTGAREGM
VTMDAYLAGLFRKHLITYDKGLERAIDPKEFARLANDPSAGLGGAAMTPAAPPVPAYSSAASSGAGRGGDFGRGAAAPAA
PATPASGRNDPGRPGGFGRR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=426261 G6R31_RS01535 WP_114671147.1 296113..297375(+) (pilT) [Deinococcus wulumuqiensis R12]
ATGACCATGACTTCCGGTATCGACATCACCGACATCCTGCGCGTCGCCGCCGACCAGGGCGCGTCCGACGTCATCCTCGC
CGCCGGACTGCCCCCGCAGTTCAAGCTCTCGGGCACCTACGACTCGCAGGGCTTCGAAGCCCTGTCGCCCACCGACACCC
GCAAGCTGATGTACTCGATGATGAACGAGCGCCAGCAGCGCACCTTCGAGGAAAAGCGCGAACTCGACTTTTCCTTCGCG
CTGGGCGACAAGGCCCGTTTCCGCGTGAACACCTTCATGCAGCGCGGCAATGTGGGCGGCGTCATGCGACTGATTCCCAC
CACCGTCCGCACCATCGCCGAGATGGGGCTGCCGCAGACCGTCATCGACATCGCGAGCGCCCCGCGTGGGCTGGTGCTGG
TGACGGGGCCGACGGGGTCGGGCAAATCGACCACCCTGGCGTCGATGATCGACCACATCAACACCACCAAGAAGCTGCAC
ATCCTGACCATCGAAGACCCCATCGAGTTCATGCACCCCAACAAGGCCAGCATCATCAACCAGCGCGAGGTCGGGGCCGA
CACCCTGAGTTTCGACGACGCGCTGCGGGCCGCGCTGCGGCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGTGACT
ACGAAACCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTCGTGATGGGCACGCTGCACACCAACTCGGCGCCC
GAATCCATCGACCGTATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAACAGATTCGCGTGCAGCTCGCCAACAACCTCGT
CGCGGTGATGACCCAGCAGCTTCTGCCCCGCGCCGACGGTCAGGGCCGCGTGCTGGCCTACGAGATTCTGCTGGCGAACC
CGGCAGTCCGCGCCCTGATTCGTGAGGGCAAGACCTTCCAGATCACCAGCGTGATGCAGACCGGCGCCCGCGAGGGCATG
GTCACCATGGACGCCTACCTCGCCGGACTGTTCCGCAAGCACCTGATCACCTACGACAAGGGCCTGGAACGTGCCATCGA
CCCCAAGGAATTCGCCCGCCTCGCCAACGACCCCTCGGCGGGTCTGGGGGGCGCGGCCATGACGCCCGCCGCGCCGCCGG
TTCCGGCCTACAGCAGCGCGGCCAGCAGCGGTGCCGGGCGCGGCGGCGACTTCGGACGGGGTGCGGCGGCCCCGGCAGCT
CCGGCCACGCCCGCGTCGGGTCGCAACGACCCCGGACGCCCTGGGGGGTTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

94.019

99.524

0.936
  pilT Pseudomonas stutzeri DSM 10701

52.754

82.143

0.433
  pilT Acinetobacter baylyi ADP1

52.174

82.143

0.429
  pilT Pseudomonas aeruginosa PAK

52.174

82.143

0.429
  pilT Neisseria meningitidis 8013

52.478

81.667

0.429
  pilT Acinetobacter nosocomialis M2

51.884

82.143

0.426
  pilT Acinetobacter baumannii strain A118

51.884

82.143

0.426
  pilT Acinetobacter baumannii D1279779

51.884

82.143

0.426
  pilT Neisseria gonorrhoeae MS11

52.212

80.714

0.421
  pilT Legionella pneumophila strain Lp02

48.986

82.143

0.402
  pilT Legionella pneumophila strain ERS1305867

48.986

82.143

0.402
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.201

75.714

0.395
  pilT Vibrio cholerae strain A1552

52.201

75.714

0.395